3-Methoxycatechol
Based on 1 Customer Validation
3-Methoxycatechol is intermediate for the antivascular agents combretastatin A-1 (HY-121993) and combretastatin B-1. 3-Methoxycatechol promotes esophageal carcinogenesis in rats models.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity: 99.98%
- CAS No.: 934-00-9
- 화학식: C7H8O3
- 분자량:140.14
-
보관:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
용액&용해도
- 순도&문서
- References
-
Help & FAQs
Biological Activity
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| MCF7 | GI50 |
27.55 μM
Compound: 3c
|
Cytotoxicity against human MCF7 cells assessed as cell growth inhibition after 48 hrs by SRB assay
Cytotoxicity against human MCF7 cells assessed as cell growth inhibition after 48 hrs by SRB assay
|
[PMID: 28041801] |
| TK-10 | GI50 |
51.63 μM
Compound: 3c
|
Cytotoxicity against human TK10 cells assessed as cell growth inhibition after 48 hrs by SRB assay
Cytotoxicity against human TK10 cells assessed as cell growth inhibition after 48 hrs by SRB assay
|
[PMID: 28041801] |
| UACC-62 | GI50 |
23.64 μM
Compound: 3c
|
Cytotoxicity against human UACC62 cells assessed as cell growth inhibition after 48 hrs by SRB assay
Cytotoxicity against human UACC62 cells assessed as cell growth inhibition after 48 hrs by SRB assay
|
[PMID: 28041801] |
Chemical Information
-
CAS No. 934-00-9
-
Appearance <42.8°C Solid,>42.8°C Liquid
-
분자량 140.14
-
화학식 C7H8O3
-
Color Colorless to off-white
-
SMILES
COC1=CC=CC(=C1O)O
-
선적
Room temperature in continental US; may vary elsewhere.
-
보관
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
용액&용해도
DMSO : 200 mg/mL (1427.16 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 100 mg/mL (713.58 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (35.68 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (35.68 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
순도&문서
-
Data Sheet (277 KB)
-
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
-
Handling Instructions (2659 KB)
References
[1]. Tao Y, et al., Altering toluene 4-monooxygenase by active-site engineering for the synthesis of 3-methoxycatechol, methoxyhydroquinone, and methylhydroquinone. J Bacteriol. 2004 Jul;186(14):4705-13. [Content Brief]
[2]. Hirose M, et al., Effects of sodium nitrite and catechol, 3-methoxycatechol, or butylated hydroxyanisole in combination in a rat multiorgan carcinogenesis model. Cancer Res. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 7.1358 mL | 35.6791 mL | 71.3582 mL | 178.3956 mL |
| 5 mM | 1.4272 mL | 7.1358 mL | 14.2716 mL | 35.6791 mL | |
| 10 mM | 0.7136 mL | 3.5679 mL | 7.1358 mL | 17.8396 mL | |
| 15 mM | 0.4757 mL | 2.3786 mL | 4.7572 mL | 11.8930 mL | |
| 20 mM | 0.3568 mL | 1.7840 mL | 3.5679 mL | 8.9198 mL | |
| 25 mM | 0.2854 mL | 1.4272 mL | 2.8543 mL | 7.1358 mL | |
| 30 mM | 0.2379 mL | 1.1893 mL | 2.3786 mL | 5.9465 mL | |
| 40 mM | 0.1784 mL | 0.8920 mL | 1.7840 mL | 4.4599 mL | |
| 50 mM | 0.1427 mL | 0.7136 mL | 1.4272 mL | 3.5679 mL | |
| 60 mM | 0.1189 mL | 0.5947 mL | 1.1893 mL | 2.9733 mL | |
| 80 mM | 0.0892 mL | 0.4460 mL | 0.8920 mL | 2.2299 mL | |
| 100 mM | 0.0714 mL | 0.3568 mL | 0.7136 mL | 1.7840 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
3-Methoxycatechol Related Classifications
HY-W116576 Related Classifications
Powered by Bioz