Digoxigenin
Based on 2 publication(s) in Google Scholar
Digoxigenin (DIG) is a steroid. DIG is used for situ hybridization as a labeling molecule probe due to long shelf life and fast detection and high sensitivity of DIG-labeled riboprobes.
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- Pureza: 99.60%
- No. CAS: 1672-46-4
- Fòrmula: C23H34O5
- Peso molecular:390.51
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Almacenamiento:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Digoxigenin
More
Actividad biológica
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HT-29 | IC50 |
3.6 μM
Compound: 5
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Cytotoxicity against human HT-29 cells after 72 hrs by CellTiter 96 Aqueous One Solution reagent based assay
Cytotoxicity against human HT-29 cells after 72 hrs by CellTiter 96 Aqueous One Solution reagent based assay
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[PMID: 32096998] |
| MCF7 | IC50 |
507 nM
Compound: 4
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Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay
Cytotoxicity against human MCF7 cells after 48 hrs by SRB assay
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[PMID: 16309315] |
| MDA-MB-231 | IC50 |
3.2 μM
Compound: 5
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Cytotoxicity against human MDA-MB-231 cells after 72 hrs by CellTiter 96 Aqueous One Solution reagent based assay
Cytotoxicity against human MDA-MB-231 cells after 72 hrs by CellTiter 96 Aqueous One Solution reagent based assay
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[PMID: 32096998] |
| MDA-MB-435 | IC50 |
0.9 μM
Compound: 5
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Cytotoxicity against human MDA-MB-435 cells after 72 hrs by CellTiter 96 Aqueous One Solution reagent based assay
Cytotoxicity against human MDA-MB-435 cells after 72 hrs by CellTiter 96 Aqueous One Solution reagent based assay
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[PMID: 32096998] |
| MDCK | IC50 |
0.49 μM
Compound: Digoxigenin
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TP_TRANSPORTER: inhibition of Digoxin uptake in OATP4C1-expressing MDCK cells
TP_TRANSPORTER: inhibition of Digoxin uptake in OATP4C1-expressing MDCK cells
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[PMID: 14993604] |
| OVCAR-3 | IC50 |
2.4 μM
Compound: 5
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Cytotoxicity against human OVCAR3 cells after 72 hrs by CellTiter 96 Aqueous One Solution reagent based assay
Cytotoxicity against human OVCAR3 cells after 72 hrs by CellTiter 96 Aqueous One Solution reagent based assay
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[PMID: 32096998] |
| TK-10 | IC50 |
317.5 nM
Compound: 4
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Cytotoxicity against human TK10 cells after 48 hrs by SRB assay
Cytotoxicity against human TK10 cells after 48 hrs by SRB assay
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[PMID: 16309315] |
| UACC-62 | IC50 |
416.1 nM
Compound: 4
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Cytotoxicity against human UACC62 cells after 48 hrs by SRB assay
Cytotoxicity against human UACC62 cells after 48 hrs by SRB assay
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[PMID: 16309315] |
Digoxigenin is a potent activator of RORγ-dependent transcription at 1 μM with an EC50 value of 0.358 μM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HepG2 (human hepatocellular carcinoma) cell line
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Concentration:0-10 μM
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Incubation Time:24 h
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Result:Identified the optimal concentration for Digoxigenin: 1 μM.
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Cell Line:RORγ-HepG2 cells
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Concentration:0-2 μM
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Incubation Time:24 h
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Result:Increased the luciferase activity with concentration-dependent in cells.
Chemical Information
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No. CAS 1672-46-4
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Appearance Solid
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Peso molecular 390.51
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Fòrmula C23H34O5
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Color White to off-white
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SMILES
O=C1OCC([C@H]2CC[C@]3(O)[C@]4([H])CC[C@]5([H])C[C@@H](O)CC[C@]5(C)[C@@]4([H])C[C@@H](O)[C@]23C)=C1
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Synonyms
Lanadigenin
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Structure Classification
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (2)
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Journal Impact Factor
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Most Recent
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Antiviral Res
Repurposing screen using a robust human rhinovirus infectious clone identifies pyrvinium pamoate with antiviral activity. [Abstract]2026 Jun:250:106417. PMID: 42025967 -
J Pharm Biomed Anal
Integrated pharmacokinetics and multidimensional drug-drug interactions assessment of bufadienolides based on UPLC-QqQ-MS/MS. [Abstract]2025 Dec 15:266:117118. PMID: 40848656
Solvente y solubilidad
DMSO : 100 mg/mL (256.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureza y Documentación
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Ficha de datos (278 KB)
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SDS (420 KB)
- English - EN (420 KB)
- Français - FR (420 KB)
- Deutsch - DE (420 KB)
- Norwegian - NO (420 KB)
- Español - ES (420 KB)
- Swedish - SV (420 KB)
- Italian - IT (420 KB)
- Korean - KR (420 KB)
- Portuguese - PT (420 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Luo, Yuhao, et al. Long noncoding RNA (lncRNA) EIF3J-DT induces chemoresistance of gastric cancer via autophagy activation. Autophagy vol. 17,12 (2021): 4083-4101. [Content Brief]
[2]. Karaś, Kaja, et al. The cardenolides strophanthidin, digoxigenin and dihydroouabain act as activators of the human RORγ/RORγT receptors. Toxicology letters vol. 295 (2018): 314-324. [Content Brief]
[3]. Barratt, Kristen S, et al. Production of Digoxigenin-Labeled Riboprobes for In Situ Hybridization Experiments. Current protocols in mouse biology vol. 10,2 (2020): e74. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.5608 mL | 12.8038 mL | 25.6075 mL | 64.0188 mL |
| 5 mM | 0.5122 mL | 2.5608 mL | 5.1215 mL | 12.8038 mL | |
| 10 mM | 0.2561 mL | 1.2804 mL | 2.5608 mL | 6.4019 mL | |
| 15 mM | 0.1707 mL | 0.8536 mL | 1.7072 mL | 4.2679 mL | |
| 20 mM | 0.1280 mL | 0.6402 mL | 1.2804 mL | 3.2009 mL | |
| 25 mM | 0.1024 mL | 0.5122 mL | 1.0243 mL | 2.5608 mL | |
| 30 mM | 0.0854 mL | 0.4268 mL | 0.8536 mL | 2.1340 mL | |
| 40 mM | 0.0640 mL | 0.3201 mL | 0.6402 mL | 1.6005 mL | |
| 50 mM | 0.0512 mL | 0.2561 mL | 0.5122 mL | 1.2804 mL | |
| 60 mM | 0.0427 mL | 0.2134 mL | 0.4268 mL | 1.0670 mL | |
| 80 mM | 0.0320 mL | 0.1600 mL | 0.3201 mL | 0.8002 mL | |
| 100 mM | 0.0256 mL | 0.1280 mL | 0.2561 mL | 0.6402 mL |
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