DASPEI

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Preparation of DASPEI working solution
1.1 Preparation of the stock solution
Dissolve 1 mg of DASPEI in 0.2016 mL of DMSO to obtain 10 mM of DASPEI
Note: It is recommended to store the stock solution at -20°C -80°C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of DASPEI working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 0.5-1 mM of DASPEI working solution.
Note: Please adjust the concentration of DASPEI working solution according to the actual situation.
2. Cell staining
2.1 For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.3 Add 1 mL of DASPEI working solution, and then incubate at room temperature for 10-30 minutes.
2.4 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.5 Wash twice with PBS, 5 minutes each time.
2.6 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.