HOTPy
HOTPy is a mitochondria-targeted fluorescent viscosity probe with aggregation-induced emission properties, which responds to microenvironmental viscosity. HOTPy can recognize the abnormal increase in mitochondrial viscosity and capture dynamic viscosity changes in acute alcoholic liver injury models at both cellular and in vivo levels. The maximum absorption peak of HOTPy in PBS solution is at 379 nm; its maximum emission peak reaches 611 nm in a high-viscosity glycerol system. HOTPy can be used for the research of acute alcoholic liver injury.
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- No. CAS: 2763752-95-8
- Fòrmula: C32H26INO
- Peso molecular:567.46
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
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Chemical Information
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Actividad biológica
Descripciòn
In Vitro
1. Stock Solution Preparation
1.2 Recommended concentration: 40 μM.
2. Working Solution Preparation
2.1 Diluent: Phosphate-buffered saline (PBS, 10 mM, pH 7.4).
2.2 Working concentration: 10 μM.
2.3 Notes: Adjust the working solution concentration as needed; prepare and use immediately.
3. Staining Procedure
3.1 Sample Type Description
3.1.1 Adherent cells[1]: Human liver cells (LO2 cells); trypsin digestion is not required before staining.
3.2 Incubation Conditions
3.2.1 Adherent cells: Incubate with 10 μM HOTPy at 37°C for 30 min.
3.3 Washing Steps
3.3.1 Adherent cells: After incubation, wash 3 times with 1 mL PBS at room temperature.
4. Control Setup
4.1 Cell Imaging Controls:
4.1.1 Negative control: LO2 cells without HOTPy incubation.
4.1.2 Positive control: LO2 cells pretreated with Monensin (HY-N4302) (10 μM), Nystatin (HY-17409) (10 μM) or ethanol (0.8 M) for 60 min before HOTPy incubation.
5. Detection and Analysis
5.1 Instrument type: Confocal laser scanning microscope.
5.2 Excitation/Emission Wavelengths
5.2.1 Cell imaging: Ex = 430 nm, Em = 600-700 nm (corresponding to the red channel).
5.3 Result Analysis
5.3.1 Fluorescence intensity change: An increase in red fluorescence intensity indicates elevated mitochondrial viscosity in cells or liver tissues; a decrease in fluorescence intensity indicates reduced viscosity.
5.3.2 Fluorescence localization: Red fluorescence shows a punctate distribution in mitochondria of LO2 cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
1. Stock Solution Preparation
1.2 Recommended concentration: 40 μM.
2. Working Solution Preparation
2.1 Diluent: Phosphate-buffered saline (PBS, 10 mM, pH 7.4).
2.2 Working concentration: 10 μM.
2.3 Notes: Adjust the working solution concentration as needed; prepare and use immediately.
3. Staining Procedure
3.1 Sample Type Description
3.1.1 In vivo animal samples[1]: Male BALB/c mice.
3.2 Incubation Conditions
3.2.1 In vivo animal samples: Acute alcoholic liver injury mouse model, injected with 10 μM HOTPy (0.2 mL) via tail vein; image after 30 min.
4. Control Setup
4.1 In vivo imaging controls:
4.1.1 Normal control mice: Untreated mice.
4.1.2 Model control mice: Acute alcoholic liver injury mice without intervention.
4.1.3 Vehicle control mice: Acute alcoholic liver injury mice treated with PBS instead of the intervention drug.
5. Detection and Analysis
5.1 Instrument types: In vivo imaging system; fluorescence imager.
5.2 Result Analysis
5.2.1 Changes in fluorescence intensity: Increased red fluorescence intensity indicates elevated mitochondrial viscosity in cells or liver tissues; decreased fluorescence intensity indicates reduced viscosity.
5.2.2 Fluorescence localization: In vivo fluorescence is mainly localized in the liver of mice.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 2763752-95-8
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Peso molecular 567.46
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Fòrmula C32H26INO
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SMILES
C[N+]1=CC=C(C=C1)C2=CC=C(C=C2)/C(C3=CC=CC=C3)=C(C4=CC=C(C=C4)O)\C5=CC=CC=C5.[I-]
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
HOTPy Related Classifications
HY-D3121 Related Classifications
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