ファストグリーン FCF
Based on 1 publication(s) in Google Scholar
Fast Green FCF is a sea green triarylmethane food dye, with absorption maximum ranging from 622 to 626 nm. Fast Green FCF inhibits α-synuclein aggregation, as well as Aβ and P2X4 receptor, and TLR4/Myd88/NF-κB. Fast Green FCF is widely used as a staining agent like quantitative stain for histones at alkaline pH after acid extraction of DNA, and as a protein stain in electrophoresis. Fast Green FCF improves cognitive impairment, depression, relieves pain allergies, and promotes reproductive function.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.95%
- CAS 番号: 2353-45-9
- 分子式: C37H34N2Na2O10S3
- 分子量:808.85
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保管条件:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 Fast Green FCF
Moreα-synuclein アイソフォーム固有の製品をすべて表示
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生物活性
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α-synuclein Aggregation |
Fast Green FCF (molar ratios of HEWL/FCF: 1 to 0.01-1 to 1, 10 days) suppresses the generation of lysozyme fibrils in a concentration-dependent manner and exhibits disaggregating activity against the preformed/existing lysozyme fibrils[2].
Fast Green FCF (100 μg/mL-1 mg/mL) inhibits synaptic activity in rat hippocampal interneurons, with half-maximum effective concentration of ~200 μg/mL (250 μM)[3].
Fast Green FCF (5-100 μM, 24 h) inhibits α-synuclein aggregation, disassembles mature fibrils and protects against amyloid-induced neurotoxicity in PC12 cells[4].
Fast green FCF (molar ratios of Aβ40 to FGF of 3:1-1:3) alleviates the cytotoxicity induced by Aβ40 fibrillogenesis in PC12 cells[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:PC12 cells with α-syn fibrils
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Concentration:5, 25, 50, 100 μM
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Incubation Time:24 h
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Result:Reduced the cytotoxicity, and the cell viability respectively reached 66.2% (5 μM) and 72.7% (25 μM).
Increased cell viability, reaching 76.4% (50 μM) and 89.0% (100 μM), respectively.
Fast Green FCF (10-100 mg/kg, i.p., once daily, 30 min before surgery) prevents postoperative cognitive dysfunction via the downregulation of the P2X4 receptor in mice[6].
Fast Green FCF (100 mg/kg, i.p., daily for 7 days) attenuates lipopolysaccharide-induced depressive-like behavior and downregulates TLR4/Myd88/NF-κB signal pathway in the mouse hippocampus[7].
Fast Green FCF (30-100 mg/kg, i.p., daily for 9 days) alleviates pain hypersensitivity and down-regulates the levels of spinal P2X4 expression and pro-inflammatory cytokines in a rodent inflammatory pain model[8].
Fast Green FCF (0.017-0.041 gm per kg body weight, p.o., 42 days) causes a significant decrease in testicular weight, tubular diameter, sperm density, and sperm motility[9].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Aβ-induced cognitive impairment mice[5]
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Dosage:30, 100 mg/kg
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Administration:Intraperitoneal injection (i.p.), 30 min prior to each trial
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Result:Did not alter the recognition index.
Prevented the Aβ42-induced recognition impairment at 100 mg/kg.
Prevented the Aβ42-induced impairment of spatial learning at 100 mg/kg.
reduced the Aβ42-induced memory impairment.
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化学情報
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CAS 番号 2353-45-9
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性状 Solid
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分子量 808.85
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分子式 C37H34N2Na2O10S3
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Color Dark green to black
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SMILES
O=S(C1=CC(C/[N+](CC)=C2C=C/C(C=C/2)=C(C3=CC=C(N(CC)CC4=CC=CC(S(=O)(O[Na])=O)=C4)C=C3)\C5=CC=C(O)C=C5S(=O)(O[Na])=O)=CC=C1)([O-])=O
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別名
Fast Green FCF; FD&C Green No. 3; Food green 3; C.I. 42053
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
溶剤 & 溶解度
H2O : 10 mg/mL (12.36 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (286 KB)
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SDS (644 KB)
- English - EN (644 KB)
- Français - FR (644 KB)
- Deutsch - DE (644 KB)
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- Swedish - SV (644 KB)
- Italian - IT (644 KB)
- Korean - KR (644 KB)
- Portuguese - PT (644 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Mittal A, et al. Batch and bulk removal of a triarylmethane dye, Fast Green FCF, from wastewater by adsorption over waste materials. J Hazard Mater. 2009 Apr 30;163(2-3):568-77. [Content Brief]
[2]. How SC, et al. Examining the inhibitory potency of food additive fast green FCF against amyloid fibrillogenesis under acidic conditions. Food Funct. 2016 Dec 7;7(12):4898-4907. [Content Brief]
[3]. van Hooft JA. Fast Green FCF (Food Green 3) inhibits synaptic activity in rat hippocampal interneurons. Neurosci Lett. 2002 Feb 1;318(3):163-5. [Content Brief]
[4]. Wang F, et al. The food additive fast green FCF inhibits α-synuclein aggregation, disassembles mature fibrils and protects against amyloid-induced neurotoxicity. Food Funct. 2021 Jun 21;12(12):5465-5477. [Content Brief]
[5]. Liu F, et al. Fast green FCF inhibits Aβ fibrillogenesis, disintegrates mature fibrils, reduces the cytotoxicity, and attenuates Aβ-induced cognitive impairment in mice. Int J Biol Macromol. 2021 Feb 15;170:33-41. [Content Brief]
[6]. Liu J, et al. Fast green FCF prevents postoperative cognitive dysfunction via the downregulation of the P2X4 receptor in mice. Int Immunopharmacol. 2023 Aug;121:110462. [Content Brief]
[7]. Yang J, et al. Fast Green FCF Attenuates Lipopolysaccharide-Induced Depressive-Like Behavior and Downregulates TLR4/Myd88/NF-κB Signal Pathway in the Mouse Hippocampus. Front Pharmacol. 2019 May 8;10:501. [Content Brief]
[8]. Xu F, et al. Fast Green FCF Alleviates Pain Hypersensitivity and Down-Regulates the Levels of Spinal P2X4 Expression and Pro-inflammatory Cytokines in a Rodent Inflammatory Pain Model. Front Pharmacol. 2018 May 23;9:534. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.2363 mL | 6.1816 mL | 12.3632 mL | 30.9081 mL |
| 5 mM | 0.2473 mL | 1.2363 mL | 2.4726 mL | 6.1816 mL | |
| 10 mM | 0.1236 mL | 0.6182 mL | 1.2363 mL | 3.0908 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Fast Green FCF 関連分類
HY-D0914 関連分類
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