LR004
LR004 is an EGFR monoclonal antibody, with a Kd of 2.80×10-9 M against human EGFR. LR004 shows extremely weak inhibitory effect on the viability of EGFR-positive tumor cells in vitro, but inhibits the growth of EGFR-positive tumor xenografts as a single agent. LR004 is applicable to research related to advanced colorectal cancer, solid tumors, esophageal squamous cell carcinoma, epidermoid carcinoma, colon cancer and breast cancer.
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
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Chemical Information
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Actividad biológica
Human
LR004 (5.0 μg/mL) binds recombinant human EGFR antigen with moderate affinity, with a Kd of 2.80×10-9 M[1].
LR004 (0.023-50 μg/mL; 24 h initial incubation, followed by 96 h incubation with serum-containing medium) inhibits the proliferation of MDA-MB-468 human breast cancer cells with an EC50 of 2.278 μg/mL[1].
LR004 (0.001-1 μg·mL-1; 48 h) has weak in vitro cytotoxicity against human EGFR-positive tumor cells (KYSE520, KYSE150, A431, HCC827, NCI-H1975, A549, MDA-MB-468, MCF-7, AsPC-1) and EGFR-negative Karpas 299 cells, with IC50 values >6 nM or >60 nM, respectively[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MDA-MB-468 human breast cancer cells
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Concentration:0.023-50 μg/mL
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Incubation Time:24 h (initial incubation); 96 h (incubation with serum-containing medium)
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Result:Inhibited MDA-MB-468 cell proliferation with an EC50 of 2.278 μg/mL.
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Cell Line:KYSE520, KYSE150, A431, HCC827, NCI-H1975, A549, MDA-MB-468, MCF-7, AsPC-1, Karpas 299
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Concentration:0.001-1 μg·mL-1
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Incubation Time:48 h
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Result:Had a weak inhibitory effect on cell viability, with IC50 values >6 nM for KYSE520, KYSE150, A431, HCC827, NCI-H1975, A549, MDA-MB-468, MCF-7, and AsPC-1 cells, and IC50 >60 nM for Karpas 299 cells.
LR004 (15 mg/kg; i.v.; every 4 days; 6 total doses) exerts limited tumor growth suppression in A431 epidermoid carcinoma xenografts[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c nude (female, 6-8 weeks old, 18-20 g, KYSE520 tumor cell xenograft)[2]
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Dosage:15 mg/kg
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Administration:i.v.; every 4 days; 4 total doses
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Result:Achieved an average tumor volume of 1791 mm3 on day 44.
Induced a 13.8% tumor inhibition rate.
Showed no tumor regression.
Caused no body weight loss or mortality.
Detected no histopathological abnormalities in major organs.
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Animal Model:BALB/c nude (female, 6-8 weeks old, 18-20 g, A431 tumor cell xenograft)[2]
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Dosage:15 mg/kg
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Administration:i.v.; every 4 days; 6 total doses
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Result:Achieved an average tumor volume of 399 mm3 on day 60.
Exhibited limited therapeutic activity compared to LR004-VC-MMAE groups.
Detected no histopathological abnormalities in major organs.
P00533-1
EGFR
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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Product Image
ELISA, FACS, Functional assay
Chemical Information
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SMILES
[LR004]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
[1]. Zheng Y, et al. Preclinical Pharmacokinetics and Biodistribution of LR004, a Novel Antiepidermal Growth Factor Receptor Monoclonal Antibody. Molecules. 2024;29(2):545. Published 2024 Jan 22. [Content Brief]
[2]. Hu XY, et al. An EGFR-targeting antibody-drug conjugate LR004-VC-MMAE: potential in esophageal squamous cell carcinoma and other malignancies. Mol Oncol. 2019;13(2):246-263. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
LR004 Related Classifications
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