TBBS-CPT

TBBS-CPT undergoes GSH-mediated cleavage to release CPT and TBBS in a cell-free PBS buffer system, and exhibits highly sensitive and selective fluorescent responses to GSH at concentrations ranging from 0 to 100 μM^[1].
TBBS-CPT (5.0 μM; 0-60 min) is selectively taken up by biotin receptor-positive 4T1 cancer cells and preferentially localizes to the mitochondria of 4T1 cells^[1].
TBBS-CPT produces both type I (O₂•^-) and type II (¹O₂) reactive oxygen species synchronously upon white light irradiation, and displays equivalent reactive oxygen species generation efficiency under normoxic and hypoxic environments^[1].

TBBS-CPT (0-15 μM; 25 mW/cm²) exhibits potent synergistic chemo-photodynamic cytotoxicity against 4T1 cells, with an IC₅₀ value of 1.54 μM under normoxic conditions and 2.05 μM under hypoxic conditions; its cytotoxicity decreases against biotin-pretreated 4T1 cells (IC₅₀ = 13.47 μM), and its toxicity to NIH-3T3 normal fibroblasts is negligible^[1].
TBBS-CPT (5.0 μM; imaged at 0-10 min post-white light irradiation) induces time-dependent mitochondrial dysfunction in 4T1 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.