Boscalid
Based on 1 publication(s) in Google Scholar
Boscalid is a succinate dehydrogenase (SDHI) inhibitor with antifungal activity. Boscalid binds to the ubiquinone-binding site of fungal mitochondrial complex II, blocks ATP production and aerobic respiration, exhibits good control efficacy against a variety of plant fungal diseases including gray mold, sclerotinia rot and powdery mildew, and is widely used for disease control in agriculture. Boscalid induces apoptosis, altered lipid metabolism, mitochondrial dysfunction, respiratory impairment, oxidative stress, ROS accumulation and neurodevelopmental disorders in zebrafish. Boscalid reduces foraging ability, shortens median death time and causes chronic toxicity in exposed honeybees. Boscalid also possesses genotoxicity, cytotoxicity, elevated mitochondrial superoxide levels and early-stage apoptosis.
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- Pureza: 99.80%
- No. CAS: 188425-85-6
- Fòrmula: C18H12Cl2N2O
- Peso molecular:343.21
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Boscalid
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Actividad biológica
succinate dehydrogenase (SDH)[1]
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Tg(elavl3: EGFP) transgenic line, AB strain[2]
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Dosage:5 mg/L; 15 mg/L; 25 mg/L
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Administration:waterborne; 6 to 48 hpf (embryos); 24 h (6 dpf larvae)
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Result:Observed obscure brain regional division, blurred ventricles, malformed eyes, and decreased spontaneous tail coiling frequency at 24 hpf in embryos. Detected enlarged cerebral ventricles, reduced pigmentation, decreased neonatal neurons, defective development around ventricles, increased brain intercellular space, and concentrated nuclei at 48 hpf in embryos. Measured increased Na⁺K⁺-ATPase, Ca²⁺Mg²⁺-ATPase, and total ATPase activities; increased MDA content; accumulated ROS in head and heart; increased CAT activity; decreased SOD activity; decreased tyrosinase activity in embryos. Recorded decreased expression of tfap2a, tfap2e, sox10, gfap, gap43; increased then decreased expression of wnt1, mitfa, pax3a; increased expression of sliva, oca2, nestin, neurogenin1 in embryos. Found increased central area motion proportion, increased angular velocity and sinuosity; decreased total distance, average speed, movement duration, mobile/high mobile frequency and duration in larvae.
Chemical Information
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No. CAS 188425-85-6
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Appearance Solid
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Peso molecular 343.21
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Fòrmula C18H12Cl2N2O
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Color White to off-white
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SMILES
ClC1=CC=C(C=C1)C2=CC=CC=C2NC(C3=C(N=CC=C3)Cl)=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
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J Agric Food Chem
Transcription Repressor SsGATA2 Regulates Broad-Spectrum Resistance to Fungicides and Pathogenicity in Sclerotinia sclerotiorum. [Abstract]2025 Jul 9;73(27):16787-16803. PMID: 40558024
Solvente y solubilidad
DMSO : 100 mg/mL (291.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.28 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (280 KB)
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SDS (458 KB)
- English - EN (458 KB)
- Français - FR (458 KB)
- Deutsch - DE (458 KB)
- Norwegian - NO (458 KB)
- Español - ES (458 KB)
- Swedish - SV (458 KB)
- Italian - IT (458 KB)
- Korean - KR (458 KB)
- Portuguese - PT (458 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Moschopoulou G, et al. Development of a Portable Cell-Based Biosensor for the Ultra-Rapid Screening for Boscalid Residues in Lettuce. Biosensors (Basel). 2024;14(6):311. Published 2024 Jun 18. [Content Brief]
[2]. Wang H, et al. Characterization of boscalid-induced oxidative stress and neurodevelopmental toxicity in zebrafish embryos. Chemosphere. 2020;238:124753. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9137 mL | 14.5683 mL | 29.1367 mL | 72.8417 mL |
| 5 mM | 0.5827 mL | 2.9137 mL | 5.8273 mL | 14.5683 mL | |
| 10 mM | 0.2914 mL | 1.4568 mL | 2.9137 mL | 7.2842 mL | |
| 15 mM | 0.1942 mL | 0.9712 mL | 1.9424 mL | 4.8561 mL | |
| 20 mM | 0.1457 mL | 0.7284 mL | 1.4568 mL | 3.6421 mL | |
| 25 mM | 0.1165 mL | 0.5827 mL | 1.1655 mL | 2.9137 mL | |
| 30 mM | 0.0971 mL | 0.4856 mL | 0.9712 mL | 2.4281 mL | |
| 40 mM | 0.0728 mL | 0.3642 mL | 0.7284 mL | 1.8210 mL | |
| 50 mM | 0.0583 mL | 0.2914 mL | 0.5827 mL | 1.4568 mL | |
| 60 mM | 0.0486 mL | 0.2428 mL | 0.4856 mL | 1.2140 mL | |
| 80 mM | 0.0364 mL | 0.1821 mL | 0.3642 mL | 0.9105 mL | |
| 100 mM | 0.0291 mL | 0.1457 mL | 0.2914 mL | 0.7284 mL |
Boscalid Related Classifications
HY-119976 Related Classifications
Powered by Bioz
- Boscalid
- 188425-85-6
- Environmental Pollutants
- Mitochondrial Metabolism
- Fungal
- Apoptosis
- Succinate Dehydrogenase
- Alternaria alternata
- HepG2 liver cells
- Sclerotinia sclerotiorum
- Caco-2/HT29-MTX/Raji B cells
- Chlorella vulgaris
- honeybees
- fungal mitochondrial complex II
- succinate dehydrogenase inhibitor
- zebrafish
- Blumeriella jaapii
- Inhibitor
- inhibitor
- inhibit