DiAzKs
Based on 1 publication(s) in Google Scholar
DiAzKs (H-L-Photo-lysine) is a diazirine-containing lysine amino acid and is a photo-cross-linker. DiAzKs can site-selective incorporated into proteins and is used to crosslink protein-protein interactions in vitro and in living cells. DiAzKs acts as a UV light-activated photo-crosslinking probe.
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- 純度 : 99.93%
- CAS 番号: 1253643-88-7
- 分子式: C11H20N4O4
- 分子量:272.30
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保管条件:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 DiAzKs
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生物活性
製品説明
体外実験
Photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine post-translational modifications (PTMs), including 'readers' and 'erasers' of histone modifications[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
化学情報
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CAS 番号 1253643-88-7
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性状 Solid
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分子量 272.30
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分子式 C11H20N4O4
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Color White to yellow
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SMILES
N[C@@H](CCCCNC(OCCC1(C)N=N1)=O)C(O)=O
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別名
H-L-Photo-lysine
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
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Nat Biotechnol
2021 Mar;39(3):347-356. PMID: 33077962
溶剤 & 溶解度
体外:
H2O : 5 mg/mL (18.36 mM; Need ultrasonic)
DMSO : 2 mg/mL (7.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (270 KB)
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SDS (406 KB)
- English - EN (406 KB)
- Français - FR (406 KB)
- Deutsch - DE (406 KB)
- Norwegian - NO (406 KB)
- Español - ES (406 KB)
- Swedish - SV (406 KB)
- Italian - IT (406 KB)
- Korean - KR (406 KB)
- Portuguese - PT (406 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Ai HW, et al. Probing protein-protein interactions with a genetically encoded photo-crosslinking amino acid. Chembiochem. 2011 Aug 16;12(12):1854-7. [Content Brief]
[2]. Chatterjee A, et al. Efficient viral delivery system for unnatural amino acid mutagenesis in mammalian cells. Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. [Content Brief]
[3]. Yang T, et al. Photo-lysine captures proteins that bind lysine post-translational modifications. Nat Chem Biol. 2016 Feb;12(2):70-2. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 3.6724 mL | 18.3621 mL | 36.7242 mL | 91.8105 mL |
| 5 mM | 0.7345 mL | 3.6724 mL | 7.3448 mL | 18.3621 mL | |
| H2O | 10 mM | 0.3672 mL | 1.8362 mL | 3.6724 mL | 9.1811 mL |
| 15 mM | 0.2448 mL | 1.2241 mL | 2.4483 mL | 6.1207 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
DiAzKs 関連分類
HY-D0853 関連分類
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