PD 90780
Based on 2 publication(s) in Google Scholar
PD 90780 is a non peptide antagonist of nerve growth factor (NGF). PD 90780 interacts with NGF, prevents NGF binds with p75NTR. PD 90780 inhibits NGF-p75NTR interaction with IC50s of 23.1 and 1.8 μM in PC12 cells and PC12nnr5 cells, respectively .
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- 純度 : 98.83%
- CAS 番号: 77422-99-2
- 分子式: C19H14N4O4
- 分子量:362.34
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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生物活性
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化学情報
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溶剤 & 溶解度
- プロトコル
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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MedChemExpress（MCE）の使用を引用している文献 PD 90780
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生物活性
製品説明
IC50 & Target
IC50: 23.1 µM (NGF-p75NTR, PC12 cells), 1.8 µM (NGF-p75NTR, PC12nnr5 cells)[1]
体外実験
PD 90780 (0.1-100 μM; 2 h) affects 125I-BDNF binds to truncated p75NTR[1]. PD 90780 (100 μM; 2 h) inhibits 125I-NGF cross-linking to p75NTR[1]. PD 90780 (0-300 μM; 2 h) decreases TrkA phosphorylation resulting from NGF binding with a dose of 300 μM[1]. PD 90780 (0.1-100 μM; 2 h) inhibits NGF-p75NTR interaction with IC50 values of 23.1 μM and 1.8 μM in PC12 cells and PC12nnr5 cells, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
化学情報
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CAS 番号 77422-99-2
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性状 Solid
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分子量 362.34
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分子式 C19H14N4O4
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Color Light yellow to yellow
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SMILES
CN1C2=CC(C(O)=O)=NN2C(C3=CC(NC(C4=CC=CC=C4)=O)=CC=C13)=O
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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J Adv Res
Astragaloside II, a natural saponin, facilitates remyelination in demyelination neurological diseases via p75NTR receptor mediated β-catenin/Id2/MBP signaling axis in oligodendrocyte precursor cells. [Abstract]2025 Apr 19:S2090-1232(25)00273-5. PMID: 40258474 -
J Hazard Mater
BDE-47 induced PC-12 cell differentiation via TrkA downstream pathways and caused the loss of hippocampal neurons in BALB/c mice. [Abstract]2022 Jan 15;422:126850. PMID: 34419847
溶剤 & 溶解度
体外:
DMSO : 31.25 mg/mL (86.24 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
プロトコル
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PC12 NGF-Induced Neuronal Differentiation Culture
PC12 cells are a rat adrenal pheochromocytoma clonal line that responds to NGF by stopping proliferation and extending branching neurite-like processes; after longer NGF exposure, cells develop long processes and neuronal-like ultrastructural and functional features. NGF-induced differentiation is read out mainly by neurite outgrowth, reduced proliferation, microtubule assembly, and neuronal differentiation-associated proteins such as MAPs, tau, GAP-43, and synapsin-1.
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PC12 NGF-induced neuronal-like differentiation
PC12 cells are a rat adrenal pheochromocytoma-derived clonal cell line that responds to nerve growth factor by stopping proliferation and extending neurites, producing a sympathetic neuron-like phenotype used to study neuronal differentiation and neurite outgrowth. NGF acts through TrkA-dependent signaling, and neurite outgrowth is associated with ERK/Akt signaling, microtubule organization, neuronal-marker expression, and increased electrophysiological neuronal features such as sodium-channel density. The main assay readout is morphological differentiation, usually measured as the percentage of neurite-bearing cells, neurite length, neurite number, or total neurite length per cell. Additional readouts include GAP-43, tyrosine hydroxylase, βIII-tubulin, neurofilament, synapsin I, synaptophysin, ERK phosphorylation, Akt phosphorylation, and sodium-channel current density.
純度とドキュメンテーション
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データシート (272 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Colquhoun A , et al. Differential activity of the nerve growth factor (NGF) antagonist PD90780 [7-(benzolylamino)-4,9-dihydro-4-methyl-9-oxo-pyrazolo[5,1-b]quinazoline-2-carboxylic acid] suggests altered NGF-p75NTR interactions in the presence of TrkA. J Pharmacol Exp Ther. 2004 Aug;310(2):505-11. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7598 mL | 13.7992 mL | 27.5984 mL | 68.9960 mL |
| 5 mM | 0.5520 mL | 2.7598 mL | 5.5197 mL | 13.7992 mL | |
| 10 mM | 0.2760 mL | 1.3799 mL | 2.7598 mL | 6.8996 mL | |
| 15 mM | 0.1840 mL | 0.9199 mL | 1.8399 mL | 4.5997 mL | |
| 20 mM | 0.1380 mL | 0.6900 mL | 1.3799 mL | 3.4498 mL | |
| 25 mM | 0.1104 mL | 0.5520 mL | 1.1039 mL | 2.7598 mL | |
| 30 mM | 0.0920 mL | 0.4600 mL | 0.9199 mL | 2.2999 mL | |
| 40 mM | 0.0690 mL | 0.3450 mL | 0.6900 mL | 1.7249 mL | |
| 50 mM | 0.0552 mL | 0.2760 mL | 0.5520 mL | 1.3799 mL | |
| 60 mM | 0.0460 mL | 0.2300 mL | 0.4600 mL | 1.1499 mL | |
| 80 mM | 0.0345 mL | 0.1725 mL | 0.3450 mL | 0.8624 mL |
PD 90780 関連分類
HY-110166 関連分類
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