Lodapolimab
Based on 1 Customer Validation
Lodapolimab (LY3300054) is a fully human IgG1λ anti-PD-L1 monoclonal antibody, with an EC50 of 0.075 nM against hPD-L1 and an EC50 of 0.085 nM against cynomolgus monkey PD-L1. Lodapolimab induces naive T cell activation, enhances the T cell activation effect of anti-CTLA4 antibodies, promotes anti-tumor alloreactivity, regulates innate immune pathways, and induces characteristics of adaptive immune activation. Lodapolimab can be used in research related to high-grade serous ovarian cancer and other solid tumors.
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研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.71%
- CAS 番号: 2118349-31-6
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
Human IgG1 lambda
Human
B7-H1/PD-L1/CD274
Lodapolimab (0.001-100 nM; 2 h) binds specifically to hPD-L1 and cynomolgus monkey PD-L1 with high affinity (with EC50 values of 0.075 nM and 0.085 nM, respectively), and does not bind to mouse PD-L1 or other related immunoglobulin superfamily proteins[1].
Lodapolimab (pre-incubated for 1 h) potently blocks the interactions of PD-L1 with its cognate receptors PD-1 (IC50 = 0.95 nM) and CD80 (IC50 = 2.4 nM)[1].
Lodapolimab specifically binds to PD-L1 expressed on the surface of tumor cell lines (NCI-H292, HCC827, OV79), and does not bind to PD-L1-negative tumor cells (A204)[1].
Lodapolimab (0.01-100 nM; 6 h) enhances T cell activation in a PD-1/PD-L1-dependent manner, which is verified by the increased NFAT-driven luciferase activity in a co-culture system of PD-L1+ CHO-K1 cells and PD-1-expressing Jurkat cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Lodapolimab (10 mg/kg; i.p.; once weekly; 4 doses total) significantly enhances T cell-mediated anti-tumor activity in established NSG mouse lung cancer models[1].
Lodapolimab (10 mg/kg; intraperitoneal injection; once weekly; 4 doses total) induces potent anti-tumor activity and systemic T cell activation in the NOG mouse ovarian cancer model[1].
Lodapolimab (10 mg/kg; intraperitoneal injection; once weekly; 3 doses total) induces significant anti-tumor activity and T-cell inflammatory gene signatures in NSG mouse lung cancer models[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) (female, 6-7 weeks of age)[1]
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Dosage:10 mg/kg
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Administration:i.p.; weekly; 4 doses
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Result:Induced significant tumor inhibition (p < 0.001) compared to human IgG1-treated controls.
Sustained suppression of tumor volume over 30 days post-implant.\nSignificantly enhanced the anti-tumor activity of infused human T cells, resulting in potent tumor growth inhibition (p < 0.001) compared to human IgG1-treated controls.
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Animal Model:NOD.Cg-Prkdcscid Il2rgtm1Sug/JicTac (NOG) (female, 15-17 weeks of age, engrafted with cord blood-derived CD34+ human hematopoietic stem cells)[1]
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Dosage:10 mg/kg
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Administration:i.p.; weekly; 4 doses
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Result:Resulted in significant tumor growth inhibition (p < 0.001) compared to human IgG1-treated controls.
Increased absolute human T cell counts by day 46.
Elevated the CD8:CD4 T cell ratio (p = 0.0001 by day 34).
Increased the frequency of PD-1+ CD4+ (p = 0.0009 by day 34) and PD-1+ CD8+ (p = 0.0008 by day 34) T cells, indicative of T cell activation.
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Animal Model:NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) (female, 15-17 weeks of age, engrafted with fetal liver-derived CD34+ human hematopoietic stem cells)[1]
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Dosage:10 mg/kg
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Administration:i.p.; weekly; 3 doses
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Result:Resulted in significant tumor growth inhibition (p < 0.001) compared to human IgG1-treated controls.
Upregulated genes associated with T cell infiltration and activation (CD3E, CD8B, CD4, PRF1, GZMB, TBX21), myeloid cell infiltration (ITGAM, ITGAX, CD14, CD68), co-stimulatory/co-inhibitory receptors (TNFRSF9, ICOS, PDCD1), cytokines (IFNG, IL2, CCL5), and antigen presentation (HLA-A, HLA-B) in tumor tissue.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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Human IgG1 lambda
ELISA, FACS, Functional assay
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Immobilized PD-L1 Protein, Human (HEK293, HY-P73361) can bind Lodapolimab. The EC50 for this effect is 6.358 ng/mL.
化学情報
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CAS 番号 2118349-31-6
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性状 Liquid
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Color Colorless to light yellow
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SMILES
[Lodapolimab]
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別名
LY3300054
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輸送条件
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
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データシート (263 KB)
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SDS (251 KB)
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- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
参考文献
[1]. Li Y, et al. Discovery and preclinical characterization of the antagonist anti-PD-L1 monoclonal antibody LY3300054. Journal for immunotherapy of cancer. 2018 Apr 30;6(1):31. [Content Brief]
[3]. Do KT, et al. Immune modulating activity of the CHK1 inhibitor prexasertib and anti-PD-L1 antibody LY3300054 in patients with high-grade serous ovarian cancer and other solid tumors. Cancer immunology, immunotherapy : CII. 2021 Oct;70(10):2991-3000. [Content Brief]
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
Lodapolimab 関連分類
HY-P99709 関連分類
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