CMK
Based on 10 publication(s) in Google Scholar
CMK is a RSK2 kinase inhibitor which exhibits similar potency but less chemical stability compared with FMK.
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- Purity: 99.64%
- CAS No.: 821794-90-5
- 화학식: C18H19ClN4O2
- 분자량:358.82
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보관:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
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Immunology/Inflammation Compound Library
HY-L007
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Kinase Inhibitor Library
HY-L009
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MAPK Compound Library
HY-L010
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HY-L025
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Anti-Obesity Compound Library
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HY-L099
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Cysteine Targeted Covalent Library
HY-L153
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Serine/Threonine Kinase Inhibitor Library
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HY-L196
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High-Throughput Bioactive Compound Library
HY-L205
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Posttranslational Modification Library
HY-L226
Publications Citing Use of MedChemExpress (MCE) CMK
More- Mol Cell. 2022 Nov 3;82(21):4033-4048.e7. [Abstract]
- Nat Commun. 2022 Oct 26;13(1):6345. [Abstract]
- EMBO J. 2023 Oct 16;42(20):e114288. [Abstract]
- Cell Chem Biol. 2018 Feb 15;25(2):154-165.e11. [Abstract]
- Elife. 2021 Jan 22:10:e63645. [Abstract]
- J Am Heart Assoc. 2021 Aug 17;10(16):e020554. [Abstract]
- Commun Biol. 2024 Oct 22;7(1):1370. [Abstract]
- mBio. 2025 Feb 7:e0322624. [Abstract]
- Cell Signal. 2025 Apr 28:132:111839. [Abstract]
- bioRxiv. 2023 Dec 15.
Biological Activity
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RSK2 |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HeLa | IC50 |
4 μM
Compound: 4; cmk
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Inhibition of proteasomal turnover of p97-independent ODD-luciferase reporter substrate in human HeLa cells incubated for 120 mins by luciferase reporter gene assay
Inhibition of proteasomal turnover of p97-independent ODD-luciferase reporter substrate in human HeLa cells incubated for 120 mins by luciferase reporter gene assay
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[PMID: 33476933] |
CMK inhibits the growth of Cdc5 (L158G) with IC50 of 36 nM, greater than 30 μM for wild type Cdc5. CMK exhibits a concentration-dependent first cell cycle mitotic arrest in the cdc5-as1 strain with an IC50 of 1.1 μM. CMK inhibition of Cdc5 (L158G) leads to a first cell cycle anaphase arrest and delay in anaphase spindle migration[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 821794-90-5
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Appearance Solid
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분자량 358.82
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화학식 C18H19ClN4O2
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Color Off-white to light brown
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SMILES
NC1=C(C2=NC=N1)C(C3=CC=C(C=C3)C)=C(N2CCCO)C(CCl)=O
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선적
Room temperature in continental US; may vary elsewhere.
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보관
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Publications (10)
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Journal Impact Factor
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Most Recent
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Mol Cell
Yeast Mediator facilitates transcription initiation at most promoters via a Tail-independent mechanism. [Abstract]2022 Nov 3;82(21):4033-4048.e7. PMID: 36208626 -
Nat Commun
Multi-omics characterization of autophagy-related molecular features for therapeutic targeting of autophagy. [Abstract]2022 Oct 26;13(1):6345. PMID: 36289218 -
EMBO J
Spo13/MEIKIN ensures a Two-Division meiosis by preventing the activation of APC/CAma1 at meiosis I. [Abstract]2023 Oct 16;42(20):e114288. PMID: 37728253 -
Cell Chem Biol
Conserved Lipid and Small-Molecule Modulation of COQ8 Reveals Regulation of the Ancient Kinase-like UbiB Family. [Abstract]2018 Feb 15;25(2):154-165.e11. PMID: 29198567 -
Elife
2021 Jan 22:10:e63645. PMID: 33481703 -
J Am Heart Assoc
Krüppel-Like Factor 15/Interleukin 11 Axis-Mediated Adventitial Remodeling Depends on Extracellular Signal-Regulated Kinases 1 and 2 Activation in Angiotensin II-Induced Hypertension. [Abstract]2021 Aug 17;10(16):e020554. PMID: 34350769 -
Commun Biol
2024 Oct 22;7(1):1370. PMID: 39438667 -
mBio
Inhibition of TIGIT on NK cells improves their cytotoxicity and HIV reservoir eradication potential. [Abstract]2025 Feb 7:e0322624. PMID: 39918313 -
Cell Signal
2025 Apr 28:132:111839. PMID: 40306349 -
용액&용해도
DMSO : 150 mg/mL (418.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.97 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
For Cdc5 kinase assays, Spc72-TAP is isolated from cell extract by pulldown with IgG sepharose or rabbit IgG coupled to M-270 epoxy dynabeads and incubated in kinase buffer (25 mM HEPES, pH 8.0, 60 mM KCl, 15 mM MnCl2, 100 μg/mL BSA, 80 nM microcystin, 1mM DTT, 100 μM 200 μCi/mL [γ-32P]ATP) in the absence or presence of 100 ng purified baculovirus expressed 6xHis-Cdc5. 32P incorporation is visualized on a Typhoon PhosphorImager, and images are processed using ImageQuant software.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
순도&문서
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Data Sheet (280 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7869 mL | 13.9346 mL | 27.8691 mL | 69.6728 mL |
| 5 mM | 0.5574 mL | 2.7869 mL | 5.5738 mL | 13.9346 mL | |
| 10 mM | 0.2787 mL | 1.3935 mL | 2.7869 mL | 6.9673 mL | |
| 15 mM | 0.1858 mL | 0.9290 mL | 1.8579 mL | 4.6449 mL | |
| 20 mM | 0.1393 mL | 0.6967 mL | 1.3935 mL | 3.4836 mL | |
| 25 mM | 0.1115 mL | 0.5574 mL | 1.1148 mL | 2.7869 mL | |
| 30 mM | 0.0929 mL | 0.4645 mL | 0.9290 mL | 2.3224 mL | |
| 40 mM | 0.0697 mL | 0.3484 mL | 0.6967 mL | 1.7418 mL | |
| 50 mM | 0.0557 mL | 0.2787 mL | 0.5574 mL | 1.3935 mL | |
| 60 mM | 0.0464 mL | 0.2322 mL | 0.4645 mL | 1.1612 mL | |
| 80 mM | 0.0348 mL | 0.1742 mL | 0.3484 mL | 0.8709 mL | |
| 100 mM | 0.0279 mL | 0.1393 mL | 0.2787 mL | 0.6967 mL |
CMK Related Classifications
HY-52101 Related Classifications
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