Actagardin
Actagardin is a tetracyclic lantibiotic produced by several species of Actinoplanes. It is composed of macrocyclic rings formed by thioether bridges. Actagardin preferably targets Gram-positive bacteria, inhibiting the synthesis of peptidoglycan.
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- No. CAS: 59165-34-3
- Fòrmula: C81H124N20O24S4
- Peso molecular:1890.23
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
Descripciòn
Chemical Information
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No. CAS 59165-34-3
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Peso molecular 1890.23
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Fòrmula C81H124N20O24S4
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Synonyms
Antibiotic A 3802-IV-3; Gardimycin
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Sequence
{Cys}{Ser}{Gly}{Trp}{Val}{Cys}{Cys}{Leu}{Cys}{Ile}{Cys}{Gly}{Cys}{Val}{Ile}{Cys}{Ala}{Cys} (Carba sulfide bridge:Cys1-Cys6,Cys7-Cys12,Cys9-Cys17,Cys14-Cys19)
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Sequence Shortening
CSGWVCCLCICGCVICAC (Carba sulfide bridge:Cys1-Cys6,Cys7-Cys12,Cys9-Cys17,Cys14-Cys19)
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocolo
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Gram Staining of Tissue Sections
Gram staining of tissue sections is a histochemical technique used to differentiate Gram-positive and Gram-negative bacteria within histological specimens based on differences in bacterial cell wall structure and dye retention, adapted from classical bacteriological Gram staining into tissue-compatible “histological Gram stain” variants. In tissue applications, modifications of the Brown-Hopps and Brown-Brenn methods are commonly used to improve differentiation of microorganisms embedded within host connective tissue and to reduce overstaining or loss of Gram-negative signal, which are known limitations of earlier approaches. The principle relies on crystal violet-iodine complex retention in Gram-positive organisms and subsequent decolorization and counterstaining steps that allow contrast visualization of Gram-negative organisms against tissue background.
Pureza y Documentación
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)