2-NBDG
Based on 74 publication(s) in Google Scholar
2-NBDG is a fluorescently-labeled deoxyglucose analog that is used primarily to directly monitor glucose uptake by living cells and tissues. It is also used as a topical contrast reagent for the detection of neoplasia. 2-NBDG can be used in real-time confocal, high-resolution, or wide-field fluorescence microscopy as well as in flow cytometry. The probe can be excited by the Argon laser at 488 nm to give the environment-sensitive fluorescence. It has lower photostability than the rhodamine-based fluorescent probes.
For research use only. We do not sell to patients.
- Purity: 99.74%
- CAS No.: 186689-07-6
- Formula: C12H14N4O8
- Molecular Weight:342.26
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 2-NBDG
More- Adv Mater. 2025 Nov 4:e10507. [Abstract]
- Mol Cell. 2025 Jul 3;85(13):2535-2549.e10. [Abstract]
- Cell Death Differ. 2025 Apr 11. [Abstract]
- Acta Pharm Sin B. 2024 Aug;14(8):3576-3590. [Abstract]
- Adv Sci (Weinh). 2026 Apr;13(24):e18902. [Abstract]
- Adv Sci (Weinh). 2025 Mar 24:e2411980. [Abstract]
- Adv Sci (Weinh). 2024 Nov 21:e2411015. [Abstract]
- J Adv Res. 2025 May 9:S2090-1232(25)00299-1. [Abstract]
- J Exp Clin Cancer Res. 2025 Sep 2;44(1):265. [Abstract]
- Redox Biol. 2024 May:71:103112. [Abstract]
- Research (Wash D C). 2024 Mar 11:7:0335. [Abstract]
- Pharmacol Res. 2025 Feb 3:213:107643. [Abstract]
- Proc Natl Acad Sci U S A. 2026 Jul 7;123(27):e2523789123.
- Int J Biol Macromol. 2025 Dec 9:149610. [Abstract]
- Int J Biol Macromol. 2025 Jan 29:301:140488. [Abstract]
- Phytomedicine. 2024 Jul 25:130:155743. [Abstract]
- Phytomedicine. 2024 Jul:129:155592. [Abstract]
- ACS Appl Mater Interfaces. 2025 Apr 1. [Abstract]
- Br J Pharmacol. 2024 Oct;181(20):3886-3907. [Abstract]
- J Transl Med. 2023 Aug 26;21(1):574. [Abstract]
- Oncogene. 2025 May;44(19):1400-1414. [Abstract]
- Microbiol Res. 2025 Sep 24:302:128350. [Abstract]
- Neural Regen Res. 2025 Apr 1;20(4):1178-1191. [Abstract]
- Antioxidants (Basel). 2025 Nov 19;14(11):1381. [Abstract]
- Oncogenesis. 2022 Aug 8;11(1):45. [Abstract]
- Biomater Adv. 2026 Mar:180:214594. [Abstract]
- Mar Drugs. 2025 Nov 24;23(12):450. [Abstract]
- Chem Biol Interact. 2025 Apr 25:411:111450. [Abstract]
- Food Funct. 2021 Mar 21;12(6):2726-2740. [Abstract]
- Mar Drugs. 2020 Sep 22;18(9):485. [Abstract]
- Life Sci. 2026 May 15:393:124343. [Abstract]
- Life Sci. 2024 Nov 4:123215. [Abstract]
- Inflammation. 2023 Aug;46(4):1445-1457. [Abstract]
- Expert Opin Drug Discov. 2025 Aug;20(8):1075-1092. [Abstract]
- Int J Mol Sci. 2020 Jul 25;21(15):5276. [Abstract]
- World J Diabetes. 2023 Sep 15;14(9):1349-1368. [Abstract]
- Lung Cancer. 2024 Feb:188:107449. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2025 Mar 31;1871(5):167814. [Abstract]
- Mol Nutr Food Res. 2025 Mar;69(5):e202400545. [Abstract]
- J Cell Mol Med. 2021 Nov;25(21):9953-9971. [Abstract]
- FASEB J. 2020 Oct;34(10):13548-13560. [Abstract]
- J Cell Mol Med. 2022 Jul;26(13):3783-3796. [Abstract]
- iScience. 2024 Jun 5;27(7):110188. [Abstract]
- Virol Sin. 2025 Nov 21:S1995-820X(25)00161-0. [Abstract]
- J Cell Physiol. 2021 Aug;236(8):5818-5831. [Abstract]
- Ann Clin Transl Neurol. 2026 Mar 24. [Abstract]
- Sci Rep. 2024 Oct 10;14(1):23761. [Abstract]
- Biomedicines. 2024 Oct 3;12(10):2250. [Abstract]
- Cell Signal. 2026 Jul:143:112487. [Abstract]
- Eur J Immunol. 2025 Feb;55(2):e202451440. [Abstract]
- Cell Signal. 2024 Feb:114:110995. [Abstract]
- Heliyon. 2024 May 14;10(10):e31149. [Abstract]
- Biol Trace Elem Res. 2024 Feb;202(2):701-712. [Abstract]
- J Sci Food Agric. 2022 Jan 15;102(1):53-61. [Abstract]
- Arch Med Res. 2025 Aug 18;57(1):103283. [Abstract]
- Toxicol Appl Pharmacol. 2022 Jan 1:434:115798. [Abstract]
- Front Oncol. 2022 Jul 11:12:813852. [Abstract]
- Diabetes Metab Syndr Obes. 2025 Jul 29:18:2573-2584. [Abstract]
- J Diabetes Investig. 2025 Aug;16(8):1382-1397. [Abstract]
- Mol Immunol. 2023 Oct:162:84-94. [Abstract]
- Vet Microbiol. 2025 Apr 11:305:110520. [Abstract]
- Animals (Basel). 2024 Aug 28;14(17):2500. [Abstract]
- Brain Res. 2026 Apr 15:1877-1878:150206. [Abstract]
- Biochem Biophys Res Commun. 2025 Jul 1:768:151938. [Abstract]
- Biochem Biophys Res Commun. 2024 Jul 5:716:150026. [Abstract]
- Biochem Biophys Res Commun. 2021 Oct 28:583:106-113. [Abstract]
- Genes Genomics. 2025 Oct 21. [Abstract]
- Res Sq. 2026 Mar 8.
- SSRN. 6 Oct 2022.
- Oxid Med Cell Longev. 2022 Apr 19;2022:4608914. [Abstract]
- SSRN. 2021 Dec.
- Oxid Med Cell Longev. 2021 Jun 27;2021:5549047. [Abstract]
- Research Square Preprint. 2021 Feb.
- Oxid Med Cell Longev. 2021 Oct 5;2021:8542809. [Abstract]
Customer Validation & Images
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Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Preparation of 2-NBDG working solution
1.1 Preparation of the stock solution
Dissolve 1 mg of 2-NBDG in 2.92 mL of DDH2O to obtain 1 mM of 2-NBDG.
1.2 Preparation of 2-NBDG working solution.
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 10-200 μM of 2-NBDG working solution.
Note: Please adjust the concentration of 2-NBDG working solution according to the actual situation.
Cell staining
2.1 Cell preparation.
For suspension cells: Centrifuge at 1,000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of 2-NBDG working solution, and then incubate at room temperature for 5-60 minutes.
2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.4 Wash twice with PBS, 5 minutes each time.
2.5 Resuspend cells with 1 mL serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.
If test viability, recorded the optical density (O.D.) at 540/570 nm. Cell viability was calculated as a control ratio and plotted against the logarithmic concentration of the drug to calculate IC50.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
542
488
Chemical Information
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CAS No. 186689-07-6
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Appearance Solid
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Molecular Weight 342.26
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Formula C12H14N4O8
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Color Light yellow to orange
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SMILES
O=C[C@H](NC1=CC=C([N+]([O-])=O)C2=NON=C21)[C@H]([C@@H]([C@@H](CO)O)O)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (74)
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Journal Impact Factor
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Most Recent
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Adv Mater
siAkt2-Loaded Nanoparticles Reprogramming Macrophages to M2 Phenotype for Effective Bone Defect Repair. [Abstract]2025 Nov 4:e10507. PMID: 41189396 -
Mol Cell
Glucose-1-phosphate promotes compartmentalization of glycogen with the pentose phosphate pathway in CD8+ memory T cells. [Abstract]2025 Jul 3;85(13):2535-2549.e10. PMID: 40499549 -
Cell Death Differ
Glycogen stores mediated by the p53-GYS1 feedback circuit engenders platinum resistance in ovarian clear cell carcinoma. [Abstract]2025 Apr 11. PMID: 40210982 -
Acta Pharm Sin B
2024 Aug;14(8):3576-3590. PMID: 39220873 -
Adv Sci (Weinh)
Adipocyte-Derived Exosomal miR-5099 Mitigates M1 Macrophage Polarization and Adipose Inflammation via c-Met/NF-κB Axis to Improve Metabolic Health. [Abstract]2026 Apr;13(24):e18902. PMID: 41722058 -
Adv Sci (Weinh)
The Novel Dual GIP and GLP-1 Receptor Agonist Tirzepatide Attenuates Colon Cancer Development by Regulating Glucose Metabolism. [Abstract]2025 Mar 24:e2411980. PMID: 40125821 -
Adv Sci (Weinh)
2024 Nov 21:e2411015. PMID: 39569747 -
J Adv Res
Palmitoylated COX-2Cys555 reprogrammed mitochondrial metabolism in pyroptotic inflammatory injury in patients with post-acute COVID-19 syndrome. [Abstract]2025 May 9:S2090-1232(25)00299-1. PMID: 40349960 -
J Exp Clin Cancer Res
Cancer-associated fibroblast derived CXCL14 drives cisplatin chemoresistance by enhancing nucleotide excision repair in bladder cancer. [Abstract]2025 Sep 2;44(1):265. PMID: 40898244 -
Redox Biol
Enteric coronavirus PDCoV evokes a non-Warburg effect by hijacking pyruvic acid as a metabolic hub. [Abstract]2024 May:71:103112. PMID: 38461791 -
Research (Wash D C)
Hyperbaric Oxygen Boosts Antitumor Efficacy of Copper-Diethyldithiocarbamate Nanoparticles against Pancreatic Ductal Adenocarcinoma by Regulating Cancer Stem Cell Metabolism. [Abstract]2024 Mar 11:7:0335. PMID: 38766644 -
Pharmacol Res
Glutamine deprivation confers immunotherapy resistance by inhibiting IFN-γ signaling in cancer cells. [Abstract]2025 Feb 3:213:107643. PMID: 39909124 -
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Int J Biol Macromol
Integrated multi-omics and functional analysis uncovers the structural and regulatory significance of Nucleophosmin 1 in the metabolic progression of esophageal carcinoma. [Abstract]2025 Dec 9:149610. PMID: 41380889 -
Int J Biol Macromol
Lycium barbarum polysaccharide mitigates high-fat-diet-induced skeletal muscle atrophy by promoting AMPK/PINK1/Parkin-mediated mitophagy. [Abstract]2025 Jan 29:301:140488. PMID: 39889999 -
Phytomedicine
Phlorizin from Lithocarpus litseifolius [Hance] Chun ameliorates FFA-induced insulin resistance by regulating AMPK/PI3K/AKT signaling pathway. [Abstract]2024 Jul 25:130:155743. PMID: 38824822 -
Phytomedicine
Bergenin alleviates proliferative arterial diseases by modulating glucose metabolism in vascular smooth muscle cells. [Abstract]2024 Jul:129:155592. PMID: 38608597 -
ACS Appl Mater Interfaces
NIR-Activated Hollow Upconversion Nanocomposites for Tumor Therapy via GLUT1 Inhibition and Mitochondrial Function Disruption. [Abstract]2025 Apr 1. PMID: 40166910 -
Br J Pharmacol
HSP90β shapes the fate of Th17 cells with the help of glycolysis-controlled methylation modification. [Abstract]2024 Oct;181(20):3886-3907. PMID: 38881036 -
J Transl Med
DARS2 overexpression is associated with PET/CT metabolic parameters and affects glycolytic activity in lung adenocarcinoma. [Abstract]2023 Aug 26;21(1):574. PMID: 37626419 -
Oncogene
Cancer-associated fibroblasts promote EGFR-TKI resistance via the CTHRC1/glycolysis/H3K18la positive feedback loop. [Abstract]2025 May;44(19):1400-1414. PMID: 40011576 -
Microbiol Res
2025 Sep 24:302:128350. PMID: 41014824 -
Neural Regen Res
Perfluoropentane-based oxygen-loaded nanodroplets reduce microglial activation through metabolic reprogramming. [Abstract]2025 Apr 1;20(4):1178-1191. PMID: 38989955 -
Antioxidants (Basel)
Hyperosmolarity-Induced Oxidative Stress Leads to Senescence in Human Corneal Epithelial Cells (HCEPC) via DNA Damage, Metabolic Disturbance and Mitophagy Decline. [Abstract]2025 Nov 19;14(11):1381. PMID: 41300537 -
Oncogenesis
Methylation of HBP1 by PRMT1 promotes tumor progression by regulating actin cytoskeleton remodeling. [Abstract]2022 Aug 8;11(1):45. PMID: 35941115 -
Biomater Adv
Matrix stiffness modulates YAP-mediated glycolysis and proliferation in human corneal endothelial cells. [Abstract]2026 Mar:180:214594. PMID: 41218465 -
Mar Drugs
Novel PPAR-γ Agonist from the Soft Coral Sarcophyton crassocaule: Modulating Glucose Uptake and Lipid Droplet Formation. [Abstract]2025 Nov 24;23(12):450. PMID: 41440884 -
Chem Biol Interact
Palmitic acid-induced insulin resistance triggers granulosa cell senescence by disruption of the UPRmt/mitophagy/lysosome axis. [Abstract]2025 Apr 25:411:111450. PMID: 40023272 -
Food Funct
Puerarin improves hepatic glucose and lipid homeostasis in vitro and in vivo by regulating the AMPK pathway. [Abstract]2021 Mar 21;12(6):2726-2740. PMID: 33681875
2-NBDG purchased from MedChemExpress. Usage Cited in: Food Funct. 2021 Mar 21;12(6):2726-2740. [Abstract]
Glucose absorption and photomicrographs of HepG2 cells stained with 2-NBDG (100 μM; for 30 min).
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Mar Drugs
Fucoidan from Ascophyllum nodosum Suppresses Postprandial Hyperglycemia by Inhibiting Na+/Glucose Cotransporter 1 Activity. [Abstract]2020 Sep 22;18(9):485. PMID: 32971911
2-NBDG purchased from MedChemExpress. Usage Cited in: Mar Drugs. 2020 Sep 22;18(9):485. [Abstract]
Caco-2 cells are treated with 100 μM 2‐NBDG for 30 min. Only 400 μg/mL of an AnF solution could significantly inhibit the transport of 2-NBDG in this Caco-2 monolayer cell model compared with the Control group, while the same concentration of LjF and KcF could not.
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Life Sci
p38MAPK protects against myocardial ischemia-reperfusion injury by regulating GLUT4 expression and translocation through MEF2A/MEF2C in isolated rat heart. [Abstract]2026 May 15:393:124343. PMID: 41865872 -
Life Sci
Simvastatin ameliorates adverse pregnancy by inhibiting glycolysis-related NETs in obstetrical antiphospholipid syndrome. [Abstract]2024 Nov 4:123215. PMID: 39505298 -
Inflammation
2023 Aug;46(4):1445-1457. PMID: 37171694 -
Expert Opin Drug Discov
In-silico guided identification and in-vitro studies of potential FFAR4 agonists for type 2 diabetes mellitus therapy. [Abstract]2025 Aug;20(8):1075-1092. PMID: 40580106 -
Int J Mol Sci
Increased Oxidative Phosphorylation Is Required for Stemness Maintenance in Liver Cancer Stem Cells from Hepatocellular Carcinoma Cell Line HCCLM3 Cells. [Abstract]2020 Jul 25;21(15):5276. PMID: 32722385 -
World J Diabetes
Genipin relieves diabetic retinopathy by down-regulation of advanced glycation end products via the mitochondrial metabolism related signaling pathway. [Abstract]2023 Sep 15;14(9):1349-1368. PMID: 37771331 -
Lung Cancer
SFXN1 as a potential diagnostic and prognostic biomarker of LUAD is associated with 18F-FDG metabolic parameters. [Abstract]2024 Feb:188:107449. PMID: 38184958 -
Biochim Biophys Acta Mol Basis Dis
Triiodothyronine promotes the proliferation and chemoresistance of cholangiocarcinoma cells via HIF-1α/Glut1-stimulated glycolysis. [Abstract]2025 Mar 31;1871(5):167814. PMID: 40168755 -
Mol Nutr Food Res
Apigenin Ameliorates Insulin Resistance in 3T3-L1 Adipocytes: Establishment of a New Insulin Resistance Model Induced by Combined Treatments. [Abstract]2025 Mar;69(5):e202400545. PMID: 39945085 -
J Cell Mol Med
Dihydromyricetin resists inflammation-induced muscle atrophy via ryanodine receptor-CaMKK-AMPK signal pathway. [Abstract]2021 Nov;25(21):9953-9971. PMID: 34676967 -
FASEB J
Angptl7 promotes insulin resistance and type 2 diabetes mellitus by multiple mechanisms including SOCS3-mediated IRS1 degradation. [Abstract]2020 Oct;34(10):13548-13560. PMID: 32786125 -
J Cell Mol Med
2022 Jul;26(13):3783-3796. PMID: 35656815 -
iScience
Hypoxia promotes histone H3K9 lactylation to enhance LAMC2 transcription in esophageal squamous cell carcinoma. [Abstract]2024 Jun 5;27(7):110188. PMID: 38989468 -
Virol Sin
Thapsigargin suppresses porcine hemagglutinating encephalomyelitis virus replication by disrupting host energy metabolism. [Abstract]2025 Nov 21:S1995-820X(25)00161-0. PMID: 41276220 -
J Cell Physiol
BKCa channel participates in insulin-induced lipid deposition in adipocytes by increasing intracellular calcium. [Abstract]2021 Aug;236(8):5818-5831. PMID: 33432604
2-NBDG purchased from MedChemExpress. Usage Cited in: J Cell Physiol. 2021 Aug;236(8):5818-5831. [Abstract]
The cells are treated with 10 μM 2‐NBDG in serum‐free DMEM/F12 for 30 min.
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Ann Clin Transl Neurol
2026 Mar 24. PMID: 41876403 -
Sci Rep
Dysregulated GLUT1 results in the pathogenesis of preeclampsia by impairing the function of trophoblast cells. [Abstract]2024 Oct 10;14(1):23761. PMID: 39390043 -
Biomedicines
Development of a Competitive Nutrient-Based T-Cell Immunotherapy Designed to Block the Adaptive Warburg Effect in Acute Myeloid Leukemia. [Abstract]2024 Oct 3;12(10):2250. PMID: 39457563 -
Cell Signal
KNDC1 attenuates fibrosis in nonalcoholic fatty liver disease by inhibiting glycolysis in hepatic stellate cells. [Abstract]2026 Jul:143:112487. PMID: 41833773 -
Eur J Immunol
High Extracellular K+ Skews T-Cell Differentiation Towards Tumour Promoting Th2 and Treg Subsets. [Abstract]2025 Feb;55(2):e202451440. PMID: 39651799 -
Cell Signal
DDX24 promotes tumor progression by mediating hexokinase-1 induced glycolysis in gastric cancer. [Abstract]2024 Feb:114:110995. PMID: 38043669 -
Heliyon
gys1 regulates maternal glycogen reserve essential for embryonic development in zebrafish. [Abstract]2024 May 14;10(10):e31149. PMID: 38803914 -
Biol Trace Elem Res
Antagonizing Effects of Chromium Against Iron-Decreased Glucose Uptake by Regulating ROS-Mediated PI3K/Akt/GLUT4 Signaling Pathway in C2C12. [Abstract]2024 Feb;202(2):701-712. PMID: 37156991 -
J Sci Food Agric
Spheroidization of ultrasonic degraded corn silk polysaccharide to enhance bioactivity by the anti-solvent precipitation method. [Abstract]2022 Jan 15;102(1):53-61. PMID: 34031881 -
Arch Med Res
Monocarboxylate Transporter 4 Inhibition Reduces Synovial Hyperproliferation and Metabolic Reprogramming Under Hypoxia in Rheumatoid Arthritis. [Abstract]2025 Aug 18;57(1):103283. PMID: 40829205 -
Toxicol Appl Pharmacol
Downregulation of HSPA12A underlies myotoxicity of local anesthetic agent bupivacaine through inhibiting PGC1α-mediated mitochondrial integrity. [Abstract]2022 Jan 1:434:115798. PMID: 34793778 -
Front Oncol
CD147 Mediates 5-Fluorouracil Resistance in Colorectal Cancer by Reprogramming Glycolipid Metabolism. [Abstract]2022 Jul 11:12:813852. PMID: 35898887 -
Diabetes Metab Syndr Obes
2025 Jul 29:18:2573-2584. PMID: 40755745 -
J Diabetes Investig
Adipocyte exosome miR-4472 inhibits glucose uptake in skeletal muscle through downregulation of MEF2D. [Abstract]2025 Aug;16(8):1382-1397. PMID: 40342203 -
Mol Immunol
D-mannose acts as a V-ATPase inhibitor to suppress inflammatory cytokines generation and bacterial killing in macrophage. [Abstract]2023 Oct:162:84-94. PMID: 37660434 -
Vet Microbiol
HDAC4 suppresses porcine epidemic diarrhea virus infection through negatively regulating MEF2A-GLUT1/3 axis- mediated glucose uptake. [Abstract]2025 Apr 11:305:110520. PMID: 40250106 -
Animals (Basel)
RNA-Seq Analysis of Glycolysis Regulation of Avian Leukosis Virus Subgroup J Replication. [Abstract]2024 Aug 28;14(17):2500. PMID: 39272286 -
Brain Res
A peptide drug targeting SASH1-PKM2 interaction promotes recovery of traumatic brain injury in mice. [Abstract]2026 Apr 15:1877-1878:150206. PMID: 41690666 -
Biochem Biophys Res Commun
miR-92a-1-5p targets MEF2A to induce insulin resistance in myocardial ischemia/reperfusion injury. [Abstract]2025 Jul 1:768:151938. PMID: 40345013 -
Biochem Biophys Res Commun
GPR30 selective agonist G-1 induced insulin resistance in ovariectomized mice on high fat diet and its mechanism. [Abstract]2024 Jul 5:716:150026. PMID: 38701557 -
Biochem Biophys Res Commun
LIGHT (TNFSF14) inhibits glucose uptake of adipocytes by downregulating GLUT4 expression via AKT signaling pathway. [Abstract]2021 Oct 28:583:106-113. PMID: 34735871 -
Genes Genomics
Targeting phosphofructokinase 2 the isoform PFKFB4 suppresses glioblastoma proliferation and malignancy. [Abstract]2025 Oct 21. PMID: 41118116 -
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Oxid Med Cell Longev
Attenuation of ROS/Chloride Efflux-Mediated NLRP3 Inflammasome Activation Contributes to Alleviation of Diabetic Cardiomyopathy in Rats after Sleeve Gastrectomy. [Abstract]2022 Apr 19;2022:4608914. PMID: 35498125
2-NBDG purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2022 Apr 19;2022:4608914. [Abstract]
H9c2 cells are incubated with 2-NBDG (50 μM; for 30 min). Flow cytometry quantified the 2-NBDG signals in HG-stimulated H9c2 cells.
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Oxid Med Cell Longev
NOX4-Derived ROS Mediates TGF- β 1-Induced Metabolic Reprogramming during Epithelial-Mesenchymal Transition through the PI3K/AKT/HIF-1 α Pathway in Glioblastoma. [Abstract]2021 Jun 27;2021:5549047. PMID: 34257808
2-NBDG purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2021 Jun 27;2021:5549047. [Abstract]
Immunofluorescence images results of glucose uptake in glioblastoma cells determined by staining with 2-NBDG (50 μM; for 30 min) after treated with TGF-β1 in the absence or presence of GKT137831.
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Oxid Med Cell Longev
Dihydromyricetin Ameliorates Inflammation-Induced Insulin Resistance via Phospholipase C-CaMKK-AMPK Signal Pathway. [Abstract]2021 Oct 5;2021:8542809. PMID: 34650665
2-NBDG purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2021 Oct 5;2021:8542809. [Abstract]
3T3-L1 are incubated with or without media containing 10 nM insulin for 10 minutes. The media are changed to low-glucose DMEM containing 150 μg/mL 2-NBDG for 60 minutes at 37°C.
Solvent & Solubility
DMSO : 33.33 mg/mL (97.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 5 mg/mL (14.61 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Purity & Documentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Yamada K, et al. A real-time method of imaging glucose uptake in single, living mammalian cells. Nat Protoc. 2007;2(3):753-62. [Content Brief]
[2]. Katsuya Yamada, et al. A real-time method of imaging glucose uptake in single, living mammalian cells. Nat Protoc. 2007;2(3):753-62. [Content Brief]
[3]. Zou C, et al. 2-NBDG as a fluorescent indicator for direct glucose uptake measurement. J Biochem Biophys Methods. 2005 Sep 30;64(3):207-15. [Content Brief]
[4]. Zou C, et al. 2-NBDG as a fluorescent indicator for direct glucose uptake measurement. J Biochem Biophys Methods. 2005 Sep 30;64(3):207-15. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 2.9218 mL | 14.6088 mL | 29.2176 mL | 73.0439 mL |
| 5 mM | 0.5844 mL | 2.9218 mL | 5.8435 mL | 14.6088 mL | |
| 10 mM | 0.2922 mL | 1.4609 mL | 2.9218 mL | 7.3044 mL | |
| DMSO | 15 mM | 0.1948 mL | 0.9739 mL | 1.9478 mL | 4.8696 mL |
| 20 mM | 0.1461 mL | 0.7304 mL | 1.4609 mL | 3.6522 mL | |
| 25 mM | 0.1169 mL | 0.5844 mL | 1.1687 mL | 2.9218 mL | |
| 30 mM | 0.0974 mL | 0.4870 mL | 0.9739 mL | 2.4348 mL | |
| 40 mM | 0.0730 mL | 0.3652 mL | 0.7304 mL | 1.8261 mL | |
| 50 mM | 0.0584 mL | 0.2922 mL | 0.5844 mL | 1.4609 mL | |
| 60 mM | 0.0487 mL | 0.2435 mL | 0.4870 mL | 1.2174 mL | |
| 80 mM | 0.0365 mL | 0.1826 mL | 0.3652 mL | 0.9130 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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