Cat. No.: HY-111621

DC661

Name: DC661
Brand: MedChemExpress
SKU: HY-111621
Price: 132.00 USD
Availability: InStock
Rating: 5 (6 reviews)

6 Cited Publications

고객리뷰

Based on 6 publication(s) in Google Scholar

DC661 is a potent palmitoyl-protein thioesterase 1 (PPT1) inhibitor, inhibits autophagy, and acts as an anti-lysosomal agent. Anti-cancer activity.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity : 99.53%
CAS No.: 1872387-43-3
화학식: C₃₁H₃₉Cl₂N₅
분자량:552.58

보관:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

DC661 Chemical Structure

CAS No.: 1872387-43-3

사이즈

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수량

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (6)
용액&용해도
- In Vitro
- In Vivo
Protocol (3)
순도&문서
References
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

DC661 is a potent palmitoyl-protein thioesterase 1 (PPT1) inhibitor, inhibits autophagy, and acts as an anti-lysosomal agent. Anti-cancer activity^[1].

IC₅₀ & Target

PPT1, autophagy^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
A375P	IC₅₀	0.49 μM Compound: 2; DC661	Cytotoxicity against human A375P cells incubated for 72 hrs by MTT assay Cytotoxicity against human A375P cells incubated for 72 hrs by MTT assay	[PMID: 34298133]

In Vitro

DC661 (0.1 and 10 μM) accumulates autophagic vesicles in melanoma cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Chemical Information

CAS No. 1872387-43-3
Appearance Solid
분자량 552.58
화학식 C₃₁H₃₉Cl₂N₅
Color White to yellow
SMILES

CN(CCCCCCNC1=CC=NC2=CC(Cl)=CC=C12)CCCCCCNC3=CC=NC4=CC(Cl)=CC=C34
선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (6)

All WBCell Imaging/StainingRT-PCRFlow CytometryIn Vivo Efficacy Study

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Journal Impact Factor

Nat Biotechnol

Ras-mutant cancers are sensitive to small molecule inhibition of V-type ATPases in mice. [Abstract]

2022 Dec;40(12):1834-1844. PMID: 35879364
Mol Cell

Lysosomal damage sensing and lysophagy initiation by SPG20-ITCH. [Abstract]

2024 Apr 18;84(8):1556-1569.e10. PMID: 38503285
Nat Chem Biol

Tegavivint triggers TECR-dependent nonapoptotic cancer cell death. [Abstract]

2025 May 26. PMID: 40419770
J Immunother Cancer

Intratumoral PPT1-positive macrophages determine immunosuppressive contexture and immunotherapy response in hepatocellular carcinoma. [Abstract]

2023 Jun;11(6):e006655. PMID: 37385725

DC661 purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2023 Jun;11(6):e006655. [Abstract]

Western blot of MAPK and NF-κB signaling pathways in THP-1-differentiated macrophages treated with the DMSO and DC661.

DC661 purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2023 Jun;11(6):e006655. [Abstract]

qPCR analyses of the indicated genes in DMSO and DC661 treatment groups.

DC661 purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2023 Jun;11(6):e006655. [Abstract]

Flow cytometry analysis of CD80, galectin-9, and CD172a expression in DMSO and DC661 treatment groups.

DC661 purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2023 Jun;11(6):e006655. [Abstract]

Gross appearance of the subcutaneous HCC tumors treated with DC661 (30 mg/kg, i.p.).
Biosci Rep

Identification of PPT1 as a lysosomal core gene with prognostic value in hepatocellular carcinoma. [Abstract]

2023 May 31;43(5):BSR20230067. PMID: 37103469

DC661 purchased from MedChemExpress. Usage Cited in: Biosci Rep. 2023 May 31;43(5):BSR20230067. [Abstract]

Representative images and quantification of colony formation assay. HCC cells were chronically treated with DC661 (2 μM, 10 days) for colony formation assays.
Res Sq

A clinical drug candidate that triggers non-apoptotic cancer cell death. [Abstract]

2025 Feb 11:rs.3.rs-4138879. PMID: 39989975

용액&용해도

In Vitro:

DMSO : 62.5 mg/mL (113.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.8097 mL

9.0485 mL

18.0969 mL

5 mM

0.3619 mL

1.8097 mL

3.6194 mL

10 mM

0.1810 mL

0.9048 mL

1.8097 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (3.76 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

All Cell Biology Signal Pathway Related

Autophagy
Autophagy is a process in which eukaryotic cells use lysosomes to degrade their own cytoplasmic proteins and damaged organelles under the regulation of autophagy related gene (Atg). Microtubule-associated proteins light chain 3 (LC3) is recognized as autophagy marker, which transfers from cytoplasmic LC3 (LC3-I) to membrane type (LC3-II). LC3-II/I ratio could be detected by Western Blot and fluorescence microscopy.
Lysosome and acidic-vesicle live-cell staining
Lysosome and acidic-vesicle live-cell staining detects acidic intracellular compartments by using membrane-permeant acidotropic probes that accumulate in low-pH vesicles, including lysosomes, late endosomes, autolysosomes, and acidic phagosomes. LysoTracker staining is commonly used as an intensity-based readout of acidic lysosomal compartment abundance or enlargement, while acridine orange produces green fluorescence in less concentrated compartments and red fluorescence after concentration-dependent accumulation in acidic vesicular organelles. Loss or reduction of acridine-orange red signal can be used as a readout of lysosomal membrane permeabilization or reduced acidic-vesicle integrity. This protocol is designed for live cultured cells and can be adapted for fluorescence microscopy, high-content imaging, plate-reader readout, or flow cytometry when the selected literature supports the readout. Because these dyes report acidotropic accumulation rather than lysosome identity alone,
Macroautophagy Solutions
Macroautophagy is a conserved lysosome-dependent degradation pathway in which cytoplasmic material is sequestered into double-membrane autophagosomes and delivered to lysosomes for degradation and recycling. The pathway supports cellular homeostasis during nutrient limitation, organelle stress, protein-aggregate accumulation, infection, differentiation, and tissue remodeling by coupling cargo sequestration, autophagosome maturation, lysosomal fusion, and degradation of cargo-derived macromolecules. The core molecular sequence includes initiation by nutrient- and stress-regulated autophagy machinery, autophagosome nucleation, LC3/ATG8-family conjugation to autophagosomal membranes, cargo selection through receptors such as SQSTM1/p62, autophagosome-lysosome fusion, and lysosomal degradation. LC3 was identified as a mammalian homolog of yeast Atg8 that localizes to autophagosomal membranes after processing, and p62/SQSTM1 was shown to connect ubiquitinated cargo with autophagic degradati

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (267 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Rebecca VW, et al. PPT1 Promotes Tumor Growth and Is the Molecular Target of Chloroquine Derivatives in Cancer. ancer Discov. 2019 Feb;9(2):220-229. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.8097 mL	9.0485 mL	18.0969 mL	45.2423 mL
	5 mM	0.3619 mL	1.8097 mL	3.6194 mL	9.0485 mL
	10 mM	0.1810 mL	0.9048 mL	1.8097 mL	4.5242 mL
	15 mM	0.1206 mL	0.6032 mL	1.2065 mL	3.0162 mL
	20 mM	0.0905 mL	0.4524 mL	0.9048 mL	2.2621 mL
	25 mM	0.0724 mL	0.3619 mL	0.7239 mL	1.8097 mL
	30 mM	0.0603 mL	0.3016 mL	0.6032 mL	1.5081 mL
	40 mM	0.0452 mL	0.2262 mL	0.4524 mL	1.1311 mL
	50 mM	0.0362 mL	0.1810 mL	0.3619 mL	0.9048 mL
	60 mM	0.0302 mL	0.1508 mL	0.3016 mL	0.7540 mL
	80 mM	0.0226 mL	0.1131 mL	0.2262 mL	0.5655 mL
	100 mM	0.0181 mL	0.0905 mL	0.1810 mL	0.4524 mL

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DC661

Publications Citing Use of MedChemExpress (MCE) DC661