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製品番号: HY-W319295

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TEMPOL-H

TEMPOL-H is the reduced hydroxylamine form of the stable nitroxide radical Tempol (HY-100561), and serves as the active metabolite of OT-551 (HY-W556870). TEMPOL-H acts as a potent antioxidant and free radical scavenger. TEMPOL-H protects retinal pigment epithelial cells from acute light-induced damage, and protects mice from lethal whole-body radiation. TEMPOL-H inhibits lens opacification in mice, prevents glutathione loss in rat lenses under stress, blocks protein leakage in rhesus monkey lenses under stress, and maintains the ³H-choline accumulation capacity of the lens. TEMPOL-H can be used in studies related to light-induced retinal pigment epithelial degeneration and age-related cataract.

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研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号: 3637-10-3
分子式: C₉H₁₉NO₂
分子量:173.25

保管条件:

Please store the product under the recommended conditions in the Certificate of Analysis.

TEMPOL-H 構造式

CAS 番号: 3637-10-3

価格（税別）

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

体外実験

TEMPOL-H inhibits photo-oxidative damage induced by short-wavelength light in lipofuscin fluorophore-loaded retinal pigment epithelial cells in vitro^[1].
Tempol-H (4 mM; 18-20 h) significantly preserves ³H-choline accumulation in organ-cultured Sprague Dawley rat lenses damaged by induction with 250 μM H₂O₂^[3].
Tempol-H (4 mM; 24 h) inhibits 1.0 mM H₂O₂-induced lens opacification in Sprague Dawley rat organ cultures maintained for 24 hours^[3].
Tempol-H (4 mM; 18 h) prevents glutathione loss in lenses from Sprague Dawley rats treated with 1.5 mM H₂O₂ during 18 h organ culture^[3].
Tempol-H (4 mM; 5 h) inhibits oxidative aggregation of water-soluble bovine lens crystallins treated with flavin mononucleotide (FMN) and visible light^[3].
Tempol-H (1-4 mM; 9 days) inhibits xylose (HY-N0537)-induced opacification of rhesus monkey lenses during 9 days of organ culture^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

体内実験

TEMPOL-H (100 mg/kg; i.p.; single administration 30 min before light exposure) protects RPE cells in the inferior retina of Sprague-Dawley rats against acute light-induced degeneration^[1].
Tempol-H (325 mg/kg; i.p.; single administration; 5-10 minutes prior to irradiation) acts as an in vivo radioprotector in female C3H mice against lethality induced by whole-body irradiation, with a dose modification factor of 1.3^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Sprague-Dawley (6-7 weeks of age; light-induced retinal pigment epithelium degeneration model)^[1]
Dosage:

100 mg/kg
Administration:

i.p.; single dose 30 minutes prior to light exposure
Result:

Showed no significant difference in retinal pigment epithelium cell nuclei count between light-exposed inferior hemisphere eyes and non-light-exposed control eyes.
Demonstrated a significantly higher retinal pigment epithelium damage index than 100 mg/kg OT-551 in the superior hemisphere of light-exposed eyes.

Animal Model:

C3H mice (female, 60-80 days old, 20-30 g, whole-body γ-irradiation with ¹³⁷Cs source at 1 Gy/min dose rate)^[2]
Dosage:

325 mg/kg
Administration:

i.p.; single dose; 5-10 minutes pre-irradiation
Result:

Provided radioprotection against whole-body radiation-induced lethality at 30 days: near-complete survival was observed at 8 Gy and 9 Gy, with protection seen up to 13 Gy.
Achieved a dose modification factor (DMF) of 1.3.

化学情報

CAS 番号 3637-10-3
分子量 173.25
分子式 C₉H₁₉NO₂
SMILES

OC1CC(C)(C)N(O)C(C)(C)C1
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

Please store the product under the recommended conditions in the Certificate of Analysis.

純度とドキュメンテーション

取扱説明書 (2659 KB)

参考文献

[1]. Tanito M, et al. Protection of retinal pigment epithelium by OT-551 and its metabolite TEMPOL-H against light-induced damage in rats. Experimental eye research. 2010 Jul;91(1):111-4. [Content Brief]

[2]. Hahn SM, et al. Evaluation of the hydroxylamine Tempol-H as an in vivo radioprotector. Free radical biology & medicine. 2000 Mar 15;28(6):953-8.

[3]. Zigler JS, et al. Tempol-H inhibits opacification of lenses in organ culture. Free radical biology & medicine. 2003 Nov 15;35(10):1194-202.

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100 mg

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