IKKε-IN-1
IKKε-IN-1 is a IKKε inhibitor. IKKε-IN-1 reduces cell viability, inhibits colony formation and cell migration. IKKε-IN-1 induces autophagy (Autophagy) in cancer cells. IKKε-IN-1 can be used in the research of cancers including colorectal cancer, hepatocellular carcinoma, bladder cancer, breast cancer, lung cancer, and cervical cancer.
For research use only. We do not sell to patients.
- CAS No.: 3035441-37-0
- Formula: C26H28N2O3
- Molecular Weight:416.51
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
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IKKε |
In Vitro
IKKε-IN-1 (6.25-50 μM; 72 h) reduces the viability of HCT116, HepG2, T24, MDA-MB-231, A549 and Hela cells, with the strongest activity against HCT116 colorectal cancer cells (IC50 = 13.37 μM)[1].
IKKε-IN-1 (6.25-50 μM; 2-14 days) inhibits the long-term proliferation of HCT116 and HepG2 cells, and reduces the migration of HCT116 cells[1].
IKKε-IN-1 (12.5-50 μM; 48 h) induces autophagosome formation but blocks autophagic flux in HCT116 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HCT116、HepG2、T24、MDA-MB-231、A549、Hela cells
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Concentration:6.25、12.5、25、50 μM
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Incubation Time:72 h
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Result:Reduced cell viability in all six cancer cell lines in a concentration-dependent manner.
Exhibited IC50 values of 13.37 μM (HCT116), 17.44 μM (HepG2), 29.733 μM (T24), 32.48 μM (MDA-MB-231), 66.3 μM (A549), and 68.05 μM (HeLa).
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Cell Line:HCT116, HepG2 cells
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Concentration:12.5、25、50 μM
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Incubation Time:14 days
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Result:Reduced colony formation in both cell lines in a concentration-dependent manner.
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Cell Line:HCT116 cells
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Concentration:6.25、12.5 μM
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Incubation Time:48 h
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Result:Inhibited the migration, had a more pronounced effect on reducing
cell migration at 12.5 μM.
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Cell Line:HCT116 cells
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Concentration:12.5、25、50 μM
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Incubation Time:48 h
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Result:Increased the LC3B-II/LC3B-I ratio in a concentration-dependent manner, indicating increased autophagosome formation, and also increased p62 levels in a concentration-dependent manner, indicating blocked autophagic flux.
Significantly increased the number of yellow autophagosome puncta, confirming impaired fusion of autophagosomes and lysosomes.
- Scratch/Wound-Healing Migration Assay
- Mammalian live/dead viability and cytotoxicity staining
- Cell migration
- Neural Crest/Neuronal Cell Migration Explant Assay
- CCK-8/WST-8 Cell Proliferation Assay
- Liver Cancer Modeling
- Colony Formation (Clonogenic) Assay
- Breast Cancer Modeling
- Cell Viability Determination by MTT Colorimetric Assay
- Cell Cytotoxicity Assay
- Zigmond Chamber Chemotaxis Assay
- Under-Agarose Cell Migration Assay
- Transwell/Boyden Chamber Migration Assay
- Autophagy
- 3D Collagen/Hydrogel Matrix Migration Assay
- Soft Agar Colony Formation Assay
- Lysosome and acidic-vesicle live-cell staining
- MTT Cell Proliferation Assay
- Cell-Exclusion Zone Migration Assay
- Dunn/Insall Chamber Chemotaxis Assay
- Macroautophagy Solutions
Chemical Information
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CAS No. 3035441-37-0
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Molecular Weight 416.51
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Formula C26H28N2O3
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SMILES
[H][C@]12CCCCN1CCC[C@H]2CNC(C3=CC=CC(C4=CC5=CC=CC=C5OC4=O)=C3)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
IKKε-IN-1 Related Classifications
HY-181271 Related Classifications
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