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KM04416, an isothiazolone derivative, is a potent glycerol-3-phosphate dehydrogenase (GPD2) inhibitor. KM04416 significantly inhibits PNT1A cell proliferation.

For research use only. We do not sell to patients.

KM04416 Chemical Structure

KM04416 Chemical Structure

CAS No. : 26530-26-7

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 132 In-stock
Solution
10 mM * 1 mL in DMSO USD 132 In-stock
Solid
5 mg USD 120 In-stock
10 mg USD 190 In-stock
25 mg USD 380 In-stock
50 mg USD 605 In-stock
100 mg USD 960 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

KM04416 Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

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Description

KM04416, an isothiazolone derivative, is a potent glycerol-3-phosphate dehydrogenase (GPD2) inhibitor. KM04416 significantly inhibits PNT1A cell proliferation[1][2].

In Vitro

KM04416 (20 µM; 48 h) has a growth inhibitory effect on several cancer cell lines[1].
KM04416 (10 µM; 72 h) significantly inhibits PNT1A cell proliferation[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

KM04416 Related Antibodies

Cell Viability Assay[1]

Cell Line: Several cancer cell lines including MDA-MB-231 and AsPC-1 as well as Huh-7, HepG2, and SK-HEP-1 cells
Concentration: 20 µM
Incubation Time: 48 h
Result: Had a growth inhibitory effect.

Cell Proliferation Assay[2]

Cell Line: PNT1A cell
Concentration: 10 µM
Incubation Time: 72 h
Result: Significantly inhibited PNT1A cell proliferation.
Molecular Weight

249.29

Formula

C12H11NO3S
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=C(OCC)C1=CC=C(N2SC=CC2=O)C=C1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (200.57 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.0114 mL 20.0570 mL 40.1139 mL
5 mM 0.8023 mL 4.0114 mL 8.0228 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (10.03 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.0114 mL 20.0570 mL 40.1139 mL 100.2848 mL
5 mM 0.8023 mL 4.0114 mL 8.0228 mL 20.0570 mL
10 mM 0.4011 mL 2.0057 mL 4.0114 mL 10.0285 mL
15 mM 0.2674 mL 1.3371 mL 2.6743 mL 6.6857 mL
20 mM 0.2006 mL 1.0028 mL 2.0057 mL 5.0142 mL
25 mM 0.1605 mL 0.8023 mL 1.6046 mL 4.0114 mL
30 mM 0.1337 mL 0.6686 mL 1.3371 mL 3.3428 mL
40 mM 0.1003 mL 0.5014 mL 1.0028 mL 2.5071 mL
50 mM 0.0802 mL 0.4011 mL 0.8023 mL 2.0057 mL
60 mM 0.0669 mL 0.3343 mL 0.6686 mL 1.6714 mL
80 mM 0.0501 mL 0.2507 mL 0.5014 mL 1.2536 mL
100 mM 0.0401 mL 0.2006 mL 0.4011 mL 1.0028 mL
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KM04416 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

KM0441626530-26-7KM 04416KM-04416OthersIsothiazoloneglycerol-3-phosphate dehydrogenaseGPD2PNT1AproliferationMDA-MB-231AsPC-1Huh-7HepG2SK-HEP-1Inhibitorinhibitorinhibit

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