Isatin-O  (Synonyms: Isatin β-oxime; Isatin 3-oxime)

Isatin-O (Isatin β-oxime; Isatin 3-oxime) is an acetylcholinesterase (AChE) reactivator capable of crossing the blood-brain barrier. Isatin-O reactivates paraoxon-inhibited acetylcholinesterase in the central and peripheral nervous systems. As a glycosylation substrate, Isatin-O undergoes complete glycosylation in a Rauvolfia serpentina cell suspension culture system to form isatin-3-oxime-glucoside. Isatin-O can be used in studies related to organophosphate poisoning^[1][2].

Isatin-O (10-100 μmol/L; 10-120 min) reactivates paraoxon-inhibited Electrophorus electricus acetylcholinesterase, reaching ~8% reactivation at 10 μmol/L within 10 min and ~23% reactivation at 100 μmol/L after 120 min, with better initial reactivation performance than 2-PAM at 10 μmol/L^[1].
Isatin-O (5.76 mM; 72 h) is completely glucosylated by Rauvolfia serpentina plant cell suspension cultures to form Isatin-3-oxime-β-D-glucopyranoside within 72 h, with the glucoside accumulating intracellularly without further metabolism^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

162.15

C₈H₆N₂O₂

607-28-3

O=C1NC=2C=CC=CC2C1=NO

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. de Paula RL, et al. Molecular Modeling and In Vitro Studies of a Neutral Oxime as a Potential Reactivator for Acetylcholinesterase Inhibited by Paraoxon. Molecules (Basel, Switzerland). 2018 Nov 12;23(11):2954.

  [2]. Hinse C, et al. Glucosylation of isatin-3-oxime followed by 2D in situ NMR in plant cells at highest magnetic field without labelling. Natural product letters. 2001;15(2):119-24.