Nagilactone B

RAW264.7 cells are plated into 12-well plates at 3×10⁵ cells per well. After 12 h, cells are co-incubated with oxLDL (20 μg/mL) with or without Nagilactone B (0.02, 0.1, 0.5 μM) in DMEM for 24 h. At the end of the treatment period, cells are washed twice with PBS, fixed with 4% paraformaldehyde, and stained by filtered 0.5% ORO. Images are obtained via DP70 microscopy from at least six randomly selected fields for each condition. Data are analysed with Image-Pro Plus 6.0 software. The ratio of foam cells is calculated as the percentage of stained to total cell area^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Mice^[1]
ApoE^-/- mice at 6 weeks of age on a C57BL/6J background are randomly divided into five groups [normal diet, model, Atorvastatin, NLB (L), and NLB (H) groups]. Atorvastatin and NLB are dissolved in CMC-Na before administered. Mice on the normal diet receive carboxymethylcellulose sodium (CMC-Na) as the negative control group. Mice on a high-fat diet (HFD) (0.15% cholesterol and 18% fat) are administered CMC-Na or Atorvastatin (10 mg/kg) or Nagilactone B [10 and 30 mg/kg, as NLB (L) and NLB (H), respectively] daily in an intragastric manner for 12 weeks. Body weight and food intake are monitored^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.