Win 18446
Based on 3 publication(s) in Google Scholar
Win 18446 is an orally active testes-specific enzyme ALDH1a2 inhibitor, with an IC50 of 0.3 μM. Win 18446 reversibly inhibits spermatogenesis in many species and inhibits Retinoic acid (HY-14649) biosynthesis from Retinol (HY-B1342) within the testes.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 98.94%
- No. CAS: 1477-57-2
- Fòrmula: C12H20Cl4N2O2
- Peso molecular:366.11
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Almacenamiento:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
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Chemical Information
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Solvente y solubilidad
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Publications Citing Use of MedChemExpress (MCE) Win 18446
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Actividad biológica
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ALDH1 |
WIN 18446 (1 μM, 24 h) is able to suppress Stra8 expression in whole-testes cultures that contained both germ and somatic cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Testes were collected from 2-dpp mice.
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Concentration:1 μM.
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Incubation Time:24 h.
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Result:Results in a VAD-like phenotype in the testis.
Alone or in combination with ROL significantly reduced Stra8 expression.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Six-month-old male New Zealand white rabbits[1].
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Dosage:200 mg/kg.
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Administration:Orally for 4, 8, and 16 weeks.
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Result:Resulted in marked suppression of testicular weight over time.
Dramatically suppressed spermatogenesis.
Significantly reduces intratesticular concentrations of retinoic acid and Stra8 expression and very severely suppresses spermatogenesis and fertility.
Chemical Information
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No. CAS 1477-57-2
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Appearance Solid
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Peso molecular 366.11
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Fòrmula C12H20Cl4N2O2
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Color White to light brown
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SMILES
O=C(NCCCCCCCCNC(C(Cl)Cl)=O)C(Cl)Cl
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Publications (3)
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Journal Impact Factor
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Most Recent
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Sci Adv
Histone demethylase LSD1 is critical for endochondral ossification during bone fracture healing. [Abstract]2020 Nov 4;6(45):eaaz1410. PMID: 33148658 -
Anal Chem
Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]2025 Jun 3;97(21):11099-11109. PMID: 40401576 -
J Biol Chem
A cell-based assay for retinaldehyde dehydrogenase activity: Retinoid quantification as an alternative to current fluorescence-based approaches. [Abstract]2026 Jan 29;302(3):111211. PMID: 41617028
Win 18446 purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2026 Jan 29;302(3):111211. [Abstract]
Win18446 (Win446, 1 μM). The ATRA content of each fraction is presented as a portion of total levels. Data presented as mean ± S.E. from 5 separate experiments. H–J, mutuDC1940 cells were treated with 9cRA and assayed with 10 μM RAL.
Win 18446 purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2026 Jan 29;302(3):111211. [Abstract]
ROL abundance analysis was performed on mutuDC1940 cells treated with 9cRA, 9cRA + 1 μM Win 18446 (Win446), or 9cRA + 10 μM DEAB.
Win 18446 purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2026 Jan 29;302(3):111211. [Abstract]
4OHRA abundance analysis was performed on mutuDC1940 cells treated with 9cRA, 9cRA + 1 μM Win 18446 (Win446), or 9cRA + 10 μM DEAB.
Win 18446 purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2026 Jan 29;302(3):111211. [Abstract]
mutuDC1940 cells were treated for 20 h with or without 1 μM 9cRA, then subjected to the AldeRed retention assay in the presence or absence of 1 μM Win446 or 10 μM DEAB. The AldeRed retention assay was performed with 0.61 μg/ml AldeRed at either low (0.2 × 106 cells/ml) or high (1 × 106 cells/ml) cell density.
Win 18446 purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2026 Jan 29;302(3):111211. [Abstract]
mutuDC1940 cells were treated as Win18446 (Win446, 1 μM), except Medium B was replaced with the assay buffer from the AldeRed assay kit (Merck, catalogue #SCR150) for the ATRA production assay.
Solvente y solubilidad
DMSO : 100 mg/mL (273.14 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.68 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (271 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. John K Amory, et al. Suppression of spermatogenesis by bisdichloroacetyldiamines is mediated by inhibition of testicular retinoic acid biosynthesis. J Androl. Jan-Feb 2011;32(1):111-9. [Content Brief]
[2]. Cathryn A Hogarth, et al. Suppression of Stra8 expression in the mouse gonad by WIN 18,446. Biol Reprod. 2011 May;84(5):957-65. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7314 mL | 13.6571 mL | 27.3142 mL | 68.2855 mL |
| 5 mM | 0.5463 mL | 2.7314 mL | 5.4628 mL | 13.6571 mL | |
| 10 mM | 0.2731 mL | 1.3657 mL | 2.7314 mL | 6.8285 mL | |
| 15 mM | 0.1821 mL | 0.9105 mL | 1.8209 mL | 4.5524 mL | |
| 20 mM | 0.1366 mL | 0.6829 mL | 1.3657 mL | 3.4143 mL | |
| 25 mM | 0.1093 mL | 0.5463 mL | 1.0926 mL | 2.7314 mL | |
| 30 mM | 0.0910 mL | 0.4552 mL | 0.9105 mL | 2.2762 mL | |
| 40 mM | 0.0683 mL | 0.3414 mL | 0.6829 mL | 1.7071 mL | |
| 50 mM | 0.0546 mL | 0.2731 mL | 0.5463 mL | 1.3657 mL | |
| 60 mM | 0.0455 mL | 0.2276 mL | 0.4552 mL | 1.1381 mL | |
| 80 mM | 0.0341 mL | 0.1707 mL | 0.3414 mL | 0.8536 mL | |
| 100 mM | 0.0273 mL | 0.1366 mL | 0.2731 mL | 0.6829 mL |
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