Pozelimab
Based on 1 Customer Validation
Pozelimab (REGN3918) is a fully human IgG4 anti-C5 monoclonal antibody. Pozelimab binds to C5 and C5 variants with high affinity and blocks complement-mediated hemolysis. Pozelimab can be used for the research of complement-mediated diseases.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 99.42%
- CAS 番号: 2096328-94-6
- 分子量:144.78 kDa
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保管条件:
Please store the product under the recommended conditions in the Certificate of Analysis.
生物活性
Human IgG4 kappa
Human
Complement C5
Pozelimab binds with human C5 and cynomolgus monkey C5 with Kd values of 262 pM and 8.55 nM, respectively at 37˚C, pH7.4[1]. Pozelimab blocks classic pathway (CP)-mediated hemolysis in normal human and cynomolgus monkey serum with IC50 values of 2.8 and 20 nM, respectively at 37˚C, pH7.4[1]. Pozelimab blocks alternative pathway (AP)-mediated hemolysis in normal human and cynomolgus monkey serum with IC50 values of 26 and 8.9 nM, respectively at 37˚C, pH7.4[1]. Pozelimab binds to human C5, cynomolgus monkey C5, human R885C C5 and human C5b,6 with Kd values of 0.189, 2.73, 0.422, 0.465 and 0.137 nM respectively at 25˚C, pH7.4[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Cynomolgus monkey[1]
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Dosage:15 mg/kg
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Administration:Intravenous injection; 15 mg/kg, once
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Result:Showed a a greater exposure to monkeys compared with H4H12161P and H4H12170P.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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Human IgG4 kappa
ELISA, FACS, Functional assay
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Flow cytometric analysis of 1X106 HepG2 cells with Pozelimab (HY-P99786, red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Human IgG H&L (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG4 kappa Isotype Control (HY-P99003, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
化学情報
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CAS 番号 2096328-94-6
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性状 Liquid
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分子量 144.78 kDa
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Color Colorless to light yellow
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SMILES
[Pozelimab]
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別名
REGN3918
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輸送条件
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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保管条件
Please store the product under the recommended conditions in the Certificate of Analysis.
純度とドキュメンテーション
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データシート (259 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
参考文献
Calculators
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)