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Dihydroethidium

(Synonyms: Hydroethidine; PD-MY 003)
188 Cited Publications
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Based on 188 publication(s) in Google Scholar

Dihydroethidium, also known as DHE, is a peroxide indicator. Dihydroethidium penetrates cell membranes to form a fluorescent protein complex with blue fluoresces. After entering the cells, Dihydroethidium is mainly localized in the cell membrane, cytoplasm and nucleus, and the staining effect is the strongest in the nucleus. Dihydroethidium produces inherent blue fluorescence with a maximum excitation wavelength of 370 nm and a maximum emission wavelength of 420 nm; after dehydrogenation, Dihydroethidium combines with RNA or DNA to produce red fluorescence with a maximum excitation wavelength of 300 nm and a maximum emission wavelength of 610 nm. 535 nm can also be used as the excitation wavelength for actual observation.

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  • Pureza: 98.83%
  • No. CAS: 104821-25-2
  • Fòrmula: C21H21N3
  • Peso molecular:315.42
  • Almacenamiento:

    4°C, protect from light

    * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

  • Dihydroethidium Chemical Structure
    Dihydroethidium Chemical Structure
  • Experimental Validation Results for Dihydroethidium
  • Experimental Validation Results for Dihydroethidium
  • Experimental Validation Results for Dihydroethidium
  • Experimental Validation Results for Dihydroethidium
  • Experimental Validation Results for Dihydroethidium
  • Experimental Validation Results for Dihydroethidium
  • Experimental Validation Results for Dihydroethidium
  • Experimental Validation Results for Dihydroethidium
No. CAS: 104821-25-2
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  • Dihydroethidium Chemical Structure
    Dihydroethidium Chemical Structure
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    Dihydroethidium purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2023 May 1:200:73-86. [Abstract]

    Customer Validation
     The Dihydroethidium (DHE; 10 μM; 1 h), Dihydrorhodamine 123 (DHR 123, 5 μM; 1 h), FerroOrange and C11 BODIPY probes detect O2• − (red fluorescence),OONO − (green fluorescence), Fe 2+ (orange fluorescence), and lipid peroxides (red fluorescence in the unoxidized state and green fluorescence in the oxidized state), respectively, In normal lung tissue from 10 smoking patients and 10 non-smoking patients.

  • 1/16

    Dihydroethidium purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2023 May 1:200:73-86. [Abstract]

    Customer Validation
     The Dihydroethidium (DHE; 5-20 μM; 1 h）, Dihydrorhodamine 123 (DHR 123, 5 μM, 15-60 min) probes were used to detect ROS intensities in CS-induced bronchial epithelial ferroptosis. Control, and 0.5%,1%, and 2% CSE were used to stimulate BEAS-2B cells for 48 h.

  • 1/16

    Dihydroethidium purchased from MedChemExpress. Usage Cited in: Antioxidants (Basel). 2022 Aug 19;11(8):1608. [Abstract]

    Customer Validation
     Superoxide anion production was measured by using the fluorescent dye Dihydroethidium (DHE). The DHE fluorescence assay detected nuclear O2●− through a chemical reaction between superoxide and DHE, whereby the latter was oxidized to ethidium.

  • 1/16

    Dihydroethidium purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2022 Aug 4;2022:6160701. [Abstract]

    Customer Validation
     Brain sections are incubated in 10 μM Dihydroethidium (DHE) solution for 1 h in dark at 37 °C.

  • 1/16

    Dihydroethidium purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2021 Jun 20:2021:5546711. [Abstract]

    Customer Validation
     HUVECs are washed with M199 and stained with Dihydroethidium (DHE, 10 μM) at 37°C with 5% CO2 for 30 min. After washing 3 times with M199, ethidium fluorescence is observed and photographed under an inversion fluorescence microscope.

  • 1/16

    Dihydroethidium purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2021 Aug 31;2021:5608133. [Abstract]

    Customer Validation
     Nucleus pulposus (NP) slices are incubated with a culture medium containing 10 μM Dihydroethidium and 1 μg/mL calcein AM for one hour at 37°C. After washing with PBS, the slices are immediately viewed using an inverted confocal laser scanning microscope.

  • 1/16

    Dihydroethidium purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2020 Apr 20;11(4):256. [Abstract]

    Customer Validation
     The levels of ROS production in the liver tissues are determined by Dihydroethidium (DHE) staining as previously described. In brief, frozen sections of liver tissues in each group (5-μm-thick) are prepared and incubated with DHE (7.5 mM) for 30 min in the dark at 37 °C.

  • 1/16

    Dihydroethidium purchased from MedChemExpress. Usage Cited in: Antioxidants (Basel). 2020 Sep 18;9(9):885. [Abstract]

    Customer Validation
     Paraffin sections (5 μm) were used to measure retinal and choroidal ROS production using a fluorescent dye with Dihydroethidium (DHE) for 20 min at 37 °C.

  • Dihydroethidium