Glycerol Monoleate

Name: Glycerol Monoleate
Brand: MedChemExpress
SKU: HY-N1446A
Rating: 5.0 (60 reviews)

Cat. No.: HY-N1446A Purity: 95.21%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Glycerol Monoleate is a non-toxic, biodegradable, biocompatible, lipophilic glycerol fatty acid ester. Glycerol Monoleate exhibits hemolytic properties. Glycerol Monoleate is combined with bile salts for use as an emulsifier and absorption enhancer. Glycerol Monoleate can be applied in drug delivery systems and in vitro siRNA delivery.

For research use only. We do not sell to patients.

CAS No. : 25496-72-4

Size	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Liquid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 60 publication(s) in Google Scholar

Other Forms of Glycerol Monoleate:

Oleic acid In-stock

60 Publications Citing Use of MCE Glycerol Monoleate

Cell Proliferation/Viability Assay

: Glycerol Monoleate purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Feb 3:80:103528. [Abstract]; OVCAR8 and A2780 cells were cultured in fatty acid-free media supplemented with 50 μM AA (arachidonic acid), 50 μM OA (oleic acid), 50 μM EPA (eicosapentaenoic acid), 50 μM PA (Palmitic acid), and 50 μM MA (myristic acid) with the treatment of 10 μM niraparib for 72 h, and the cell viability was determined.

: Glycerol Monoleate purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Aug 12;42(8):1386-1400.e8. [Abstract]; Oleic acid (50 μM; 1-4 d) significantly promotes viability in CRC cells (HCT116, HT-29, DLD-1).

: Glycerol Monoleate purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2024 Aug 12;42(8):1386-1400.e8. [Abstract]; Oleic acid (50 μM) significantly promotes the proliferation of colorectal cancer (CRC) cells (HCT116, HT-29).

: Glycerol Monoleate purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2024 Jan;13(1):e12401. [Abstract]; Oleic acid (500-1000 μM; 24 h) significantly inhibits the activity of ARPE-19 cells.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

Glycerol Monoleate (7.5-50 wt % DDAB or DOTAP; N/P ratios 1,4; 40 min electrophoresis at 100 V)-based nanocarrier complexes with DOTAP exhibit stronger siRNA binding than equivalent DDAB-containing complexes, with complete siRNA binding observed for 15-50 wt % DOTAP at N/P=4^[2].
Glycerol Monoleate (7.5-50 wt % DDAB or DOTAP; N/P ratios 1,4; 72 h cell treatment; 4 h Alamar Blue incubation)-based siRNA nanocarrier complexes with DDAB or DOTAP show no significant cytotoxicity at N/P=1 in CHO-GFP and HEK293 cells; at N/P=4, DDAB-containing complexes show cytotoxicity at 7.5%, 15%, and 50% loading, while only 7.5% DOTAP-containing complexes show cytotoxicity^[2].
Glycerol Monoleate (7.5-50 wt % DDAB or DOTAP; N/P ratios 1,4; 4 h incubation with complexes in OPTI-MEM; 72 h incubation in standard growth media)-based siRNA nanocarrier complexes with 7.5% DOTAP at N/P=1 induce 70% GFP gene silencing in CHO-GFP cells, showing higher silencing efficiency with decreasing cationic lipid loading (increasing GMO content) and lower charge ratios^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Glycerol monooleate-based formulations enhance the oral bioavailability of cinnarizine in Rattus norvegicus^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

374.56

Formula

C₂₁H₄₂O₅

CAS No.

25496-72-4

Appearance

Liquid (Density: 0.96 g/cm³)

Color

Colorless to light yellow

SMILES

OC(CCCCCCC/C=C\CCCCCCCC)=O.OCC(O)CO

Structure Classification

Ketones, Aldehydes, Acids

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 200 mg/mL (533.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.6698 mL	13.3490 mL	26.6980 mL
5 mM	0.5340 mL	2.6698 mL	5.3396 mL
10 mM	0.2670 mL	1.3349 mL	2.6698 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 95.21%

Select Batch:

Data Sheet (268 KB) SDS (393 KB)

COA (239 KB) HNMR (261 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Milak S, et al. Glycerol monooleate liquid crystalline phases used in drug delivery systems. Int J Pharm. 2015;478(2):569-587. [Content Brief]

[2]. Zhen G, et al. Glycerol monooleate-based nanocarriers for siRNA delivery in vitro. Mol Pharm. 2012;9(9):2450-2457. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.6698 mL	13.3490 mL	26.6980 mL	66.7450 mL
	5 mM	0.5340 mL	2.6698 mL	5.3396 mL	13.3490 mL
	10 mM	0.2670 mL	1.3349 mL	2.6698 mL	6.6745 mL
	15 mM	0.1780 mL	0.8899 mL	1.7799 mL	4.4497 mL
	20 mM	0.1335 mL	0.6674 mL	1.3349 mL	3.3372 mL
	25 mM	0.1068 mL	0.5340 mL	1.0679 mL	2.6698 mL
	30 mM	0.0890 mL	0.4450 mL	0.8899 mL	2.2248 mL
	40 mM	0.0667 mL	0.3337 mL	0.6674 mL	1.6686 mL
	50 mM	0.0534 mL	0.2670 mL	0.5340 mL	1.3349 mL
	60 mM	0.0445 mL	0.2225 mL	0.4450 mL	1.1124 mL
	80 mM	0.0334 mL	0.1669 mL	0.3337 mL	0.8343 mL
	100 mM	0.0267 mL	0.1335 mL	0.2670 mL	0.6674 mL