Ginisortamab
Based on 1 Customer Validation
Ginisortamab (UCB6114) is a fully human IgG4P anti Gremlin-1 monoclonal antibody, with mean IC50 values of 8.2 nM and 9 nM against human and mouse gremlin-1, respectivly. Ginisortamab inhibits gremlin-1 antagonism of BMP signaling pathways. Ginisortamab has the potential for the research of gastrointestinal (GI) tumors.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度 : 99.05%
- CAS 番号: 2390147-17-6
- 分子量:147.56 kDa
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
生物活性
製品説明
Isotype
Human IgG4 kappa
Recommend Isotype Controls
Species Reactivity
Human
IC50 & Target
GREM1/Gremlin
体外実験
Ginisortamab (0.1-1000 nM) inhibits both human and mouse gremlin-1 in a concentration-dependent manner, with mean IC50 values of 8.2 nM and 9 nM, respectively[2].
The geometric mean affinities (equilibrium dissociation constant [KD]) of Ginisortamab for human and mouse gremlin-1 are 87 pM and 61 pM, respectively[2].
Ginisortamab (150 nM, 30-120 min) inhibits gremlin-1 antagonism of BMP signaling pathways in human CRC cell and fibroblasts lines[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
臨床実験
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
遺伝子ID
アクセッション番号
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Human IgG4 kappa
アプリケーション
ELISA, FACS, Functional assay
Verified Bioactivity
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Immobilized Human Gremlin/GREM1 Protein, His Tag can bind Ginisortamab. The EC50 for this effect is 10.22 ng/mL. -
Flow cytometric analysis of 1X106 SH-SY5Y cells labeling GREM1 with Ginisortamab (HY-P99643, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 for an hour at 4℃. AF488-conjugated Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG4 kappa Isotype Control (HY-P99003, blue) was used as the isotype control.
化学情報
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CAS 番号 2390147-17-6
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性状 Liquid
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分子量 147.56 kDa
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Color Colorless to light yellow
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SMILES
[Ginisortamab]
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別名
UCB6114
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輸送条件
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
プロトコル
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
純度とドキュメンテーション
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データシート (257 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)