WST-1
Based on 5 publication(s) in Google Scholar
WST-1 is a kind of water-soluble tetrazolium salt. WST induces the intracellular mitochondrial dehydrogenase to conduct NADH-dependent enzyme digestion reaction, releasing the water-soluble methyl benzene product. WST-1 can be used for the detection of cell proliferation and cytotoxicity, via the determination of the light absorption value at 450 nm.
For research use only. We do not sell to patients.
- Purity: 97.30%
- CAS No.: 150849-52-8
- Formula: C19H11IN5NaO8S2
- Molecular Weight:651.34
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) WST-1
More
Customer Validation & Images
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Bio/Physico-chemical Assay
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Cell Proliferation/Viability Assay
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Cell Proliferation/Viability Assay
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Preparation of WST-1 working solution
1.1 Preparation of the stock solution
Dissolve 10 mg of WST-1 in 1 mL of DMSO to obtain 10 mg/mL of WST-1.
1.2 Preparation of WST-1 working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 5-10 μM of WST-1 working solution.
Note: Please adjust the concentration of WST-1 working solution according to the actual situation.
Cell staining
2.1 Cell preparation.
For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of WST-1 working solution and 1-methoxy PMS (2 mM), and then incubate at room temperature for 2 h.
2.3 Use microplate reader detect 450/690 nm OD.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 150849-52-8
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Appearance Solid
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Molecular Weight 651.34
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Formula C19H11IN5NaO8S2
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Color Light yellow to brown
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SMILES
O=[S]([O-])(C1=C(C=CC([S](=O)(O[Na])=O)=C1)C2=NN(C(C=C3)=CC=C3I)[N+](C(C=C4)=CC=C4[N+]([O-])=O)=N2)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)
Publications (5)
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Journal Impact Factor
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Most Recent
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Biomaterials
2022 Feb;281:121341. PMID: 34995901 -
Cell Rep
mTORC1 regulates the pyrimidine salvage pathway by controlling UCK2 turnover via the CTLH-WDR26 E3 ligase. [Abstract]2025 Jan 13;44(1):115179. PMID: 39808525
WST-1 purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 Jan 13;44(1):115179. [Abstract]
Cell survival was measured using WST-1 (1 mg/mL; 3 h) in HCT116 UCK2-FKBP12F36V K/I cells treated with the indicated concentrations of 5-azacytidine or 5-fluorouridine with or without dTAGv1.
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J Funct Biomater
ATP-Responsive Bimetallic Metal-Organic Frameworks Amplify Oxidative Stress in the Tumor Microenvironment for Synergistic Chemo-Immunotherapy. [Abstract]2026 Apr 19;17(4):199. PMID: 42042305 -
J Pharm Biomed Anal
Development of rapid diagnostic kit against sepsis: Barcode-based semi-quantitative measurement of C-reactive protein and lactate. [Abstract]2026 Oct 15:279:117580. PMID: 42190402
WST-1 purchased from MedChemExpress. Usage Cited in: J Pharm Biomed Anal. 2026 Oct 15:279:117580. [Abstract]
Calibration curves for lactate analysis. Standards or prediluted samples were mixed with reaction buffer containing 5 mM WST-1 and 0.2 mM methoxy-PMS in 0.1 M Tris buffer (pH 8.0) at a 1:1 ratio. A 7 μL aliquot of the mixture was added to each well and incubated for 15 min at room temperature, after which the absorbance was measured at 440 nm.
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Sci Total Environ
Assessment of immunomodulatory effects of five commonly used parabens on human THP-1 derived macrophages: Implications for ecological and human health impacts. [Abstract]2024 Jun 6:173823. PMID: 38851341
WST-1 purchased from MedChemExpress. Usage Cited in: Sci Total Environ. 2024 Jun 6:173823. [Abstract]
Cell viability was measured using WST-1 (1 mg/mL; 1 h) in human THP-1-derived macrophages exposed to paraben under basal or LPS-challenge conditions.
Solvent & Solubility
H2O : 87.5 mg/mL (134.34 mM; Need ultrasonic)
DMSO : 25 mg/mL (38.38 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 1.5353 mL | 7.6765 mL | 15.3530 mL | 38.3824 mL |
| 5 mM | 0.3071 mL | 1.5353 mL | 3.0706 mL | 7.6765 mL | |
| 10 mM | 0.1535 mL | 0.7676 mL | 1.5353 mL | 3.8382 mL | |
| 15 mM | 0.1024 mL | 0.5118 mL | 1.0235 mL | 2.5588 mL | |
| 20 mM | 0.0768 mL | 0.3838 mL | 0.7676 mL | 1.9191 mL | |
| 25 mM | 0.0614 mL | 0.3071 mL | 0.6141 mL | 1.5353 mL | |
| 30 mM | 0.0512 mL | 0.2559 mL | 0.5118 mL | 1.2794 mL | |
| H2O | 40 mM | 0.0384 mL | 0.1919 mL | 0.3838 mL | 0.9596 mL |
| 50 mM | 0.0307 mL | 0.1535 mL | 0.3071 mL | 0.7676 mL | |
| 60 mM | 0.0256 mL | 0.1279 mL | 0.2559 mL | 0.6397 mL | |
| 80 mM | 0.0192 mL | 0.0960 mL | 0.1919 mL | 0.4798 mL | |
| 100 mM | 0.0154 mL | 0.0768 mL | 0.1535 mL | 0.3838 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
WST-1 Related Classifications
HY-136976 Related Classifications
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