BTS
Based on 3 publication(s) in Google Scholar
BTS (N-Benzyl-p-toluenesulfonamide) is a potent and selective inhibitor of skeletal muscle myosin II subfragment 1 (S1) ATPase activity, with an IC50s of ~5 µM for actin- and Ca2+-stimulated myosin S1 ATPase. BTS specifically inhibits the contraction of fast skeletal muscle fibers.
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- 純度: 99.79%
- CAS 番号: 1576-37-0
- 分子式: C14H15NO2S
- 分子量:261.34
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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MedChemExpress（MCE）の使用を引用している文献 BTS
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生物活性
IC50: ~5 µM (skeletal muscle myosin II S1 ATPase)
BTS (2-12 μM) inhibits the Ca2+-stimulated ATPase activity of myosin S1 in the absence of actin, with an IC50 of ~5 µM[1].
BTS (2-20 μM) reversibly inhibits the gliding motility rate of heavy meromyosin (HMM)[1].
BTS (100 μM) releases myosin from actin in the presence of ADP[1].
BTS (0-20 μM) reversibly inhibits isometric Ca2+-activated tension in skinned skeletal muscle fibres from rabbit and frog, with IC50s of ~3 µM and 1 µM, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 1576-37-0
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性状 Solid
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分子量 261.34
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分子式 C14H15NO2S
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Color White to off-white
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SMILES
O=S(NCC1=CC=CC=C1)(C2=CC=C(C)C=C2)=O
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別名
N-Benzyl-p-toluenesulfonamide; N-Tosylbenzylamine
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (3)
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Journal Impact Factor
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Most Recent
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Nat Commun
Tamoxifen prolongs survival and alleviates symptoms in mice with fatal X-linked myotubular myopathy. [Abstract]2018 Nov 19;9(1):4848. PMID: 30451843 -
Cell Rep Med
2025 Nov 18;6(11):102455. PMID: 41223854 -
ACS Appl Mater Interfaces
Enhanced and Extended Ophthalmic Drug Delivery by pH-Triggered Drug-Eluting Contact Lenses with Large-Pore Mesoporous Silica Nanoparticles. [Abstract]2023 Apr 19;15(15):18630-18638. PMID: 37023369
溶剤 & 溶解度
DMSO : 125 mg/mL (478.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 0.1 mg/mL (0.38 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (9.57 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (9.57 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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データシート (272 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.8264 mL | 19.1322 mL | 38.2643 mL | 95.6608 mL |
| 5 mM | 0.7653 mL | 3.8264 mL | 7.6529 mL | 19.1322 mL | |
| 10 mM | 0.3826 mL | 1.9132 mL | 3.8264 mL | 9.5661 mL | |
| 15 mM | 0.2551 mL | 1.2755 mL | 2.5510 mL | 6.3774 mL | |
| 20 mM | 0.1913 mL | 0.9566 mL | 1.9132 mL | 4.7830 mL | |
| 25 mM | 0.1531 mL | 0.7653 mL | 1.5306 mL | 3.8264 mL | |
| 30 mM | 0.1275 mL | 0.6377 mL | 1.2755 mL | 3.1887 mL | |
| 40 mM | 0.0957 mL | 0.4783 mL | 0.9566 mL | 2.3915 mL | |
| 50 mM | 0.0765 mL | 0.3826 mL | 0.7653 mL | 1.9132 mL | |
| 60 mM | 0.0638 mL | 0.3189 mL | 0.6377 mL | 1.5943 mL | |
| 80 mM | 0.0478 mL | 0.2392 mL | 0.4783 mL | 1.1958 mL | |
| 100 mM | 0.0383 mL | 0.1913 mL | 0.3826 mL | 0.9566 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
BTS 関連分類
HY-16690 関連分類
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