TN-16
Based on 1 Customer Validation
TN-16 is a Microtubule polymerization inhibitor. TN-16 induces G2/M cell cycle arrest, metaphase mitotic arrest and Apoptotic cell death in cells, and blocks late Autophagic flux by inhibiting autophagosome-lysosome fusion. TN-16 suppresses tumor growth in syngeneic mouse breast cancer models. TN-16 can be used in research related to neuroblastoma, cervical cancer, breast cancer and other tumors.
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- 純度: 99.78%
- CAS 番号: 33016-12-5
- 分子式: C19H18N2O2
- 分子量:306.36
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保管条件:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
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生物活性
TN-16 (1 μM; 72 h) potently reduces the viability of neuroblastoma SH-SY5Y cells, with an EC50 of 68 nM[1].
TN-16 exhibits cytotoxicity against cervical cancer HeLa cells[1].
TN-16 (200 nM-1 μM; 16 h) induces G2/M phase cell cycle arrest in neuroblastoma SH-SY5Y cells[1].
TN-16 (1 μM; 16 h) causes only an extremely slight change in the ratio of tubulin polymerization/depolymerization in neuroblastoma SH-SY5Y cells[1].
TN-16 (1 μM-10 μM; 61 min) inhibits the polymerization of purified tubulin in a dose-dependent manner, and a strong inhibitory effect is observed at a concentration of 10 μM[1].
TN-16 (1.25 µmol/L; 24-48 h) enhances apoptosis activation, which is manifested as increased cleavage levels of Caspase-3 and PARP in Atg7-knockdown HCT-116 (Bax+/-) cells[2].
Incubation with TN-16 (10 μM; up to 60 min) at 30°C for up to 60 min inhibits the assembly of porcine brain tubulin in vitro, as determined by turbidity measurement at 350 nm[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:human neuroblastoma SH-SY5Y cells
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Concentration:1 μM
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Incubation Time:72 h
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Result:Exhibited cytotoxic activity with an EC50 of 68 nM.
Reduced cell viability to 34.7% of control.
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Cell Line:human neuroblastoma SH-SY5Y cells
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Concentration:200 nM-1 μM
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Incubation Time:16 h
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Result:Induced a distinct G2/M cell cycle arrest at both tested concentrations.
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Cell Line:Atg7-knockdown human HCT-116 (Bax+/-) colon cancer cells
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Concentration:1.25 µmol/L
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Incubation Time:24, 48 h
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Result:Induced enhanced cleavage of caspase-3 and PARP in Atg7-knockdown cells relative to autophagy-proficient scrambled control cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Crl:CD1-Foxn1nu (5- to 6-week-old female; orthotopic syngeneic breast cancer model via transplantation of 1×106 4T1 mouse mammary carcinoma cells into the mammary fat pad)[2]
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Dosage:1 mg/kg
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Administration:i.p.; daily; 11 days
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Result:Reduced tumour volume to ~270 mm3 by day 11 post-treatment initiation versus ~520 mm3 in controls.
Lowered average harvested tumour weight significantly compared to controls.
Increased levels of cleaved PARP and cleaved caspase-3 in tumour tissue lysates.
Induced accumulation of p62 and increased LC3-II turnover, indicating blockade of autophagic flux and enhanced apoptosis.
化学情報
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CAS 番号 33016-12-5
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性状 Solid
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分子量 306.36
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分子式 C19H18N2O2
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Color White to off-white
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SMILES
O=C(NC(C/1=O)CC2=CC=CC=C2)C1=C(C)/NC3=CC=CC=C3
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
溶剤 & 溶解度
DMSO : 50 mg/mL (163.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (279 KB)
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取扱説明書 (2659 KB)
参考文献
[1]. Foroutan A, et al. Identification of novel aza-analogs of TN-16 as disrupters of microtubule dynamics through a multicomponent reaction. Eur J Med Chem. 2023;245(Pt 1):114895. [Content Brief]
[2]. Hasanain M, et al. Microtubule disrupting agent-mediated inhibition of cancer cell growth is associated with blockade of autophagic flux and simultaneous induction of apoptosis. Cell Prolif. 2020;53(4):e12749. [Content Brief]
[3]. Arai T, et al. Inhibition of microtubule assembly in vitro by TN-16, a synthetic antitumor drug. FEBS Lett. 1983;155(2):273-276. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2641 mL | 16.3207 mL | 32.6413 mL | 81.6033 mL |
| 5 mM | 0.6528 mL | 3.2641 mL | 6.5283 mL | 16.3207 mL | |
| 10 mM | 0.3264 mL | 1.6321 mL | 3.2641 mL | 8.1603 mL | |
| 15 mM | 0.2176 mL | 1.0880 mL | 2.1761 mL | 5.4402 mL | |
| 20 mM | 0.1632 mL | 0.8160 mL | 1.6321 mL | 4.0802 mL | |
| 25 mM | 0.1306 mL | 0.6528 mL | 1.3057 mL | 3.2641 mL | |
| 30 mM | 0.1088 mL | 0.5440 mL | 1.0880 mL | 2.7201 mL | |
| 40 mM | 0.0816 mL | 0.4080 mL | 0.8160 mL | 2.0401 mL | |
| 50 mM | 0.0653 mL | 0.3264 mL | 0.6528 mL | 1.6321 mL | |
| 60 mM | 0.0544 mL | 0.2720 mL | 0.5440 mL | 1.3601 mL | |
| 80 mM | 0.0408 mL | 0.2040 mL | 0.4080 mL | 1.0200 mL | |
| 100 mM | 0.0326 mL | 0.1632 mL | 0.3264 mL | 0.8160 mL |
TN-16 関連分類
HY-119357 関連分類
Powered by Bioz
- TN-16
- 33016-12-5
- TN16
- TN 16
- Microtubule/Tubulin
- Apoptosis
- Autophagy
- microtubule assembly
- G2/M cell cycle arrest
- human cervical cancer HeLa cells
- syngeneic mouse breast cancer model
- human neuroblastoma SH-SY5Y cells
- porcine tubulin
- α-tubulin acetylation
- autophagic flux
- apoptotic cell death
- colchicine-sensitive site
- Inhibitor
- inhibitor
- inhibit