SR12418
Based on 1 Customer Validation
SR12418 is a REV-ERB-specific synthetic ligand with IC50s of 68 nM and 119 nM for REV-ERBα and REV-ERBβ, respectively. SR12418 can be used in experimental autoimmune encephalomyelitis (EAE) and colitis research.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.94%
- No. CAS: 1801185-08-9
- Fòrmula: C31H30FNO3
- Peso molecular:483.57
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
IC50: 68 nM (REV-ERBα) and 119 nM (REV-ERBβ)[1]
SR12418 (5 μM; 96 h) inhibits the growth of TH17 cells[1].
SR12418 (5 and 10 μM; 4 d) inhibits cell differentiation under TH17 polarizing conditions[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:TH17 cells
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Concentration:5 μM
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Incubation Time:96 hours
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Result:Repressed TH17-mediated gene expression and Nfil3.
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Cell Line:Mouse CD4+ T cells
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Concentration:5 and 10 μM
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Incubation Time:4 days
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Result:Inhibited TH17 cell differentiation in a dose-dependent manner.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 mice inducing experimental autoimmune encephalomyelitis (EAE)[1]
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Dosage:50 mg/kg
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Administration:Intraperitoneal injection; 50 mg/kg; twice a day
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Result:Diminished the incidence of disease with approximately 20% mice developing disease. Showed no overt signs of toxicity.
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Animal Model:PLP139-151-induced relapsing-remitting EAE (R-EAE) in SJL/J mice[1]
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Dosage:50 mg/kg
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Administration:Intraperitoneal injection; 50 mg/kg; twice a day; begin at day 18 post-immunization
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Result:Resulted in a significant reduction in the relapse severity compared to the vehicle control. Showed a significant decrease in the frequency and number of CD4+ and CD8+ effector cells (CD44hi).
Chemical Information
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No. CAS 1801185-08-9
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Appearance Solid
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Peso molecular 483.57
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Fòrmula C31H30FNO3
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Color White to off-white
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SMILES
O=C(N1CC2=C(C=C(F)C=C2)C[C@H]1COC3=CC=C(OC(C)(C)C)C=C3)C4=C5C=CC=CC5=CC=C4
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
DMSO : 100 mg/mL (206.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (5.17 mM); Clear solution; Need ultrasonic
This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (278 KB)
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SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.0680 mL | 10.3398 mL | 20.6795 mL | 51.6988 mL |
| 5 mM | 0.4136 mL | 2.0680 mL | 4.1359 mL | 10.3398 mL | |
| 10 mM | 0.2068 mL | 1.0340 mL | 2.0680 mL | 5.1699 mL | |
| 15 mM | 0.1379 mL | 0.6893 mL | 1.3786 mL | 3.4466 mL | |
| 20 mM | 0.1034 mL | 0.5170 mL | 1.0340 mL | 2.5849 mL | |
| 25 mM | 0.0827 mL | 0.4136 mL | 0.8272 mL | 2.0680 mL | |
| 30 mM | 0.0689 mL | 0.3447 mL | 0.6893 mL | 1.7233 mL | |
| 40 mM | 0.0517 mL | 0.2585 mL | 0.5170 mL | 1.2925 mL | |
| 50 mM | 0.0414 mL | 0.2068 mL | 0.4136 mL | 1.0340 mL | |
| 60 mM | 0.0345 mL | 0.1723 mL | 0.3447 mL | 0.8616 mL | |
| 80 mM | 0.0258 mL | 0.1292 mL | 0.2585 mL | 0.6462 mL | |
| 100 mM | 0.0207 mL | 0.1034 mL | 0.2068 mL | 0.5170 mL |
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