1. Cell Cycle/DNA Damage
    Epigenetics
  2. HDAC
  3. J22352

J22352 

Cat. No.: HY-126147
Handling Instructions

J22352 is a PROTAC (proteolysis-targeting chimeras)-like and highly selective HDAC6 inhibitor with an IC50 value of 4.7 nM. J22352 promotes HDAC6 degradation and induces anticancer effects by inhibiting autophagy and eliciting the antitumor immune response in glioblastoma cancers, and leading to the restoration of host antitumor activity by reducing the immunosuppressive activity of PD-L1.

For research use only. We do not sell to patients.

J22352 Chemical Structure

J22352 Chemical Structure

CAS No. : 2252395-44-9

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Description

J22352 is a PROTAC (proteolysis-targeting chimeras)-like and highly selective HDAC6 inhibitor with an IC50 value of 4.7 nM. J22352 promotes HDAC6 degradation and induces anticancer effects by inhibiting autophagy and eliciting the antitumor immune response in glioblastoma cancers, and leading to the restoration of host antitumor activity by reducing the immunosuppressive activity of PD-L1[1].

IC50 & Target[1]

HDAC6

4.7 nM (IC50)

In Vitro

J22352 (0.1-20 μM; 72 hours) decreases U87MG cell viability in a dose-dependent manner[1].
J22352 (10 μM; 24 hours) shows a dose-dependent decrease in HDAC6 protein abundance[1].

Cell Viability Assay[1]

Cell Line: U87MG cells
Concentration: 0.1 μM; 0.5 μM; 1μM; 2.5 μM; 5 μM; 10 μM; 20 μM
Incubation Time: 72 hours
Result: A dose-dependent decrease on U87MG cell proliferation.

Western Blot Analysis[1]

Cell Line: U87MG cells
Concentration: 10 μM
Incubation Time: 24 hours
Result: A dose-dependent decrease in aberrant overexpression of HDAC6 in glioblastoma.
In Vivo

J22352 (10 mg/kg; given i.p. per day for 14 days in male nude mice) results in a >80% tumor growth inhibition (TGI) rate. J22352 is well tolerated in mice[1].

Animal Model: Male nude mice (BALB/cAnN.Cg-Foxnlnu/CrlNarl, 4-6 weeks old)[1]
Dosage: 10 mg/kg
Administration: Given i.p.; per day for 14 days
Result: Marked anti-tumor effects and well tolerated in mice.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
Molecular Weight

415.44

Formula

C₂₄H₂₁N₃O₄

CAS No.

2252395-44-9

SMILES

O=C(C1=CC=CC=C1N2CC3=CC=C(C(NO)=O)C=C3)N(CCC4=CC=CC=C4)C2=O

Shipping

Room temperature in continental US; may vary elsewhere

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J22352
Cat. No.:
HY-126147
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J22352

Cat. No.: HY-126147