Based on 1 publication(s) in Google Scholar
Purmorphamine (GMP) is Purmorphamine (HY-15108) produced by using GMP guidelines. GMP small molecules work appropriately as an auxiliary reagent for cell therapy manufacture. Purmorphamine is a smoothened/Smo receptor agonist with an EC50 of 1 μM.
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- No. CAS: 483367-10-8
- Fòrmula: C31H32N6O2
- Peso molecular:520.62
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Publications Citing Use of MedChemExpress (MCE) Purmorphamine (GMP)
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Actividad biológica
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| C3H 10T1/2 | EC50 |
0.8 μM
Compound: Purmorph
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Activity at Smo in mouse C3H10T1/2 cells assessed as induction of cell differentiation into osteoblast incubated for 6 days by alkaline phosphatase assay
Activity at Smo in mouse C3H10T1/2 cells assessed as induction of cell differentiation into osteoblast incubated for 6 days by alkaline phosphatase assay
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[PMID: 27429255] |
| C3H 10T1/2 | EC50 |
1 μM
Compound: Purmorphamine
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Induction of osteogenesis in mouse C3H10T1/2 cells assessed as induction of osteoblast specific marker alkaline phosphatase by immunofluorescence method
Induction of osteogenesis in mouse C3H10T1/2 cells assessed as induction of osteoblast specific marker alkaline phosphatase by immunofluorescence method
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[PMID: 16408003] |
| HEK-293T | IC50 |
1.5 μM
Compound: 3
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Inhibition of BODIPY-cyclopamine binding to Smo expressed in HEK293T cells after 1 hr by fluorescence microscopy
Inhibition of BODIPY-cyclopamine binding to Smo expressed in HEK293T cells after 1 hr by fluorescence microscopy
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[PMID: 16408088] |
| Shh Light II | EC50 |
1 μM
Compound: 3
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Activation of Shh in mouse Shh Light2 cells after 30 hrs by luciferase reporter gene assay
Activation of Shh in mouse Shh Light2 cells after 30 hrs by luciferase reporter gene assay
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[PMID: 16408088] |
Purmorphamine (GMP) (1.5 μM, 1 week) can generate embryoid bodies (EBs) from human iPS cells (hiPS2) and hESCs (HSF1)[1].
Purmorphamine (GMP) (1.5 μM) converts hPSCs to NKX2-1 positive MGE cells[2].
Purmorphamine (GMP) (2 μM, day 28-35) promotes ventralization of the brain spheroids in differentiation process from hiPSCs[3].
Purmorphamine (GMP) (1 μM) together with retinoic acid (RA, 2 μM) promotes hiPSC differentiation into functional neural tissue, indicated by the expression of neuronal marker β-tubulin III (βT-III)[4].
Purmorphamine (GMP) (day5-7) induces hESCs differentiation into dopaminergic (DA) neurons[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 483367-10-8
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Appearance Solid
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Peso molecular 520.62
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Fòrmula C31H32N6O2
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Color Off-white to pale purple
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SMILES
N1(C2=CC=C(NC3=NC(OC4=CC=CC5=C4C=CC=C5)=NC6=C3N=CN6C7CCCCC7)C=C2)CCOCC1
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (1)
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Journal Impact Factor
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Most Recent
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Life Sci
Cryopreserved clinical-grade human embryonic stem cell-derived dopaminergic progenitors function in Parkinson's disease models. [Abstract]2023 Sep 15:329:121990. PMID: 37524159
Pureza y Documentación
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Ficha de datos (278 KB)
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SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Karumbayaram S, et al. Directed differentiation of human-induced pluripotent stem cells generates active motor neurons. Stem Cells. 2009 Apr;27(4):806-11. [Content Brief]
[2]. Hu Y, et al. Directed differentiation of basal forebrain cholinergic neurons from human pluripotent stem cells. J Neurosci Methods. 2016 Jun 15;266:42-9. [Content Brief]
[3]. Hua T, et al. Phenotypic, metabolic, and biogenesis properties of human stem cell-derived cerebellar spheroids. Sci Rep. 2022 Jul 27;12(1):12880. [Content Brief]
[4]. Sharma R, et al. 3D Bioprinting Pluripotent Stem Cell Derived Neural Tissues Using a Novel Fibrin Bioink Containing Drug Releasing Microspheres. Front Bioeng Biotechnol. 2020 Feb 11;8:57. [Content Brief]
[5]. Sundberg M, et al. Improved cell therapy protocols for Parkinson's disease based on differentiation efficiency and safety of hESC-, hiPSC-, and non-human primate iPSC-derived dopaminergic neurons. Stem Cells. 2013 Aug;31(8):1548-62. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purmorphamine (GMP) Related Classifications
HY-15108G Related Classifications
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