HS38 

HS38 is a potent, selective, and ATP-competitive inhibitor of death-associated protein kinase 1 (DAPK1) and zipper-interacting protein kinase (ZIPK, also called DAPK3), with K_ds of 300 nM and 280 nM, respectively. HS38 is also a PIM3 inhibitor with an IC₅₀ of 200 nM. HS38 can be used for the research of smooth muscle related disorders^[1].

Kd: 300 nM (DAPK1), 79 nM (DAPK2), 280 nM (ZIPK), IC50: 200 nM (DAPK1), 200 nM (PIM3)^[1]

HS38 displays high affinity toward DAPK2, with a K_d of 79 nM. DAPK2 is not implicated in smooth muscle contractility^[1].
HS38 significantly reduces relative RLC20 phosphorylation in both the basal and sphingosine 1-phosphate (S1P) activated states in human aortic SM cells^[1].
HS38 reduces contractile forces generated by intact mouse aorta in aortic tissue^[1].
HS38 reduces the contractile force, RLC20 phosphorylation, and MYPT1 phosphorylation in Ca²⁺-sensitized rabbit ileum^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

349.80

C₁₄H₁₂ClN₅O₂S

1030203-81-6

Solid

White to off-white

CC(SC1=NC(N(C2=CC=CC(Cl)=C2)N=C3)=C3C(N1)=O)C(N)=O

Room temperature in continental US; may vary elsewhere.

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. David A Carlson, et al. Fluorescence Linked Enzyme Chemoproteomic Strategy for Discovery of a Potent and Selective DAPK1 and ZIPK Inhibitor. ACS Chem Biol. 2013 Dec 20; 8(12): 2715–2723.  [Content Brief]