GPR35 agonist 5

Cat. No.: HY-149190 Purity: 98.86%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

GPR35 agonist 5 (3,5-dinitro-bisphenol A; compound 6) is a weak GPR35 agonist. GPR35 agonist 5 arrests CHO-S cells at the G1/Gophase.

For research use only. We do not sell to patients.

GPR35 agonist 5 Chemical Structure

CAS No. : 2226201-24-5

Size	Price	Stock	Quantity
5 mg	USD 435	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 700	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 1400	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 2250	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 3560	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Description

GPR35 agonist 5 (3,5-dinitro-bisphenol A; compound 6) is a weak GPR35 agonist. GPR35 agonist 5 arrests CHO-S cells at the G1/Gophase^[1].

Molecular Weight

318.28

Formula

C₁₅H₁₄N₂O₆

CAS No.

2226201-24-5

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=[N+](C1=CC(C(C)(C)C2=CC=C(C=C2)O)=CC([N+]([O-])=O)=C1O)[O-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (314.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1419 mL	15.7094 mL	31.4189 mL
5 mM	0.6284 mL	3.1419 mL	6.2838 mL
10 mM	0.3142 mL	1.5709 mL	3.1419 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (7.85 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (7.85 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.86%

Select Batch:

Data Sheet (275 KB) SDS (251 KB)

COA (254 KB) HNMR (135 KB) RP-HPLC (139 KB) LCMS (359 KB)

Handling Instructions (2659 KB)

References

[1]. Jinlin Peng, et al. Chemical Identity and Mechanism of Action and Formation of a Cell Growth Inhibitory Compound from Polycarbonate Flasks. Anal Chem. 2018 Apr 3;90(7):4603-4610. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1419 mL	15.7094 mL	31.4189 mL	78.5472 mL
	5 mM	0.6284 mL	3.1419 mL	6.2838 mL	15.7094 mL
	10 mM	0.3142 mL	1.5709 mL	3.1419 mL	7.8547 mL
	15 mM	0.2095 mL	1.0473 mL	2.0946 mL	5.2365 mL
	20 mM	0.1571 mL	0.7855 mL	1.5709 mL	3.9274 mL
	25 mM	0.1257 mL	0.6284 mL	1.2568 mL	3.1419 mL
	30 mM	0.1047 mL	0.5236 mL	1.0473 mL	2.6182 mL
	40 mM	0.0785 mL	0.3927 mL	0.7855 mL	1.9637 mL
	50 mM	0.0628 mL	0.3142 mL	0.6284 mL	1.5709 mL
	60 mM	0.0524 mL	0.2618 mL	0.5236 mL	1.3091 mL
	80 mM	0.0393 mL	0.1964 mL	0.3927 mL	0.9818 mL
	100 mM	0.0314 mL	0.1571 mL	0.3142 mL	0.7855 mL

GPR35 agonist 5 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

GPR35 agonist 52226201-24-5GPR35G Protein-Coupled Receptor 35GPR35 agonistInhibitorinhibitorinhibit

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