SANT-1
Based on 4 publication(s) in Google Scholar
SANT-1, a potent Smo antagonist, inhibits Hedgehog signaling. SANT-1 shows IC50s of 20 nM and 30 nM in Shh-LIGHT2 and SmoA1-LIGHT2 assay, respectively.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.52%
- No. CAS: 304909-07-7
- Fòrmula: C23H27N5
- Peso molecular:373.49
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) SANT-1
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Customer Validation & Images
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IHC
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IHC
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IHC
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RT-PCR
Actividad biológica
IC50: 20 nM (Shh signaling), 30 nM (SmoA1)[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| C3H 10T1/2 | IC50 |
20 nM
Compound: 50, SANT-1
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Inhibition of SHH-mediated mouse C3H10T1/2 cells differentiation into osteoblasts by alkaline phosphatase assay
Inhibition of SHH-mediated mouse C3H10T1/2 cells differentiation into osteoblasts by alkaline phosphatase assay
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[PMID: 19309080] |
| HEK293 | IC50 |
25 nM
Compound: 4, SANT-1
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Inhibition of Smoothened transfected in HEK293 cells by binding assay
Inhibition of Smoothened transfected in HEK293 cells by binding assay
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[PMID: 20620058] |
| HEK293 | IC50 |
4.74 nM
Compound: 4, SANT-1
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Inhibition of Smoothened transfected in HEK293 cells by transient transfection cell-based assay
Inhibition of Smoothened transfected in HEK293 cells by transient transfection cell-based assay
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[PMID: 20620058] |
SANT-1 is a potent Smo antagonist, binds directly to Smo, and inhibits Hedgehog signaling, with IC50s of 20 nM and 30 nM in Shh-LIGHT2 and SmoA1-LIGHT2 cells, respectively[1].
SANT-1 (1 μM, for 24 or 48 hours) decreases the proliferation of medulloblastoma cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Medulloblastoma cells
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Concentration:1 μM
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Incubation Time:24 hours or 48 hours
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Result:Inhibited cellular proliferation in primary tumorspheres.
Chemical Information
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No. CAS 304909-07-7
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Appearance Solid
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Peso molecular 373.49
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Fòrmula C23H27N5
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Color White to off-white
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SMILES
CC1=C(/C=N/N2CCN(CC3=CC=CC=C3)CC2)C(C)=NN1C4=CC=CC=C4
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (4)
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Journal Impact Factor
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Most Recent
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Front Cell Dev Biol
miR-212/132-Enriched Extracellular Vesicles Promote Differentiation of Induced Pluripotent Stem Cells Into Pancreatic Beta Cells. [Abstract]2021 May 13:9:673231. PMID: 34055806 -
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Biomed Pharmacother
Interference of sympathetic overactivation restores limbal stem/progenitor cells function and accelerates corneal epithelial wound healing in diabetic mice. [Abstract]2023 May:161:114523. PMID: 36931034
SANT-1 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 May:161:114523. [Abstract]
The promotion effect of ICI on diabetic epithelial regeneration was large partially reversed by Sant-1 (250 nM).
SANT-1 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 May:161:114523. [Abstract]
Ki67 staining showed that ICI promoted proliferation in LSPCs were totally reversed by Sant-1 (250 nM).
SANT-1 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 May:161:114523. [Abstract]
The elevation effect of ICI on ΔNp63, Krt15 and Gpha2 expression in diabetic wounded corneal limbus were reversed by Sant-1 (250 nM).
SANT-1 purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2023 May:161:114523. [Abstract]
The elevation effect of ICI on ΔNp63, Krt15 and Gpha2 expression in diabetic wounded corneal limbus were reversed by Sant-1 (250 nM).
Solvente y solubilidad
DMSO : 25 mg/mL (66.94 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.69 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.57 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (271 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6774 mL | 13.3872 mL | 26.7745 mL | 66.9362 mL |
| 5 mM | 0.5355 mL | 2.6774 mL | 5.3549 mL | 13.3872 mL | |
| 10 mM | 0.2677 mL | 1.3387 mL | 2.6774 mL | 6.6936 mL | |
| 15 mM | 0.1785 mL | 0.8925 mL | 1.7850 mL | 4.4624 mL | |
| 20 mM | 0.1339 mL | 0.6694 mL | 1.3387 mL | 3.3468 mL | |
| 25 mM | 0.1071 mL | 0.5355 mL | 1.0710 mL | 2.6774 mL | |
| 30 mM | 0.0892 mL | 0.4462 mL | 0.8925 mL | 2.2312 mL | |
| 40 mM | 0.0669 mL | 0.3347 mL | 0.6694 mL | 1.6734 mL | |
| 50 mM | 0.0535 mL | 0.2677 mL | 0.5355 mL | 1.3387 mL | |
| 60 mM | 0.0446 mL | 0.2231 mL | 0.4462 mL | 1.1156 mL |
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