Polymyxin B1
Based on 1 publication(s) in Google Scholar
Polymyxin B1 is a potent antimicrobial lipopeptide first derived from Bacilus polymyxa. Polymyxin B1 is the major component in Polymyxin B (HY-A0248). Polymyxin B1 can induce lysis of bacterial cells through interaction with their membranes. Polymyxin B1 has the potential for multidrug-resistant Gram-negative bacterial infections treatment.
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- 純度: 98.90%
- CAS 番号: 4135-11-9
- 分子式: C56H98N16O13
- 分子量:1203.48
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保管条件:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
MedChemExpress（MCE）の使用を引用している文献 Polymyxin B1
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生物活性
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HK-2 | CC50 |
>128 μM
Compound: Polymyxin B
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Cytotoxicity against human HK-2 cells assessed as reduction in cell viability incubated for 1 hr by resazurin based microplate reader method
Cytotoxicity against human HK-2 cells assessed as reduction in cell viability incubated for 1 hr by resazurin based microplate reader method
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[PMID: 35311294] |
| J774.1 | IC50 |
8.5 μM
Compound: Polymyxin B
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Antiinflammatory activity in mouse J774.1 cells assessed as inhibition of LPS/TNFgamma-induced NO production after 48 hrs by Griess assay
Antiinflammatory activity in mouse J774.1 cells assessed as inhibition of LPS/TNFgamma-induced NO production after 48 hrs by Griess assay
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[PMID: 28836430] |
| Vero | CC50 |
71 μg/mL
Compound: Polymyxin B1
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Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay
Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay
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[PMID: 29412662] |
Polymyxin B1 has antimicrobial activity that againsts Pseudomonas aeruginosa ATCC 27853, Acinetobacter baumannii ATCC BAA 747, Klebsiella pneumoniae ATCC 13883, P. aeruginosa 9019, A. baumannii 1261 and K. pneumoniae VM9 isolates with MIC values of 4 μg/mL, 2 μg/mL, 2 μg/mL, 4 μg/mL, 4 μg/mL and 2 μg/mL, respectively[3].
Polymyxin B1 strongly inhibits protein synthesis in yeast, and in E. coli and S. aureus[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
化学情報
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CAS 番号 4135-11-9
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性状 Solid
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分子量 1203.48
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分子式 C56H98N16O13
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Color White to off-white
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配列
{Dab(6-methyl-1-oxooctyl)}-Thr-{Dab}-{Dab}-{Dab}-{d-Phe}-Leu-{Dab}-{Dab}-Thr (Lactam bridge: Dab4-Thr10)
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シーケンスの短縮
{Dab(6-methyl-1-oxooctyl)}-T-{Dab}-{Dab}-{Dab}-{d-Phe}-L-{Dab}-{Dab}-T (Lactam bridge: Dab4-Thr10)
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Structure Classification
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Initial Source
Bacilus polymyxa
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Publications (1)
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Journal Impact Factor
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Most Recent
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ACS Appl Bio Mater
A Stepwise Targeting and Antibacterial Strategy by Leukocyte Membrane-Based Conjugated Oligomer Nanoparticles. [Abstract]2023 Jun 19;6(6):2487-2495. PMID: 37289442
溶剤 & 溶解度
1 M HCl : 10 mg/mL (8.31 mM; Need ultrasonic and warming)
1 M HCl : 10 mg/mL (8.31 mM; Need ultrasonic and warming)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (281 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Kwa AL, et al. Pharmacokinetics of polymyxin B1 in patients with multidrug-resistant Gram-negative bacterial infections. Diagn Microbiol Infect Dis. 2008 Feb;60(2):163-7. [Content Brief]
[2]. Berglund NA, et al. Interaction of the antimicrobial peptide polymyxin B1 with both membranes of E. coli: a molecular dynamics study. PLoS Comput Biol. 2015 Apr 17;11(4):e1004180. [Content Brief]
[3]. Tam VH, et al. In vitro potency of various polymyxin B components. Antimicrob Agents Chemother. 2011 Sep;55(9):4490-1. [Content Brief]
[4]. Alonso MA, et al. Compounds affecting membranes that inhibit protein synthesis in yeast. Antimicrob Agents Chemother. 1979 Dec;16(6):750-6. [Content Brief]
[5]. Sivanesan S, et al. Pharmacokinetics of the Individual Major Components of Polymyxin B and Colistin in Rats. J Nat Prod. 2017 Jan 27;80(1):225-229. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| 1 M HCl / 1 M HCl | 1 mM | 0.8309 mL | 4.1546 mL | 8.3092 mL | 20.7731 mL |
| 5 mM | 0.1662 mL | 0.8309 mL | 1.6618 mL | 4.1546 mL |
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