SKOG102
Based on 1 Customer Validation
SKOG102 is a potent OLIG2 inhibitor that is directly engaging OLIG2 and interferes with the ability of OLIG2 to bind DNA. SKOG102 can be used for the research of glioblastoma (GBM).
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.09%
- No. CAS: 21062-28-2
- Fòrmula: C19H25Cl2N7
- Peso molecular:422.35
-
Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Actividad biológica
-
Chemical Information
-
Solvente y solubilidad
- Pureza y Documentación
- Referencias
-
Help & FAQs
Actividad biológica
Descripciòn
IC50 & Target
OLIG2[1]
Cellular Effect
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| PC-12 | IC50 |
1.1 μM
Compound: 18, TKO-10-20
|
Inhibition of nicotinic cholinergic receptor in rat PC12 cells assessed as inhibition of nicotine-stimulated [3H]-norepinephrine release after 30 mins by liquid scintillation counting analysis
Inhibition of nicotinic cholinergic receptor in rat PC12 cells assessed as inhibition of nicotine-stimulated [3H]-norepinephrine release after 30 mins by liquid scintillation counting analysis
|
[PMID: 23916146] |
In Vitro
SKOG102 (0.1-5 μM; 72 h) inhibits human GBM4 and GBM8 cells grown as neurospheres, with IC50s of 10.66 and 1.536 μM, respectively[1].
SKOG102 (0.5-5 μM; 12 h) increases p21 RNA levels and decreases OMG RNA levels in GBM4 cells[1].
SKOG102 (5 μM; 20 h) suppresses OLIG2-DNA binding, and decreases the serine phosphorylated OLIG2 and total OLIG2 protein levels in GBM8 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
SKOG102 (5 mg/kg; i.p.) accumulates in the tumor even over a relatively brief time period and the plasma and brain concentrations are almost identical at 4 hours after injection in mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:NSG mice were inoculated in each flank with GBM4 cells[1]
-
Dosage:10, 15, and 20 mg/kg; dissolved in solutol:PEG400:water (20%:40%:40%)
-
Administration:Intraperitoneal injection; first 2 weeks (from day 33 to 46) 10 mg/kg, third week (from day 47 to 54) 15 mg/kg, fourth week (from 55 to 66) first 3 days 20 mg/kg, 2 days break, then alternate days from day 60 to 66
-
Result:Significantly attenuated tumor growth.
Chemical Information
-
No. CAS 21062-28-2
-
Appearance Solid
-
Peso molecular 422.35
-
Fòrmula C19H25Cl2N7
-
Color White to off-white
-
SMILES
N=C(NC1=NC(C)=CC(NC2CN(CC)CCC2)=N1)NC3=CC=C(Cl)C(Cl)=C3
-
Envío
Room temperature in continental US; may vary elsewhere.
-
Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (236.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 6.25 mg/mL (14.80 mM); Clear solution
This protocol yields a clear solution of ≥ 6.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.92 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
-
Ficha de datos (270 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3677 mL | 11.8385 mL | 23.6770 mL | 59.1926 mL |
| 5 mM | 0.4735 mL | 2.3677 mL | 4.7354 mL | 11.8385 mL | |
| 10 mM | 0.2368 mL | 1.1839 mL | 2.3677 mL | 5.9193 mL | |
| 15 mM | 0.1578 mL | 0.7892 mL | 1.5785 mL | 3.9462 mL | |
| 20 mM | 0.1184 mL | 0.5919 mL | 1.1839 mL | 2.9596 mL | |
| 25 mM | 0.0947 mL | 0.4735 mL | 0.9471 mL | 2.3677 mL | |
| 30 mM | 0.0789 mL | 0.3946 mL | 0.7892 mL | 1.9731 mL | |
| 40 mM | 0.0592 mL | 0.2960 mL | 0.5919 mL | 1.4798 mL | |
| 50 mM | 0.0474 mL | 0.2368 mL | 0.4735 mL | 1.1839 mL | |
| 60 mM | 0.0395 mL | 0.1973 mL | 0.3946 mL | 0.9865 mL | |
| 80 mM | 0.0296 mL | 0.1480 mL | 0.2960 mL | 0.7399 mL | |
| 100 mM | 0.0237 mL | 0.1184 mL | 0.2368 mL | 0.5919 mL |
SKOG102 Related Classifications
HY-125427 Related Classifications
Powered by Bioz