Physalin F
Based on 1 Customer Validation
Physalin F is a secosteroid with potent anti-inflammatory and immunomodulatory activities. Physalin F induces apoptosis of PBMC, decreasing the spontaneous proliferation and cytokine production caused by Human T-lymphotropic virus type 1 (HTLV-1) infection.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.98%
- No. CAS: 57423-71-9
- Fòrmula: C28H30O10
- Peso molecular:526.53
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Almacenamiento:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Apoptosis Compound Library
HY-L003
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Actividad biológica
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
>50 μM
Compound: 22
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Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
Antiproliferative activity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
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[PMID: 35608269] |
| HepG2 | IC50 |
>50 μM
Compound: 22
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Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
Antiproliferative activity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
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[PMID: 35608269] |
| HT-1080 | IC50 |
2.41 μM
Compound: Physalin F
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Antiproliferative activity against human HT-1080 cells assessed as reduction in cell proliferation measured after 48 hrs by CCK8 assay
Antiproliferative activity against human HT-1080 cells assessed as reduction in cell proliferation measured after 48 hrs by CCK8 assay
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[PMID: 33735687] |
| L02 | IC50 |
>50 μM
Compound: 22
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Cytotoxicity against human L02 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
Cytotoxicity against human L02 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
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[PMID: 35608269] |
| MCF7 | IC50 |
>50 μM
Compound: 22
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Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
Antiproliferative activity against human MCF7 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
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[PMID: 35608269] |
| MCF7 | IC50 |
0.6 μM
Compound: 8
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Cytotoxicity against human MCF7 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay
Cytotoxicity against human MCF7 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay
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[PMID: 33586438] |
| NCI-H460 | IC50 |
1 μM
Compound: 8
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Cytotoxicity against human NCI-H460 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay
Cytotoxicity against human NCI-H460 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay
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[PMID: 33586438] |
| PC-3 | IC50 |
1 μM
Compound: 8
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Cytotoxicity against human PC-3 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay
Cytotoxicity against human PC-3 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay
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[PMID: 33586438] |
| RAW264.7 | IC50 |
>50 μM
Compound: 22
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Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
Cytotoxicity against mouse RAW264.7 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
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[PMID: 35608269] |
| RAW264.7 | IC50 |
7.9 μM
Compound: 22
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Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-stimulated NO production pretreated with LPS followed by compund addition by Griess reagent based microplate reader assay
Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-stimulated NO production pretreated with LPS followed by compund addition by Griess reagent based microplate reader assay
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[PMID: 35608269] |
| SF-268 | IC50 |
0.5 μM
Compound: 8
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Cytotoxicity against human SF-268 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay
Cytotoxicity against human SF-268 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay
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[PMID: 33586438] |
| SGC-7901 | IC50 |
>50 μM
Compound: 22
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Antiproliferative activity against human SGC-7901 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
Antiproliferative activity against human SGC-7901 cells assessed as reduction in cell viability incubated for 48 hrs by CCK8 assay
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[PMID: 35608269] |
| WI-38 | IC50 |
3.5 μM
Compound: 8
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Cytotoxicity against human WI-38 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay
Cytotoxicity against human WI-38 cells assessed as cell growth inhibition measured after 24 to 72 hrs by MTT assay
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[PMID: 33586438] |
Physalin F is a seco-steroid from Physalis angulate L.[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 57423-71-9
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Appearance Solid
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Peso molecular 526.53
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Fòrmula C28H30O10
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Color White to off-white
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SMILES
C[C@@]([C@@](OC1=O)([H])C2)(OC3=O)[C@@]([C@@]3(CC[C@@]4([H])[C@@]5([H])C[C@@H](O6)[C@]6(CC=C7)[C@@]4(C7=O)C)O)(O[C@@]58C9=O)[C@]9([H])[C@]2([C@@]1([H])CO8)C
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvente y solubilidad
DMSO : 100 mg/mL (189.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.11 mg/mL (2.11 mM); Clear solution
This protocol yields a clear solution of ≥ 1.11 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (11.1 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8992 mL | 9.4961 mL | 18.9923 mL | 47.4807 mL |
| 5 mM | 0.3798 mL | 1.8992 mL | 3.7985 mL | 9.4961 mL | |
| 10 mM | 0.1899 mL | 0.9496 mL | 1.8992 mL | 4.7481 mL | |
| 15 mM | 0.1266 mL | 0.6331 mL | 1.2662 mL | 3.1654 mL | |
| 20 mM | 0.0950 mL | 0.4748 mL | 0.9496 mL | 2.3740 mL | |
| 25 mM | 0.0760 mL | 0.3798 mL | 0.7597 mL | 1.8992 mL | |
| 30 mM | 0.0633 mL | 0.3165 mL | 0.6331 mL | 1.5827 mL | |
| 40 mM | 0.0475 mL | 0.2374 mL | 0.4748 mL | 1.1870 mL | |
| 50 mM | 0.0380 mL | 0.1899 mL | 0.3798 mL | 0.9496 mL | |
| 60 mM | 0.0317 mL | 0.1583 mL | 0.3165 mL | 0.7913 mL | |
| 80 mM | 0.0237 mL | 0.1187 mL | 0.2374 mL | 0.5935 mL | |
| 100 mM | 0.0190 mL | 0.0950 mL | 0.1899 mL | 0.4748 mL |
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