ACE TFA
Based on 1 Customer Validation
ACE TFA is a low-background, photostable fluorophore with nanomolar binding affinity for the Okra RNA aptamer. ACE TFA enables clear visualization of mRNA in live bacteria and mammalian cells, including tracking the trafficking of mRNA to stress granules and dual-color super-resolution imaging of RNA in live cells. (Ex=488 nm, Em=555 nm).
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.47%
- 分子式: C22H27F3N4O4
- 分子量:468.47
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保管条件:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
製品説明
体外実験
ACE TFA (10 nM-10 μM; 3-10 min) forms a highly stable, bright green fluorescent complex with the Okra RNA aptamer, exhibiting nanomolar binding affinity, high quantum yield, low magnesium dependence, potassium independence, and pH stability in vitro[1].
ACE TFA (0.5-2 μM; 10 min at 37°C, up to 1200 s) produces significantly brighter green fluorescence than reference fluorogenic ligands in live HEK293T cells, with exceptional photostability exceeding that of EGFP during long-term continuous imaging when bound to Okra[1].
ACE TFA (0.5 μM; 10 min at 37°C) enables real-time, long-term imaging of mRNA trafficking to and dynamics of stress granules in live HeLa cells when bound to Okra-tagged ACTB mRNA[1].
ACE TFA (1 μM; 10 min at 37°C) supports high-resolution live-cell SIM imaging of subcellular RNA localization, including detailed visualization of RNA distributions in stress granules when bound to the Okra RNA aptamer[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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性状 Solid
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分子量 468.47
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分子式 C22H27F3N4O4
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SMILES
O=C(/C(C#N)=C\C1=CC2=C(N(CCC2)CCC3)C3=C1)NCCOCCN.OC(C(F)(F)F)=O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
溶剤 & 溶解度
体外:
DMSO : 100 mg/mL (213.46 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1346 mL | 10.6730 mL | 21.3461 mL | 53.3652 mL |
| 5 mM | 0.4269 mL | 2.1346 mL | 4.2692 mL | 10.6730 mL | |
| 10 mM | 0.2135 mL | 1.0673 mL | 2.1346 mL | 5.3365 mL | |
| 15 mM | 0.1423 mL | 0.7115 mL | 1.4231 mL | 3.5577 mL | |
| 20 mM | 0.1067 mL | 0.5337 mL | 1.0673 mL | 2.6683 mL | |
| 25 mM | 0.0854 mL | 0.4269 mL | 0.8538 mL | 2.1346 mL | |
| 30 mM | 0.0712 mL | 0.3558 mL | 0.7115 mL | 1.7788 mL | |
| 40 mM | 0.0534 mL | 0.2668 mL | 0.5337 mL | 1.3341 mL | |
| 50 mM | 0.0427 mL | 0.2135 mL | 0.4269 mL | 1.0673 mL | |
| 60 mM | 0.0356 mL | 0.1779 mL | 0.3558 mL | 0.8894 mL | |
| 80 mM | 0.0267 mL | 0.1334 mL | 0.2668 mL | 0.6671 mL | |
| 100 mM | 0.0213 mL | 0.1067 mL | 0.2135 mL | 0.5337 mL |
ACE TFA 関連分類
HY-D2348A 関連分類
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