RNF20 - ring finger protein 20 Gene

Also Known as BRE1; BRE1A; hBRE1

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 56254

About RNF20

Cytogenetic location: 9q31.1 Genomic coordinates (GRCh38): 9:101,533,853-101,563,344 (from NCBI)

This gene has 6 transcripts (splice variants), 208 orthologues and 1 paralogue. Ubiquitous expression in testis (RPKM 16.7), brain (RPKM 13.7) and 25 other tissues.

Summary

The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. The protein encoded by this human gene is an E3 ubiquitin Ligase that regulates chromosome structure by monoubiquitinating histone H2B. This protein acts as a putative tumor suppressor and positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. In contrast, this protein also suppresses the expression of several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor. This gene selectively suppresses the expression of some genes by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS). [provided by RefSeq, Feb 2012]

RNF20 Products (1)

mRNA	Protein	Name
NM_019592.7	NP_062538.5	E3 ubiquitin-protein ligase BRE1A

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables histone H2B C-terminal K residue ubiquitin ligase activity	IMP IMP: Inferred from mutant phenotype	16337599	GOA
enables histone binding	IDA IDA: Inferred from direct assay	16337599	GOA
enables identical protein binding	IPI IPI: Inferred from physical interaction	32296183	GOA
enables mRNA 3'-UTR binding	IDA IDA: Inferred from direct assay	19575011	GOA
enables p53 binding	IDA IDA: Inferred from direct assay	16337599	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	16307923	GOA
enables transcription coactivator activity	IDA IDA: Inferred from direct assay	16337599	GOA
enables ubiquitin protein ligase binding	IPI IPI: Inferred from physical interaction	16307923	GOA
contributes to ubiquitin-protein transferase activity	IDA IDA: Inferred from direct assay	16307923	GOA
enables ubiquitin-protein transferase activity	IDA IDA: Inferred from direct assay	19037095	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in negative regulation of cell migration	IDA IDA: Inferred from direct assay	18832071	GOA
involved in positive regulation of DNA-templated transcription	IMP IMP: Inferred from mutant phenotype	16713563	GOA
involved in positive regulation of transcription by RNA polymerase II	IDA IDA: Inferred from direct assay	16307923	GOA
involved in protein polyubiquitination	IDA IDA: Inferred from direct assay	19037095	GOA
involved in regulation of DNA-templated transcription	IMP IMP: Inferred from mutant phenotype	18832071	GOA
NOT involved in regulation of mitotic cell cycle	IMP IMP: Inferred from mutant phenotype	19575011	GOA
involved in ubiquitin-dependent protein catabolic process	IMP IMP: Inferred from mutant phenotype	19037095	GOA

Cellular Component GO Annotation	Evidence	References	Source
part of HULC complex	IDA IDA: Inferred from direct assay	19410543	GOA
located in nucleolus	IDA IDA: Inferred from direct assay	19037095	GOA
located in nucleus	IDA IDA: Inferred from direct assay	16307923	GOA
part of ubiquitin ligase complex	IDA IDA: Inferred from direct assay	16307923	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

RNF20 Protein Structure

zf-C3HC4

0
200
400
600
800
975 a.a.

Protein Preferred Names	Protein Names
E3 ubiquitin-protein ligase BRE1A	BRE1 E3 ubiquitin ligase homolog

RNF20 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	RNF20	Q5VTR2	AMOTL2	Homo sapiens	Q9Y2J4-4	Y2H Array	25416956
Intra	RNF20	Q5VTR2	CHEK2	Homo sapiens	O96017	Anti Tag CoIP	25640309
Intra	RNF20	Q5VTR2	RNF40	Homo sapiens	O75150	Anti Tag CoIP	19410543
Intra	RNF20	Q5VTR2	RNF40	Homo sapiens	O75150	Chromatography	19410543
Intra	RNF20	Q5VTR2	RNF40	Homo sapiens	O75150	GMS	16307923
Intra	RNF20	Q5VTR2	TACC3	Homo sapiens	Q9Y6A5	Y2H Prey Pooling	32296183
Intra	RNF20	Q5VTR2	TACC3	Homo sapiens	Q9Y6A5	Validated Y2H	32296183
Intra	RNF20	Q5VTR2	TACC3	Homo sapiens	Q9Y6A5	Y2H Array	32296183
Intra	RNF20	Q5VTR2	UBE2E1	Homo sapiens	P51965	Pull Down	16307923
Intra	RNF20	Q5VTR2	HTT	Homo sapiens	P42858	Y2H Prey Pooling	23275563
Intra	RNF20	Q5VTR2	HTT	Homo sapiens	P42858	Y2H	17500595
Intra	RNF20	Q5VTR2	COPS5	Homo sapiens	Q92905	Biochemical	19214193
Intra	RNF20	Q5VTR2	USHBP1	Homo sapiens	Q8N6Y0	Validated Y2H	25416956
Intra	RNF20	Q5VTR2	USHBP1	Homo sapiens	Q8N6Y0	Y2H Array	25416956
Intra	RNF20	Q5VTR2	AMOTL2	Homo sapiens	Q9Y2J4	Y2H Array	32296183
Intra	RNF20	Q5VTR2	AMOTL2	Homo sapiens	Q9Y2J4	Validated Y2H	32296183
Intra	RNF20	Q5VTR2	AMOTL2	Homo sapiens	Q9Y2J4	Y2H Prey Pooling	32296183
Intra	RNF20	Q5VTR2	AIRIM	Homo sapiens	Q9NX04	Validated Y2H	32296183
Intra	RNF20	Q5VTR2	CDC73	Homo sapiens	Q6P1J9	Validated Y2H	32296183

Cross: Cross-species interaction Intra: Intraspecies interaction

RNF20 Antibodies

Cat. No.	Product Name	Application	Reactivity
HY-P83135	RNF20 Antibody (YA2880)	WB, ICC/IF, FC, IP	Human, Mouse

Related Diseases

Diseases	Alias
Testicular Germ Cell Cancer	Germ Cell Tumor Of Testis Testicular Germ Cell Tumor Testicular Germ Cell Neoplasm Testicular Malignant Germ Cell Tumor

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (3)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-RS12075	RNF20 Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS20095	Rnf20 Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS26598	Rnf20 Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Macaca mulatta	RNF20	VGNC	VGNC:76704
Felis catus	RNF20	VGNC	VGNC:64689
Canis familiaris	RNF20	VGNC	VGNC:45653
Mus musculus	RNF20	MGD	MGI:1925927
Rattus norvegicus	RNF20	RGD	RGD:1311936
Bos taurus	RNF20	VGNC	VGNC:34047
Others	RNF20	NCBI