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Cat. No.: HY-101897

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Fura-2 AM

Name: Fura-2 AM
Brand: MedChemExpress
SKU: HY-101897
Price: 400.00 USD
Availability: InStock
Rating: 5 (9 reviews)

(Synonyms: Fura-2 Acetoxymethyl ester)

9 Cited Publications

고객리뷰

Based on 9 publication(s) in Google Scholar

Fura-2 AM is a membrane permeable, intracellular, UV light-excitable and ratiometric fluorescent Ca²⁺ (calcium ion) indicator. Fura-2 AM crosses cell membranes and is converted to Fura-2 (HY-D0110A) via cellular esterases. Fura-2 AM can be used to detect calcium in cells.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity: 98.53%
CAS No.: 108964-32-5
화학식: C₄₄H₄₇N₃O₂₄
분자량:1001.85

보관:

-20°C, protect from light

* The compound is unstable in solutions, freshly prepared is recommended.

Fura-2 AM Chemical Structure

CAS No.: 108964-32-5

사이즈

가격

재고

수량

Amount: USD 0.00

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- In Vitro
- Emission (Em)
- Excitation (Ex)
Chemical Information
Publications (9)
용액&용해도
- In Vitro
순도&문서
References
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

In Vitro

Guideline (The following is our recommended protocol. This protocol is for reference only and should be modified according to your specific requirements).
Preparation of Stock Solution
Add anhydrous DMSO to Fura-2 AM, pipette gently or vortex until completely dissolved to prepare a 1 mM stock solution.
Cell Loading
2.1 Preparation of Working Solution (prepare freshly before use):
Preheat serum-free or low-serum medium to 37℃, add the above 1 mM stock solution, and mix by gentle inversion to prepare a 1 μM loading working solution.
(Optional) To facilitate uniform intracellular dye entry, 0.02% Pluronic F-127 or 0.1% BSA can be added.
2.2 Loading Incubation:
Transfer the coverslips with neuronal cells to a 35 mm culture dish containing the working solution.
Incubate at 37℃ for 30 minutes in the dark.
2.3 Post-incubation (De-esterification):
Preheat 2 mL of fresh dye-free culture medium.
Take out the coverslips from the loading solution and transfer them into the fresh medium.
Continue incubation at 37℃ for 20–30 minutes in the dark. This step is critical to remove extracellular residual dye and ensure sufficient intracellular hydrolysis of the dye into free Fura-2 acid.
3. Pre-imaging Preparation
3.1 Washing: Gently rinse the coverslips 1–2 times with prewarmed HBSS or imaging buffer (containing Ca2?/Mg2?) to eliminate background dye.
3.2 Mounting: Mount the coverslip onto the imaging chamber and add 1–2 mL of imaging buffer.
3.3 Stabilization: Place the chamber on a thermostatted microscope stage (37℃), equilibrate for 5–10 minutes before baseline recording.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Emission (Em)

505

Excitation (Ex)

336

Chemical Information

CAS No. 108964-32-5
Appearance Liquid (Density: 1.400±0.06 g/cm³)
분자량 1001.85
화학식 C₄₄H₄₇N₃O₂₄
Color Light yellow to green yellow
SMILES

O=C(C1=CN=C(C2=CC3=CC(OCCOC4=CC(C)=CC=C4N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C3O2)O1)OCOC(C)=O
Synonyms

Fura-2 Acetoxymethyl ester
선적

Room temperature in continental US; may vary elsewhere.
보관

-20°C, protect from light

* The compound is unstable in solutions, freshly prepared is recommended.

Publications (9)

All Others

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Journal Impact Factor

Neuron

Piezo1-mediated mechanotransduction in choroid plexus epithelial cells governs ciliogenesis and cerebrospinal fluid homeostasis. [Abstract]

2026 Mar 30:S0896-6273(26)00138-8. PMID: 41916280
EMBO J

TRPML1 suppresses pulmonary fibrosis by limiting collagen and elastin deposition. [Abstract]

2026 Apr;45(7):2182-2209. PMID: 41714729
Sci Rep

Estrogen regulates duodenal calcium absorption and improves postmenopausal osteoporosis by the effect of ERβ on PMCA1b. [Abstract]

2025 May 8;15(1):16053. PMID: 40341576
Purinergic Signal

Antagonism of the ATP-gated P2X7 receptor inhibits the proliferation of hepatocellular carcinoma cells. [Abstract]

2024 Nov 16. PMID: 39549156
Purinergic Signal

Potential role of the P2X7 receptor in the proliferation of human diffused large B-cell lymphoma. [Abstract]

2024 Jun;20(3):273-284. PMID: 37222921

Fura-2 AM purchased from MedChemExpress. Usage Cited in: Purinergic Signal. 2024 Jun;20(3):273-284. [Abstract]

The Ca2+ influx function of P2X7 in OCI-ly3 and SU-DHL-4 was validated by measurement of intracellular free Ca2+ concentration upon activation. Cells were pre-treated with 20 µΜ A740003 or control lock’s solution for 5 min respectively before administration with Bz-ATP. Triangle symbol indicates the addition of 50 µΜ Bz-ATP. The Fura-2 fluorescence at emission wavelength of 510 nm excited separately by wavelength of 340 or 380 nm were recorded, and the excitation ratio (340/380) was quantified at the indicated time point.
Biochem Biophys Res Commun

ACE2 contributes to the maintenance of mouse epithelial barrier function. [Abstract]

2020 Dec 17;533(4):1276-1282. PMID: 33097186
bioRxiv

2026 Jan 13.
Elife

Tachykinin signaling inhibits task-specific behavioral responsiveness in honeybee workers. [Abstract]

2021 Mar 24:10:e64830. PMID: 33760729

Fura-2 AM purchased from MedChemExpress. Usage Cited in: Elife. 2021 Mar 24:10:e64830. [Abstract]

Intracellular Ca2 levels of HEK293 cells expressing TRPR rose sharply in response to TRP2 and TRP3, based on fluorescence measures of the Ca2+ indicator Fura-2 AM. Hepes-buffered medium (HBM) was used as a control.
bioRxiv

2021 Jan 13.

용액&용해도

In Vitro:

DMSO : 10 mg/mL (9.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

0.9982 mL

4.9908 mL

9.9815 mL

5 mM

0.1996 mL

0.9982 mL

1.9963 mL

10 mM

---

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Odmara L Barreto-Chang, et al. Calcium imaging of cortical neurons using Fura-2 AM. J Vis Exp. 2009 Jan 19;(23):1067. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	0.9982 mL	4.9908 mL	9.9815 mL	24.9538 mL
DMSO	5 mM	0.1996 mL	0.9982 mL	1.9963 mL	4.9908 mL

Bioz

Keywords

Fura-2 AM

9 Cited Publications

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Minimum order quantity

100 mg

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Amount: USD 0.00

Biological Activity
- 제품 설명
- In Vitro
- Emission (Em)
- Excitation (Ex)
Chemical Information
Publications (9)
용액&용해도
- In Vitro
순도&문서
References

1/16
1/16

Fura-2 AM purchased from MedChemExpress. Usage Cited in: Purinergic Signal. 2024 Jun;20(3):273-284. [Abstract]

Customer Validation
The Ca2+ influx function of P2X7 in OCI-ly3 and SU-DHL-4 was validated by measurement of intracellular free Ca2+ concentration upon activation. Cells were pre-treated with 20 µΜ A740003 or control lock’s solution for 5 min respectively before administration with Bz-ATP. Triangle symbol indicates the addition of 50 µΜ Bz-ATP. The Fura-2 fluorescence at emission wavelength of 510 nm excited separately by wavelength of 340 or 380 nm were recorded, and the excitation ratio (340/380) was quantified at the indicated time point.
1/16

Fura-2 AM purchased from MedChemExpress. Usage Cited in: Elife. 2021 Mar 24:10:e64830. [Abstract]

Customer Validation
Intracellular Ca2 levels of HEK293 cells expressing TRPR rose sharply in response to TRP2 and TRP3, based on fluorescence measures of the Ca2+ indicator Fura-2 AM. Hepes-buffered medium (HBM) was used as a control.

상품명:
Cat. No.:
수량:
MCE Japan Authorized Agent:

상품명:
CAS No.:
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Purity:
수량:

Fura-2 AM

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Rhod-2 AM

Poloxamer 407 (F127)

Fura-2 AM (solution)

Poly-D-lysine hydrobromide (MW 70000-150000)

Thiazolyl Blue

Propidium Iodide

DAPI dihydrochloride

Publications Citing Use of MedChemExpress (MCE) Fura-2 AM

Biological Activity

Chemical Information

Publications (9)

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

Bioz

Fura-2 AM

Fluo-4 AM

Yoda 1

Thapsigargin

Capsaicin

Ionomycin

Rhod-2 AM

Poloxamer 407 (F127)

Fura-2 AM (solution)

Poly-D-lysine hydrobromide (MW 70000-150000)

Thiazolyl Blue

Propidium Iodide

DAPI dihydrochloride

Publications Citing Use of MedChemExpress (MCE) Fura-2 AM

Biological Activity

Chemical Information

Publications (9)

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

Fura-2 AM Related Classifications

HY-101897 Related Classifications

Bioz