TAT
Based on 1 Customer Validation
TAT (YGRKKRRQRRR) is derived from the transactivator of transcription (TAT) of human immunodeficiency virus-1 (HIV-1) and is a cell-penetrating peptide. TAT can increase the yields and the solubility of heterologous proteins.
For research use only. We do not sell to patients.
- Purity: 99.92%
- CAS No.: 191936-91-1
- Formula: C64H118N32O14
- Molecular Weight:1559.83
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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HIV-1 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
>100 μM
Compound: p-02
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Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 24 hrs by CCK-8 assay
Cytotoxicity against human A549 cells assessed as reduction in cell viability measured after 24 hrs by CCK-8 assay
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[PMID: 35452935] |
| BEAS-2B | IC50 |
>100 μM
Compound: p-02
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Cytotoxicity against human BEAS-2B cells assessed as reduction in cell viability measured after 24 hrs by CCK-8 assay
Cytotoxicity against human BEAS-2B cells assessed as reduction in cell viability measured after 24 hrs by CCK-8 assay
|
[PMID: 35452935] |
| HepG2 | IC50 |
>100 μM
Compound: p-02
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Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 24 hrs by CCK-8 assay
Cytotoxicity against human HepG2 cells assessed as reduction in cell viability measured after 24 hrs by CCK-8 assay
|
[PMID: 35452935] |
| L02 | IC50 |
>100 μM
Compound: p-02
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Cytotoxicity against human L02 cells assessed as reduction in cell viability measured after 24 hrs by CCK-8 assay
Cytotoxicity against human L02 cells assessed as reduction in cell viability measured after 24 hrs by CCK-8 assay
|
[PMID: 35452935] |
TAT (YGRKKRRQRRR) represents amino acids 47-57 of the human immunodeficiency virus type I (HIV-1) TAT protein. The positively charged protein transduction domain of the HIV-1 TAT protein (TAT-PTD; residues 47-57 of TAT) rapidly translocates across the plasma membrane of living cells. TAT can cross the hydrophobic lipid bilayer of a cell membrane simply by passive diffusion[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 191936-91-1
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Appearance Solid
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Molecular Weight 1559.83
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Formula C64H118N32O14
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Color White to off-white
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Synonyms
TAT(47-57); HIV-1 TAT protein (47-57)
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Sequence
Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg
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Sequence Shortening
YGRKKRRQRRR
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Structure Classification
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Initial Source
HIV-1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H2O : ≥ 50 mg/mL (32.05 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 0.6411 mL | 3.2055 mL | 6.4110 mL | 16.0274 mL |
| 5 mM | 0.1282 mL | 0.6411 mL | 1.2822 mL | 3.2055 mL | |
| 10 mM | 0.0641 mL | 0.3205 mL | 0.6411 mL | 1.6027 mL | |
| 15 mM | 0.0427 mL | 0.2137 mL | 0.4274 mL | 1.0685 mL | |
| 20 mM | 0.0321 mL | 0.1603 mL | 0.3205 mL | 0.8014 mL | |
| 25 mM | 0.0256 mL | 0.1282 mL | 0.2564 mL | 0.6411 mL | |
| 30 mM | 0.0214 mL | 0.1068 mL | 0.2137 mL | 0.5342 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
TAT Related Classifications
HY-P0281 Related Classifications
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