Coelenterazine TFA
Based on 6 publication(s) in Google Scholar
Coelenterazine TFA is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. Renilla luciferase and substrate Coelenterazine TFA has been used as the bioluminescence donor in bioluminescence resonance energy transfer (BRET) to detect protein-protein interactions. Coelenterazine TFA is a superoxide anion-sensitive chemiluminescent probe and it can also be used in chemiluminescent detection of peroxynitrite (Ex/Em = 429/466 nm).
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.14%
- 分子式: C28H22F3N3O5
- 分子量:537.49
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保管条件:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 Coelenterazine TFA
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生物活性
製品説明
体外実験
HCT-8 control cells, transiently expressing Renilla luciferase (RLuc), showed low bioluminescence due to P-glycoprotein–mediated efflux transport of Coelenterazine TFA. By comparison, transiently expressing RLuc HCT-8 cells, wherein P-glycoprotein was down-regulated with shRNAi, showed high bioluminescence[3].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Measurement of free radical production[5].
1. The measurements are performed in 0.1 M potassium phosphate buffer, pH 7.0.
2. Coelenterazine TFA (2.5 μM) is added to the buffer immediately prior
to measurements.
3. Add 2 mg/mL NADH.
4. At room temperature using a luminometer.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
体内実験
Coelenterazine (2 mg/kg) TFA is injected intravenously.
After 5 minutes, the mice are fixed and imaged for 3 to 5 minutes.
All mice demonstrated very high Rluc activity at the primary site with the majority of mice simultaneously showing metastases to inguinal ILNs[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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性状 Solid
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分子量 537.49
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分子式 C28H22F3N3O5
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Color Light yellow to yellow
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SMILES
O=C1C(CC2=CC=C(O)C=C2)=NC3=C(CC4=CC=CC=C4)NC(C5=CC=C(O)C=C5)=CN31.FC(F)(C(O)=O)F
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (6)
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Journal Impact Factor
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Most Recent
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ACS Sens
Secretory Bioluminescent Reporter Sensor for Functional Assessment of Glucocorticoids In Vitro and In Vivo. [Abstract]2026 Jun 26. PMID: 42358115 -
Plant Cell Environ
A Rapid and Reversible Molecular "Switch" Regulating Protein Expression in Chlamydomonas reinhardtii. [Abstract]2025 Jun;48(6):3913-3924. PMID: 39838873 -
Prostate Cancer Prostatic Dis
Effects of dietary omega-3 fatty acids on orthotopic prostate cancer progression, tumor associated macrophages, angiogenesis and T-cell activation-dependence on GPR120. [Abstract]2022 Sep;25(3):539-546. PMID: 35075215 -
FASEB J
2025 Jun 15;39(11):e70624. PMID: 40444459 -
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溶剤 & 溶解度
体外:
DMSO : 100 mg/mL (186.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (292 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Markova SV, et al, Coelenterazine-dependent luciferases. Biochemistry (Mosc). 2015 Jun;80(6):714-32. [Content Brief]
[2]. Lucas M, et al. Coelenterazine is a superoxide anion-sensitive chemiluminescent probe: its usefulness in the assay of respiratory burst in neutrophils. Anal Biochem. 1992 Nov 1;206(2):273-7. [Content Brief]
[3]. Pichler A, et al. In vivo RNA interference-mediated ablation of MDR1 P-glycoprotein. Clin Cancer Res. 2005 Jun 15;11(12):4487-94. [Content Brief]
[4]. Volk-Draper LD, et al. Novel model for basaloid triple-negative breast cancer: behavior in vivo and response to therapy. Neoplasia. 2012 Oct;14(10):926-42. [Content Brief]
[5]. Raha S, et al. Control of oxygen free radical formation from mitochondrial complex I: roles for protein kinase A and pyruvate dehydrogenase kinase. Free Radic Biol Med. 2002 Mar 1;32(5):421-30. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8605 mL | 9.3025 mL | 18.6050 mL | 46.5125 mL |
| 5 mM | 0.3721 mL | 1.8605 mL | 3.7210 mL | 9.3025 mL | |
| 10 mM | 0.1860 mL | 0.9302 mL | 1.8605 mL | 4.6512 mL | |
| 15 mM | 0.1240 mL | 0.6202 mL | 1.2403 mL | 3.1008 mL | |
| 20 mM | 0.0930 mL | 0.4651 mL | 0.9302 mL | 2.3256 mL | |
| 25 mM | 0.0744 mL | 0.3721 mL | 0.7442 mL | 1.8605 mL | |
| 30 mM | 0.0620 mL | 0.3101 mL | 0.6202 mL | 1.5504 mL | |
| 40 mM | 0.0465 mL | 0.2326 mL | 0.4651 mL | 1.1628 mL | |
| 50 mM | 0.0372 mL | 0.1860 mL | 0.3721 mL | 0.9302 mL | |
| 60 mM | 0.0310 mL | 0.1550 mL | 0.3101 mL | 0.7752 mL | |
| 80 mM | 0.0233 mL | 0.1163 mL | 0.2326 mL | 0.5814 mL | |
| 100 mM | 0.0186 mL | 0.0930 mL | 0.1860 mL | 0.4651 mL |
Coelenterazine TFA 関連分類
HY-18743A 関連分類
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