Cabotamig
Based on 1 Customer Validation
Cabotamig is a humanized bispecific T-cell engager antibody targeting CDH17/CD3. Cabotamig is generated from anti-CDH17 monoclonal ARB102 by linking a CD3-binding scFv in the format of IgG4-scFv. Cabotamig can be used for the research of cancer, such as gastric cancer and colon cancers.
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- Purity: 99.88%
- CAS No.: 2755301-09-6
- 분자량:197 kDa
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보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
IgG1-G4-kappa-[scFv-heavy-kappa]2
Human
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CDH17 |
Cabotamig binds to CDH17 protein with high affinity (Kd = 10-10 M) and to CD3 protein with moderate affinity (Kd = 10-9 M), and binds to CDH17-expressing cells with a cellular affinity of 10-9 M and to CD3-expressing cells with a cellular affinity of 10-8 M[1].
Cabotamig selectively induces CDH17-dependent, T cell-mediated cytotoxicity of CDH17-positive gastrointestinal tumor cells, while CDH17-negative cancer cells and normal cells are not affected[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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IgG1-G4-kappa-[scFv-heavy-kappa]2
ELISA, FACS, Functional assay
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Flow Cytometry analysis of A549 cells labelling CDH17 (red) with Cabotamig (anti-CDH17) (HY-P990117). Cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG4 kappa (HY-P99003, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black). -
Flow cytometric analysis of 1X106 Juakat cells with Cabotamig (HY-P990730, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti-Human IgG H&L (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG4 (S228P) kappa (HY-P99003, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Chemical Information
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CAS No. 2755301-09-6
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Appearance Liquid
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분자량 197 kDa
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Color Colorless to light yellow
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Synonyms
ARB202
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선적
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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보관
Please store the product under the recommended conditions in the Certificate of Analysis.
순도&문서
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Data Sheet (257 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)