8-BuS-AMP
Based on 1 Customer Validation
8-BuS-AMP is a NTPDase1 inhibitor and a CD73/CD39 inhibitor, with an IC50 of 35 μM and a Ki value of 0.292 μM against human NTPDase1; its Ki values against human CD73 and CD39 are 1.19 μM and 0.847 μM, respectively. 8-BuS-AMP binds to the substrate-binding pockets of NTPDase1 and CD73 to effectively block the conversion of ATP and AMP to adenosine, thereby enhancing the activation and proliferation of human peripheral T lymphocytes. 8-BuS-AMP possesses excellent enzymatic hydrolysis resistance and metabolic stability, resists hydrolysis by multiple NTPDase subtypes, and shows no activity against P2Y1 and P2Y12 receptors. 8-BuS-AMP can be used in purinergic signaling pathway and cancer-related studies.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.90%
- CAS 番号: 344402-39-7
- 分子式: C14H22N5O7PS
- 分子量:435.39
-
保管条件:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
生物活性
-
化学情報
-
溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
-
Help & FAQs
生物活性
製品説明
体外実験
8-BuS-AMP (100 μM; 10 min) impairs recombinant human NTPDase1's ability to inhibit ADP-induced platelet aggregation, but does not itself induce or inhibit platelet aggregation, indicating in vitro inactivity towards platelet P2Y1 and P2Y12 receptors[2].
8-BuS-AMP (1 μM; 0, 20, 40, 60 min) is highly metabolically stable in human and mouse liver microsomes, with half-lives of 462 min and 182 min, respectively[3].
8-BuS-AMP (1-100 μM; 1 h) concentration-dependently inhibits nucleotide hydrolysis in human synovial fluid, reducing adenosine formation from both ε-ATP and ε-AMP[3].
8-BuS-AMP (10-100 μM; 3 days, added daily) concentration-dependently enhances activation (via increased CD25 expression) and proliferation of human primary CD4+ and CD8+ T lymphocytes without reducing cell viability[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
体内実験
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
-
CAS 番号 344402-39-7
-
性状 Solid
-
分子量 435.39
-
分子式 C14H22N5O7PS
-
Color White to off-white
-
SMILES
O[C@H]1[C@@H](O)[C@H](N2C(N=CN=C3N)=C3N=C2SCCCC)O[C@@H]1COP(O)(O)=O
-
輸送条件
Room temperature in continental US; may vary elsewhere.
-
保管条件
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
体外:
H2O : 50 mg/mL (114.84 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
-
データシート (278 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
取扱説明書 (2659 KB)
参考文献
[1]. Baqi Y, et al. Development of Anthraquinone Derivatives as Ectonucleoside Triphosphate Diphosphohydrolase (NTPDase) Inhibitors With Selectivity for NTPDase2 and NTPDase3. Front Pharmacol. 2020;11:1282. Published 2020 Aug 27. [Content Brief]
[2]. Lecka J, et al. 8-BuS-ATP derivatives as specific NTPDase1 inhibitors. Br J Pharmacol. 2013;169(1):179-196. [Content Brief]
[3]. Bi C, et al. Synthesis, Characterization, Interactions, and Immunomodulatory Function of Ectonucleotidase CD39/CD73 Inhibitor 8-Butylthioadenosine 5'-Monophosphate. ACS Pharmacol Transl Sci. 2025;8(5):1401-1415. Published 2025 Apr 3. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.2968 mL | 11.4840 mL | 22.9679 mL | 57.4198 mL |
| 5 mM | 0.4594 mL | 2.2968 mL | 4.5936 mL | 11.4840 mL | |
| 10 mM | 0.2297 mL | 1.1484 mL | 2.2968 mL | 5.7420 mL | |
| 15 mM | 0.1531 mL | 0.7656 mL | 1.5312 mL | 3.8280 mL | |
| 20 mM | 0.1148 mL | 0.5742 mL | 1.1484 mL | 2.8710 mL | |
| 25 mM | 0.0919 mL | 0.4594 mL | 0.9187 mL | 2.2968 mL | |
| 30 mM | 0.0766 mL | 0.3828 mL | 0.7656 mL | 1.9140 mL | |
| 40 mM | 0.0574 mL | 0.2871 mL | 0.5742 mL | 1.4355 mL | |
| 50 mM | 0.0459 mL | 0.2297 mL | 0.4594 mL | 1.1484 mL | |
| 60 mM | 0.0383 mL | 0.1914 mL | 0.3828 mL | 0.9570 mL | |
| 80 mM | 0.0287 mL | 0.1435 mL | 0.2871 mL | 0.7177 mL | |
| 100 mM | 0.0230 mL | 0.1148 mL | 0.2297 mL | 0.5742 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
8-BuS-AMP 関連分類
HY-173447 関連分類
Powered by Bioz