84-B10
Based on 1 publication(s) in Google Scholar
84-B10 is a 3-phenylglutaric acid derivative. 84-B10 inhibits cisplatin (HY-17394) induced tubular ferroptosis. 84-B10 attenuates cisplatin-induced mitochondrial damage and oxidative stress. 84-B10 ameliorates cisplatin-induced acute kidney injury (AKI).
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- Purity : 99.61%
- CAS No.: 698346-43-9
- 화학식: C25H22F3NO5
- 분자량:473.44
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 84-B10
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Biological Activity
제품 설명
In Vitro
84-B10 (10-100 μM; 2 h) inhibits cisplatin-induced tubular epithelial cell ferroptosis in a dose-dependent manner[1].
84-B10 (40 μM; 2 h; TKPT cells) restores cisplatin-induced mitochondrial structural damage and dysfunction[1].
84-B10 (40 μM; 2 h; TKPT cells) attenuates mtROS-induced oxidative stress in cisplatin-induced AKI[1].
84-B10 (40 μM; 2 h; TKPT cells) attenuates cisplatin-induced epithelial cell injury by eliminating mtROS and restoring mitochondrial homeostasis[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:tubular epithelial cell
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Concentration:10, 20, 30, 40, 50, and 100 μM
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Incubation Time:2 hours
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Result:Increased the levels of NRF2, SLC7A11, and GPX4 in a dose-dependent manner.
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Cell Line:TKPT cells
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Concentration:40 μM
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Incubation Time:2 hours
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Result:Increased the levels of OM Porins, IMS Cyt c, IM CVa, IM Core 1, and Matrix CypD in a dose-dependent manner.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male C57BL/6 mice with acute kidney injury[1]
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Dosage:5, 10, and 15 mg/kg
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Administration:intraperitoneal injection
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Result:Decreased the sCr and BUN levels of cisplatin-exposed mice.
Attenuated renal tubules morphological abnormalities in a dose-dependent manner.
Decreased NGAL and KIM-1 levels in a dose-dependent manner.
Decreased the transcription levels of Lcn2 (which encodes NGAL) and Havcr1.
Chemical Information
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CAS No. 698346-43-9
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Appearance Solid
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분자량 473.44
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화학식 C25H22F3NO5
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Color White to off-white
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SMILES
O=C(O)CC(CC(NC1=CC(C(F)(F)F)=CC=C1OC2=CC=C(OC)C=C2)=O)C3=CC=CC=C3
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
용액&용해도
In Vitro:
DMSO : 100 mg/mL (211.22 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.64 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (2.64 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (276 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1122 mL | 10.5610 mL | 21.1220 mL | 52.8050 mL |
| 5 mM | 0.4224 mL | 2.1122 mL | 4.2244 mL | 10.5610 mL | |
| 10 mM | 0.2112 mL | 1.0561 mL | 2.1122 mL | 5.2805 mL | |
| 15 mM | 0.1408 mL | 0.7041 mL | 1.4081 mL | 3.5203 mL | |
| 20 mM | 0.1056 mL | 0.5281 mL | 1.0561 mL | 2.6403 mL | |
| 25 mM | 0.0845 mL | 0.4224 mL | 0.8449 mL | 2.1122 mL | |
| 30 mM | 0.0704 mL | 0.3520 mL | 0.7041 mL | 1.7602 mL | |
| 40 mM | 0.0528 mL | 0.2640 mL | 0.5281 mL | 1.3201 mL | |
| 50 mM | 0.0422 mL | 0.2112 mL | 0.4224 mL | 1.0561 mL | |
| 60 mM | 0.0352 mL | 0.1760 mL | 0.3520 mL | 0.8801 mL | |
| 80 mM | 0.0264 mL | 0.1320 mL | 0.2640 mL | 0.6601 mL | |
| 100 mM | 0.0211 mL | 0.1056 mL | 0.2112 mL | 0.5281 mL |
84-B10 Related Classifications
HY-44307 Related Classifications
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