HD-TAC7
Based on 1 Customer Validation
HD-TAC7 is a potent PROTAC HDAC degrader with IC50 values of 3.6 μM, 4.2 μM and 1.1 μM for HDAC1, HDAC2 and HDAC3, respectively. HD-TAC7 can decreases NF-κB p65 in RAW 264.7 macrophages. HD-TAC7 can be used for the research of inflammatory diseases like asthma and chronic obstructive pulmonary disease (COPD).
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 98.53%
- CAS 番号: 2978763-95-8
- 分子式: C33H32FN7O7
- 分子量:657.65
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
PROTACs アイソフォーム固有の製品をすべて表示
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生物活性
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HDAC1 3.6 μM (IC50) |
HDAC2 4.2 μM (IC50) |
HDAC3 1.1 μM (IC50) |
HD-TAC7 (10 μM; 24 hours) induces an increase of H3K27 acetylation in RAW 264.7 macrophages[1].
HD-TAC7 (10 μM; 2-48 hours) induces HDAC3 degradation reaching the maximal effect at 6h and lasted at least 48h[1].
HD-TAC7 (1 and 10 μM; 24 hours) downregulates NF-κB p65 in LPS-treated RAW 264.7 macrophages[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 2978763-95-8
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性状 Solid
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分子量 657.65
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分子式 C33H32FN7O7
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Color Light yellow to green yellow
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SMILES
NC1=CC(F)=CC=C1NC(C2=CC=C(C=C2)NC(CCCCNC(CNC3=C4C(N(C(C4=CC=C3)=O)C5CCC(NC5=O)=O)=O)=O)=O)=O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
DMSO : 100 mg/mL (152.06 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.63 mg/mL (4.00 mM); Clear solution
This protocol yields a clear solution of ≥ 2.63 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (26.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
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データシート (271 KB)
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SDS (254 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5206 mL | 7.6028 mL | 15.2057 mL | 38.0141 mL |
| 5 mM | 0.3041 mL | 1.5206 mL | 3.0411 mL | 7.6028 mL | |
| 10 mM | 0.1521 mL | 0.7603 mL | 1.5206 mL | 3.8014 mL | |
| 15 mM | 0.1014 mL | 0.5069 mL | 1.0137 mL | 2.5343 mL | |
| 20 mM | 0.0760 mL | 0.3801 mL | 0.7603 mL | 1.9007 mL | |
| 25 mM | 0.0608 mL | 0.3041 mL | 0.6082 mL | 1.5206 mL | |
| 30 mM | 0.0507 mL | 0.2534 mL | 0.5069 mL | 1.2671 mL | |
| 40 mM | 0.0380 mL | 0.1901 mL | 0.3801 mL | 0.9504 mL | |
| 50 mM | 0.0304 mL | 0.1521 mL | 0.3041 mL | 0.7603 mL | |
| 60 mM | 0.0253 mL | 0.1267 mL | 0.2534 mL | 0.6336 mL | |
| 80 mM | 0.0190 mL | 0.0950 mL | 0.1901 mL | 0.4752 mL | |
| 100 mM | 0.0152 mL | 0.0760 mL | 0.1521 mL | 0.3801 mL |
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