5(6)-CFDA
Based on 6 publication(s) in Google Scholar
5(6)-CFDA is a common aliphatic luciferin-line organism. CFDA conducts free diffusion into cells, and then it is hydrolyzed into carboxyl fluorescein (CF) by intracellular non-specific lipase. CF containing portion contains an additional negative charge so that it is better retained in cells, compared to fluorescein dyes.
For research use only. We do not sell to patients.
- Purity: 98.75%
- CAS No.: 124387-19-5
- Formula: C25H16O9
- Molecular Weight:460.39
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 5(6)-CFDA
More
Biological Activity
Guidelines (The following is our recommended protocol. This protocol only provides guidance and should be modified according to your specific needs).
Preparation of 5(6)-CFDA working solution
1. Preparation of stock solution
Dissolve 1 mg 5(6)-CFDA in 0.217 mL DMSO to obtain a 10 mM 5(6)-CFDA stock solution.
Note: It is recommended to store the stock solution at -20°C or -80°C protected from light and avoid repeated freeze-thaw cycles.
2. 5(6)-CFDA working solution
Dilute the stock solution with serum-free cell culture medium or PBS to obtain a 1-10 μM 5(6)-CFDA working solution.
Note: Please adjust the concentration of the 5(6)-CFDA working solution according to actual conditions.
Cell staining
1. Cell preparation:
Suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes, then discard the supernatant. Wash twice with PBS for 5 minutes each time.
Adherent cells: Remove the cell culture medium, add trypsin to dissociate the cells, and prepare a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes, then discard the supernatant. Wash twice with PBS for 5 minutes each time.
2. Add 1 mL 5(6)-CFDA working solution and incubate at room temperature for 30 minutes.
3. Centrifuge at 400 g at 4°C for 3-4 minutes and discard the supernatant.
4. Wash twice with PBS for 5 minutes each time.
5. Resuspend the cells in serum-free cell culture medium or PBS, then analyze using a fluorescence microscope or flow cytometer.
Notes
1. It is recommended to store the stock solution protected from light at -20°C or -80°C and avoid repeated freeze-thaw cycles.
2. Please adjust the concentration of the 5(6)-CFDA working solution according to actual conditions.
3. This product is for research use only and is not intended for use in drugs, household, or other purposes.
4. For your safety and health, please wear a lab coat and disposable gloves during operation.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 124387-19-5
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Appearance Solid
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Molecular Weight 460.39
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Formula C25H16O9
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Color White to yellow
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SMILES
CC(OC1=CC=C2C(OC3=C(C=CC(OC(C)=O)=C3)C24C5=CC(C(O)=O)=CC=C5C(O4)=O)=C1)=O.CC(OC6=CC=C7C(OC8=C(C=CC(OC(C)=O)=C8)C79C%10=CC=C(C(O)=O)C=C%10C(O9)=O)=C6)=O
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Synonyms
5-(6)-Carboxyfluorescein diacetate; CFDA
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (6)
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Journal Impact Factor
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Most Recent
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Theranostics
METTL14 aggravates endothelial inflammation and atherosclerosis by increasing FOXO1 N6-methyladeosine modifications. [Abstract]2020 Jul 11;10(20):8939-8956. PMID: 32802173 -
Phytomedicine
Vielanin K enhances doxorubicin-induced apoptosis via activation of IRE1α- TRAF2 - JNK pathway and increases mitochondrial Ca2 + influx in MCF-7 and MCF-7/MDR cells. [Abstract]2020 Nov;78:153329. PMID: 32896708 -
Microbiologyopen
The inhibitory effect of paeoniflorin on reactive oxygen species alleviates the activation of NF-κB and MAPK signalling pathways in macrophages. [Abstract]2022 Aug;168(8). PMID: 35920812 -
J Dairy Sci
Protective effects of whey protein hydrolysate on Bifidobacterium animalis ssp. lactis Probio-M8 during freeze-drying and storage. [Abstract]2022 Sep;105(9):7308-7321. PMID: 35931487 -
Toxicol Appl Pharmacol
Human trophoblast organoids for improved prediction of placental ABC transporter-mediated drug transport. [Abstract]2024 Nov:492:117112. PMID: 39326791 -
Toxicol Lett
SOD2 promotes the expression of ABCC2 through lncRNA CLCA3p and improves the detoxification capability of liver cells. [Abstract]2020 Jul 1;327:9-18. PMID: 32201199
Solvent & Solubility
DMSO : 100 mg/mL (217.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (284 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Yang T, et al. A novel nonradioactive CFDA assay to monitor the cellular immune response in myeloid leukemia. Immunobiology. 2013 Apr;218(4):548-53. [Content Brief]
[2]. Card SD, et al. Assessment of fluorescein-based fluorescent dyes for tracing Neotyphodium endophytes in planta. Mycologia. 2013 Jan-Feb;105(1):221-9. [Content Brief]
[3]. Fang X, et al. Bone marrow-derived endothelial progenitor cells are involved in aneurysm repair in rabbits. J Clin Neurosci. 2012 Sep;19(9):1283-6. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1721 mL | 10.8604 mL | 21.7207 mL | 54.3018 mL |
| 5 mM | 0.4344 mL | 2.1721 mL | 4.3441 mL | 10.8604 mL | |
| 10 mM | 0.2172 mL | 1.0860 mL | 2.1721 mL | 5.4302 mL | |
| 15 mM | 0.1448 mL | 0.7240 mL | 1.4480 mL | 3.6201 mL | |
| 20 mM | 0.1086 mL | 0.5430 mL | 1.0860 mL | 2.7151 mL | |
| 25 mM | 0.0869 mL | 0.4344 mL | 0.8688 mL | 2.1721 mL | |
| 30 mM | 0.0724 mL | 0.3620 mL | 0.7240 mL | 1.8101 mL | |
| 40 mM | 0.0543 mL | 0.2715 mL | 0.5430 mL | 1.3575 mL | |
| 50 mM | 0.0434 mL | 0.2172 mL | 0.4344 mL | 1.0860 mL | |
| 60 mM | 0.0362 mL | 0.1810 mL | 0.3620 mL | 0.9050 mL | |
| 80 mM | 0.0272 mL | 0.1358 mL | 0.2715 mL | 0.6788 mL | |
| 100 mM | 0.0217 mL | 0.1086 mL | 0.2172 mL | 0.5430 mL |
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