LDS-751
Based on 1 Customer Validation
Lds-751 is a nucleic acid stain that mainly detects DNA. Lds-751 is a nucleic acid stain that mainly detects DNA. Lds-751 has a high affinity for DNA and fluorescence is enhanced after binding, but the maximum emission wavelength is 670nm. Lds-751 and Thiazole orange can be used for the differentiation of red blood cells, platelets, reticulocytes, and nucleated cells and can be stimulated at 488nm. Studies have shown that LDS-751 binds almost exclusively to mitochondria when incubated with nucleated living cells. After nucleated Acridine Orange (HY-101879) staining and LDS-751 treatment of cells, confocal microscopy revealed almost no co-location of the cells. Staining with Rhodamine 123 (HY-D0816), a dye known to bind polarized mitochondria, was almost identical to the pattern observed with LDS-751.
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- Purity: 99.07%
- CAS No.: 181885-68-7
- 화학식: C25H30ClN3O4
- 분자량:471.98
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보관:
-20°C, sealed storage, away from moisture and light
* The compound is unstable in solutions, freshly prepared is recommended.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
Biological Activity
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1.Preparation of LDS-751 working solution
1.1 Preparation of the stock solution:
Prepare LDS-751 stock solution with DMSO.
1.2 Preparation of LDS-751 working solution:
Dilute the stock solution in serum-free cell culture medium or PBS to obtain working solution.
Note: Please adjust the concentration of Lds-751 working solution according to the actual situation.
2. Cell staining
2.1 Cell preparation
For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Fix cells with 3.7% formaldehyde for 10 minutes, discard the fixative and rinsed with PBS for three times, 5 minutes each time.
2.3 Permeate cells with 0.2% Triton X-100 for 5 minutes, wash three times with PBS, 5 minutes each time.
2.4 Add 1 mL of LDS-751 working solution, and then incubate at room temperature for 1-5 minutes.
2.5 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
2.6 Wash twice with PBS, 5 minutes each time.
2.7 Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.
3. Precautions
3.1 It is recommended to store the stock solution at -20℃ or -80℃ away from light and avoid repetitive freeze-thaw cycles.
3.2 Detect the fluorescence as soon as possible to avoid fluorescence quenching.
3.3 It is recommended to use AntiFade Mounting Medium (MCE Cat. No.: HY-K1042) to slow the fluorescence quenching.
3.4 Please adjust the concentration of LDS-751 working solution according to the actual situation.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 181885-68-7
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Appearance Solid
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분자량 471.98
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화학식 C25H30ClN3O4
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Color Green to black
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SMILES
CC[N+]1=C2C=CC(N(C)C)=CC2=CC=C1/C=C/C=C/C3=CC=C(N(C)C)C=C3.O=Cl(=O)([O-])=O
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선적
Room temperature in continental US; may vary elsewhere.
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보관
-20°C, sealed storage, away from moisture and light
* The compound is unstable in solutions, freshly prepared is recommended.
용액&용해도
DMSO : 83.33 mg/mL (176.55 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
Blood samples (1 mL) are obtained using a sterile Butterfly-21 needle and plastic syringe from the antecubital vein of normal healthy volunteers who have given their informed consent. They are immediately transferred to plastic tubes containing 17.3 mg of phenylmethylsulphonyl fluoride (PMSF) and 1 mL of LDS-751 at room temperature. Aliquots (25 μL) are incubated for 5 min with between 2 μL and 5 μL of undiluted monoclonal antibodies, then diluted with 0.5 mL of 1% BSA in Hepesbuffered Hanks' balanced salts solution (HHBSS) and examined by flow cytometry[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
순도&문서
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Terstappen LW, et al. A rapid sample preparation technique for flow cytometric analysis of immunofluorescence allowing absolute enumeration of cell subpopulations. J Immunol Methods. 1989 Sep 29;123(1):103-12. [Content Brief]
[2]. Terstappen LW, et al. A rapid sample preparation technique for flow cytometric analysis of immunofluorescence allowing absolute enumeration of cell subpopulations. J Immunol Methods. 1989 Sep 29;123(1):103-12. [Content Brief]
[3]. Terstappen LW, et al. Five-dimensional flow cytometry as a new approach for blood and bone marrow differentials. Cytometry. 1988;9(6):548-556. [Content Brief]
[4]. McCarthy DA, et al. A simple flow cytometric procedure for the determination of surface antigens on unfixed leucocytes in whole blood. J Immunol Methods. 1993;163(2):155-160. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1187 mL | 10.5937 mL | 21.1873 mL | 52.9683 mL |
| 5 mM | 0.4237 mL | 2.1187 mL | 4.2375 mL | 10.5937 mL | |
| 10 mM | 0.2119 mL | 1.0594 mL | 2.1187 mL | 5.2968 mL | |
| 15 mM | 0.1412 mL | 0.7062 mL | 1.4125 mL | 3.5312 mL | |
| 20 mM | 0.1059 mL | 0.5297 mL | 1.0594 mL | 2.6484 mL | |
| 25 mM | 0.0847 mL | 0.4237 mL | 0.8475 mL | 2.1187 mL | |
| 30 mM | 0.0706 mL | 0.3531 mL | 0.7062 mL | 1.7656 mL | |
| 40 mM | 0.0530 mL | 0.2648 mL | 0.5297 mL | 1.3242 mL | |
| 50 mM | 0.0424 mL | 0.2119 mL | 0.4237 mL | 1.0594 mL | |
| 60 mM | 0.0353 mL | 0.1766 mL | 0.3531 mL | 0.8828 mL | |
| 80 mM | 0.0265 mL | 0.1324 mL | 0.2648 mL | 0.6621 mL | |
| 100 mM | 0.0212 mL | 0.1059 mL | 0.2119 mL | 0.5297 mL |
LDS-751 Related Classifications
HY-D0996 Related Classifications
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