Cat. No.: HY-N0448

10-Gingerol

4 Cited Publications

Customer Review

Based on 4 publication(s) in Google Scholar

10-Gingerol is an AMPK agonist, which is found in the ginger oleoresin from fresh rhizome with anti-inflammatory, antioxidant and anti-proliferative activities. 10-Gingerol suppresses neointimal hyperplasia and inhibits vascular smooth muscle cell proliferation. 10-Gingerol exhibits substantial scavenging activities with an IC₅₀ value of 10.47 μM against DPPH radical, an IC₅₀ value of 1.68 μM against superoxide radical and an IC₅₀ value of 1.35 μM against hydroxyl radical. 10-Gingerol inhibits the proliferation of MDA-MB-231 tumor cell line with an IC₅₀ of 12.1 μM. 10-Gingerol suppresses the proliferation, migration, invasion, and induced apoptosis through targeting the PI3K/Akt signaling pathway in MDA-MB-231/IR cells. 10-Gingerol can be used in research on various common cancers such as ovarian cancer and colon cancer, as well as colitis and neurodegenerative diseases.

For research use only. We do not sell to patients.

Purity: 99.48%
CAS No.: 23513-15-7
Formula: C₂₁H₃₄O₄
Molecular Weight:350.49

Storage:

-20°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

10-Gingerol Chemical Structure

CAS No.: 23513-15-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All AMPK Isoforms :

All Akt Isoforms :

All PI3K Isoforms :

All Interleukin Related Isoforms :

All TNF Receptor Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
RBL-2H3	IC₅₀	111.7 μM Compound: 3	Cytotoxicity against rat RBL2H3 cells after 12 hrs by MTT assay Cytotoxicity against rat RBL2H3 cells after 12 hrs by MTT assay	[PMID: 19271742]

In Vitro

10-Gingerol (1-6 μM, 24 h) inhibits NO production in LPS-stimulated RAW 264.7 cells and LPS-activated PGE2 release^[1].
10-Gingerol (100 μM, 24 h) is significantly effective in inhibiting MDA-MB-231 cell proliferation^[2].
10-Gingerol (6.25-200 μM, 24 h and 48 h) suppresses the proliferation, migration, and invasion of MDA-MB-231/IR cells^[3].
10-Gingerol (0-90 μM, 24 h) induces apoptosis and l suppresses PI3K/Akt signaling in MDA-MB-231/IR cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

RAW 264.7 cells
Concentration:

1-6 μM
Incubation Time:

24 h
Result:

Inhibited nitrite release without causing cell death in RAW 264.7 cells.

Cell Proliferation Assay^[2]

Cell Line:

MDA-MB-231 cells
Concentration:

100 μM
Incubation Time:

24 h
Result:

Was significantly effective in inhibiting MDA-MB-231 cell proliferation at 24 h of incubation in a rate of 75%.

Cell Viability Assay^[3]

Cell Line:

MDA-MB-231/IR cells
Concentration:

6.25-200 μM
Incubation Time:

24 h and 48 h
Result:

Causing 50% growth inhibition (IC₅₀ ) of MDA-MB-231/IR cells at 24 and 48 h incubations were 121.2 and 101.4 µM, respectively.

Western Blot Analysis^[3]

Cell Line:

MDA-MB-231/IR cells
Concentration:

0-90 μM
Incubation Time:

24 h
Result:

Decreased the phosphorylation of p-Akt (Ser473), p-Akt (Thr308), p-PI3Kp85 and p4E-BP1, and activated the expression of some markers of apoptosis including Bax/Bcl-2, cleaved caspase-7, cleaved PARP, and cleaved caspase-3 in MDA-MB-231/IR cells.

In Vivo

10-Gingerol (30 mg/kg, i.p., 7 consecutive days) reverses body weight loss, declines disease activity index (DAI), decreasing myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, as well as restoring SOD activity, reduces the serum TNF-α and IL-1β levels, ameliorates mucosa inflammation and/or defect in rats with DSS-induced acute ulcerative colitis^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Rats with DSS-induced acute ulcerative colitis^[4]
Dosage:

30 mg/kg
Administration:

i.p., 7 consecutive days
Result:

Reversed body weight loss and significantly declined DAI, reduced the serum TNF-α and IL-1β levels, ameliorated mucosa inflammation and/or defect in rats with DSS-induced acute ulcerative colitis in rats with DSS-induced acute ulcerative colitis.

Chemical Information

CAS No. 23513-15-7
Appearance Solid
Molecular Weight 350.49
Formula C₂₁H₃₄O₄
Color White to light yellow
SMILES

CCCCCCCCC[C@H](O)CC(CCC1=CC=C(O)C(OC)=C1)=O
Structure Classification
- Phenols
- Monophenols
- Ketones, Aldehydes, Acids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (4)

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Bioact Mater

Lipidomic analysis of plant-derived extracellular vesicles for guidance of potential anti-cancer therapy. [Abstract]

2024 Dec 10:46:82-96. PMID: 39737211
Food Chem

Conversion of gingerols to shogaols in ginger (Zingiber officinale roscoe) by puffing. [Abstract]

2024 Sep 15:452:139425. PMID: 38744128
Arch Pharm Res

Cartilage extracellular matrix regeneration with 10-gingerol via KEAP1-NRF2-ARE axis for osteoarthritis therapy. [Abstract]

2025 Dec;48(11-12):1460-1481. PMID: 41212495
Pharm Biol

Potential pharmacological quality control markers in the traditional Japanese medicine Hangeshashinto: identifying anti-inflammatory ingredients through a cell-based bioassay and multicomponent analysis. [Abstract]

2025 Dec;63(1):819-836. PMID: 41240012

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (285.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.8531 mL

14.2657 mL

28.5315 mL

5 mM

0.5706 mL

2.8531 mL

5.7063 mL

10 mM

0.2853 mL

1.4266 mL

2.8531 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (7.13 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Dugasani S, et al. Comparative antioxidant and anti-inflammatory effects of [6]-gingerol, [8]-gingerol, [10]-gingerol and [6]-shogaol. J Ethnopharmacol. 2010 Feb 3;127(2):515-20. [Content Brief]

[2]. Almada da Silva J, et al. Purification and differential biological effects of ginger-derived substances on normal and tumor cell lines. J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Aug 15;903:157-62. [Content Brief]

[3]. Ediriweera MK, Moon JY, Nguyen YT, Cho SK. 10-Gingerol Targets Lipid Rafts Associated PI3K/Akt Signaling in Radio-Resistant Triple Negative Breast Cancer Cells. Molecules. 2020;25(14):3164. [Content Brief]

[4]. Zhang F, et al. Therapeutic Effects of 6-Gingerol, 8-Gingerol, and 10-Gingerol on Dextran Sulfate Sodium-Induced Acute Ulcerative Colitis in Rats. Phytother Res. 2017 Sep;31(9):1427-1432. [Content Brief]

[5]. Deng B, et al. 10-Gingerol, a natural AMPK agonist, suppresses neointimal hyperplasia and inhibits vascular smooth muscle cell proliferation. Food Funct. 2022 Mar 21;13(6):3234-3246. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.8531 mL	14.2657 mL	28.5315 mL	71.3287 mL
	5 mM	0.5706 mL	2.8531 mL	5.7063 mL	14.2657 mL
	10 mM	0.2853 mL	1.4266 mL	2.8531 mL	7.1329 mL
	15 mM	0.1902 mL	0.9510 mL	1.9021 mL	4.7552 mL
	20 mM	0.1427 mL	0.7133 mL	1.4266 mL	3.5664 mL
	25 mM	0.1141 mL	0.5706 mL	1.1413 mL	2.8531 mL
	30 mM	0.0951 mL	0.4755 mL	0.9510 mL	2.3776 mL
	40 mM	0.0713 mL	0.3566 mL	0.7133 mL	1.7832 mL
	50 mM	0.0571 mL	0.2853 mL	0.5706 mL	1.4266 mL
	60 mM	0.0476 mL	0.2378 mL	0.4755 mL	1.1888 mL
	80 mM	0.0357 mL	0.1783 mL	0.3566 mL	0.8916 mL
	100 mM	0.0285 mL	0.1427 mL	0.2853 mL	0.7133 mL

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10-Gingerol

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