Cat. No.: HY-N0018

Daidzin

(Synonyms: Daidzoside; NPI-031D; Daidzein 7-O-glucoside)

13 Cited Publications

Customer Review

Based on 13 publication(s) in Google Scholar

Daidzin is an isoflavone with antioxidant, anticancer, and antiatherosclerotic activities. Daidzin is a potent and selective inhibitor of mitochondrial ALDH-2. Daidzin reduces ethanol consumption.

For research use only. We do not sell to patients.

Purity: 99.65%
CAS No.: 552-66-9
Formula: C₂₁H₂₀O₉
Molecular Weight:416.38

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Daidzin Chemical Structure

CAS No.: 552-66-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (13)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Aldehyde Dehydrogenase (ALDH) Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HepG2	IC₅₀	61.2 μM Compound: 1	Anticancer activity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay Anticancer activity against human HepG2 cells assessed as cell viability after 24 hrs by MTT assay	[PMID: 24974349]

In Vitro

Daidzin inhibits hamster and rat ALDH-2, with K_is of 0.082 and 0.052 μM respectively^[1].
Daidzin (500 μM) shows antioxidant activity, measured by its ability to inhibit cytochrome c reduction by Phorbol myristic acetate (PMA)-stimulated neutrophils^[3].
Daidzin (0-30 μM, 30 min) inhibits the formation of 5-HIAA (IC₅₀ value of ≈2.7 μM), and increases the amounts of 5-HIAL and 5-HTOL in the incubation media of hamster liver mitochondrial^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Daidzin (30 mg/kg, intragastrically) decreases blood alcohol levels (induced by 3 g ethanol/kg body weight), and shortens sleep time induced by Ethanol intoxication (7 g/kg by intubation) in fasted rats^[3].
Daidzin (70 meq/hamster/day, i.p.) inhibits hamster ethanol intake by 62%^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 552-66-9
Appearance Solid
Molecular Weight 416.38
Formula C₂₁H₂₀O₉
Color White to off-white
SMILES

OC1=CC=C(C2=COC(C=C(O[C@H]3[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O3)C=C4)=C4C2=O)C=C1
Synonyms

Daidzoside; NPI-031D; Daidzein 7-O-glucoside
Structure Classification
- Flavonoids
- Isoflavones
- Phenols
- Monophenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (13)

All Cell Imaging/StainingBio/Physico-chemical AssayWBFlow CytometryIn Vivo Imaging

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Journal Impact Factor

Cell Mol Immunol

Neutrophil ALDH2 is a new therapeutic target for the effective treatment of sepsis-induced ARDS. [Abstract]

2024 May;21(5):510-526. PMID: 38472357

Daidzin purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2024 May;21(5):510-526. [Abstract]

Representative immunofluorescence images showing NET formation in human peripheral blood neutrophils (Daidzin (20 μM)).

Daidzin purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2024 May;21(5):510-526. [Abstract]

The accumulation of H3Cit was significantly decreased by Alda-1 treatment and increased by Daidzin (20 μM) treatment, as observed in both ALDH2^wt and ALDH2^rs671 mutant neutrophils.
Adv Sci (Weinh)

Targeting Caveolin-1 in Multiple Myeloma Cells Enhances Chemotherapy and Natural Killer Cell-Mediated Immunotherapy. [Abstract]

2024 Dec 4:e2408373. PMID: 39630017

Daidzin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Dec 4:e2408373. [Abstract]

MM1S cells were treated with 6‐MP or Daidzin (0-20 μM) at the indicated concentrations for 48 h and the expression of CAV1 was detected using western blot.

Daidzin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Dec 4:e2408373. [Abstract]

MM1S cells were treated with Bortezomib (1.25 nM) in combination with 6‐MP or Daidzin (20 μM) for 48 h and cell apoptosis was determined using flow cytometry.

Daidzin purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Dec 4:e2408373. [Abstract]

B-NDG mice were inoculated with MM1S-Luc cells and randomly divided into six groups (n = 5-7). After 11 days of inoculation, these mice were intraperitoneally injected with 6-MP (10-20 mg/kg), Daidzin (30 mg/kg), or vehicle daily until day 67. After 15 days of inoculation, mice were intraperitoneally injected with bortezomib (0.5 mg/kg) three times a week until day 67. The distribution of MM1S-Luc cells in these mice was measured at the indicated days after inoculation using a living imaging system.
Food Chem

Distribution profiles of 12 isoflavone monomers in Chinese soybeans from saline-alkali and ordinary soils and subsequent supercritical CO₂ extraction optimization. [Abstract]

2026 Jul 30:518:149561. PMID: 42114489
Food Chem

Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]

2025 Dec 30:497:146992. PMID: 41285060
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 May 31:489:144992. PMID: 40466530
Free Radical Bio Med

Disulfiram suppresses NLRP3 inflammasome activation to treat peritoneal and gouty inflammation. [Abstract]

2020 May 20;152:8-17. PMID: 32151746
J Ethnopharmacol

Discovery of bakuchiol as an AIM2 inflammasome activator and cause of hepatotoxicity. [Abstract]

2022 Nov 15:298:115593. PMID: 35973629
Eur J Pharmacol

Acetaldehyde dehydrogenase 2 attenuates lipopolysaccharide -induced endothelial barrier damage by inhibiting mitochondrial fission in sepsis-associated encephalopathy. [Abstract]

2025 Jun 15:997:177468. PMID: 40054720
Biochim Biophys Acta Mol Basis Dis

Loss of ALDH2 accelerates the progression of pulmonary arterial hypertension through the 4-HNE/ERK1/2-p16INK4a signaling pathway. [Abstract]

2025 Apr 22:167863. PMID: 40274079
Mol Med Rep

Pretreatment with the ALDH2 activator Alda‑1 protects rat livers from ischemia/reperfusion injury by inducing autophagy. [Abstract]

2020 Sep;22(3):2373-2385. PMID: 32705206
Neurochem Res

ALDH2 Attenuates Blood-Brain Barrier Injury Induced by Cerebral Ischemia/Reperfusion via Alleviating ROS/NLRP3 Inflammasome Axis. [Abstract]

2025 Jul 3;50(4):224. PMID: 40608173
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
Biochem Biophys Res Commun

Estrogen ameliorates sepsis-induced vascular hyporeactivity in thoracic aorta of female rats via permissive effect of GRα expression. [Abstract]

2023 May 21:657:108-118. PMID: 37002984

Solvent & Solubility

In Vitro:

DMSO : ≥ 200 mg/mL (480.33 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4017 mL

12.0083 mL

24.0165 mL

5 mM

0.4803 mL

2.4017 mL

4.8033 mL

10 mM

0.2402 mL

1.2008 mL

2.4017 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (5.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.08 mg/mL (5.00 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (5.00 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 15% Cremophor EL 85% Saline

Solubility: 25 mg/mL (60.04 mM); Suspended solution; Need ultrasonic

Protocol 2

Add each solvent one by one: 0.5% CMC-Na/1% Tween-80 in Saline water

Solubility: 39.6 mg/mL (95.11 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (276 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Xie CI, et al. Daidzin, an antioxidant isoflavonoid, decreases blood alcohol levels and shortens sleep time induced by ethanol intoxication. Alcohol Clin Exp Res. 1994 Dec;18(6):1443-7. [Content Brief]

[2]. Keung WM, et al. Kudzu root: an ancient Chinese source of modern antidipsotropic agents. Phytochemistry. 1998 Feb;47(4):499-506. [Content Brief]

[3]. Keung WM, et al. Daidzin inhibits mitochondrial aldehyde dehydrogenase and suppresses ethanol intake of Syrian golden hamsters. Proc Natl Acad Sci U S A. 1997 Mar 4;94(5):1675-9. [Content Brief]

[4]. Keung WM, Vallee BL. Daidzin and its antidipsotropic analogs inhibit serotonin and dopamine metabolism in isolated mitochondria. Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2198-203. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.4017 mL	12.0083 mL	24.0165 mL	60.0413 mL
	5 mM	0.4803 mL	2.4017 mL	4.8033 mL	12.0083 mL
	10 mM	0.2402 mL	1.2008 mL	2.4017 mL	6.0041 mL
	15 mM	0.1601 mL	0.8006 mL	1.6011 mL	4.0028 mL
	20 mM	0.1201 mL	0.6004 mL	1.2008 mL	3.0021 mL
	25 mM	0.0961 mL	0.4803 mL	0.9607 mL	2.4017 mL
	30 mM	0.0801 mL	0.4003 mL	0.8006 mL	2.0014 mL
	40 mM	0.0600 mL	0.3002 mL	0.6004 mL	1.5010 mL
	50 mM	0.0480 mL	0.2402 mL	0.4803 mL	1.2008 mL
	60 mM	0.0400 mL	0.2001 mL	0.4003 mL	1.0007 mL
	80 mM	0.0300 mL	0.1501 mL	0.3002 mL	0.7505 mL
	100 mM	0.0240 mL	0.1201 mL	0.2402 mL	0.6004 mL

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Daidzin

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Publications Citing Use of MedChemExpress (MCE) Daidzin

All Aldehyde Dehydrogenase (ALDH) Isoforms

Biological Activity

Chemical Information

Publications (13)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Daidzin Related Classifications

HY-N0018 Related Classifications